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Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay.

Quevedo Diaz, M A; Melnicáková, J; Kazár, J; Hoch, J; Senfleben, S; Lukácová, M.

Diagn Microbiol Infect Dis ; 42(4): 237-42, 2002 Apr.

Article in English | MEDLINE | ID: mdl-12007440

ABSTRACT

Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbance (A(492) and A(405/650)) values 2-5 times higher with SA than with EBs, resulting in a better discrimination between positive and negative human sera.

Subject(s)

Antibodies, Bacterial/blood , Antigens, Bacterial/chemistry , Chlamydia Infections/diagnosis , Chlamydia trachomatis/immunology , Enzyme-Linked Immunosorbent Assay/methods , Lipopolysaccharides/chemistry , Sarcosine/analogs & derivatives , Adult , Blotting, Western , Chlamydia trachomatis/chemistry , Detergents/chemistry , Electrophoresis, Polyacrylamide Gel , Female , Humans , Male , Middle Aged , Sarcosine/chemistry

ABSTRACT

Subject(s)

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