ABSTRACT
In analyzing aged samples by the AmpliType PM PCR amplification and Typing kit, it was occasionally observed that color developed typing strips had dark allele dots on PM loci but no visible S dot. Since the S dot acts as a minimum dot intensity control to determine positive alleles on the PM loci, it is necessary to apply another control system. To achieve positive PM typing from a degraded DNA sample that is inferred to be derived from a single donor, a standard has been adopted wherein loci from which sufficient PCR products are observed on agarose gel can be typed. The objective determination of sufficient PCR was done by comparison between band peak height of each locus generated from a sample and that of the corresponding locus generated from two nanograms (recommended minimum quantity as template DNA) of the control DNA provided in the kit.
Subject(s)
DNA Fingerprinting/methods , Tandem Repeat Sequences/genetics , Artifacts , Forensic Medicine/methods , Humans , Polymerase Chain Reaction , Reproducibility of Results , Specimen Handling , Time FactorsABSTRACT
A multiplex typing method of the tetrameric short tandem repeat (STR) loci TH11, D9S304, and D3S1744 was developed. The allelic ladder included alleles 6-11 (80-100bp) and 9.3 (95bp) for TH01, alleles 6-15 (125-161 bp) for D9S304, and alleles 13-22 (174-210bp) for D3S1744. The observed heterozygosity of D9S304 was 0.851. The combined discrimination power of the three loci was 0.991.
Subject(s)
Polymerase Chain Reaction/methods , Tandem Repeat Sequences/genetics , Base Sequence , DNA Primers , HeterozygoteABSTRACT
This study was performed to evaluate the physical resources for elite male cyclists of senior high school. Nine track cyclists (mean age, 17.4 years) and seven road cyclists (mean age, 17.3 years) were examined for body composition and cardiorespiratory function (VO2 max and O2 debt max), from 1988 to 1992. These measurements were compared with those of elite junior cyclists, and the progress of physiological function due to one-year training was examined for 7 cyclists. The results were as follows: 1. The mean parameters for track cyclists were recorded: percentage body fat (%Fat): 10.3%; lean body mass (LBM): 56.5kg; VO2 max: 3.93l/min, 62.5ml/kg.min; O2 debt max: 8.81l, 139.1ml/kg. 2. The mean parameters for road cyclists were recorded: %Fat: 10.9%; LBM: 54.9kg; VO2 max: 3.78l/min, 61.4ml/kg.min; O2 debt max: 7.89l, 128.4ml/kg. 3. The average %Fat, LBM, LBM/Ht, VO2 max and O2 debt max were not significantly different between track and road cyclists. 4. The average %Fat and VO2 max (ml/kg.min) of track and road cyclists were similar to those of elite junior cyclists. 5. By training for one year, the average VO2 max (ml/kg.min) and O2 debt max (ml/kg) showed a significant increase of approximately 13.6% and 22.6% respectively. These results indicated that through training 9 track and 7 road cyclists had achieved a superior body composition, aerobic work capacity and anaerobic work capacity, equal to those of elite junior cyclists.
