ABSTRACT
CaCwt1p is a Candida albicans putative transcriptional factor homologue to Rds2p in Saccharomyces cerevisiae. The lack of this protein in S. cerevisiae leads to a pleiotropic resistance to drugs and defects in cell wall architecture that are also detectable in C. albicans. It is also known that CaCwt1p is mainly expressed in the stationary growth phase of this fungus. In order to elucidate the role of CWT1, transcriptome analysis of the mutant strain was performed in exponential and stationary growth phases. A total of 460 genes were found to be up- or downregulated in the mutant strain growing exponentially, and 666 genes presented a misregulation when cwt1 cells reached the stationary phase. Under both conditions, 6% of the genes were related to cell wall architecture. An important set of genes involved in protein translation and ribosome biogenesis presented altered expression levels in cwt1 in both exponential and stationary growing cells. In addition, genes encoding for glycolytic enzymes and glycerol formation were found to be differentially regulated throughout cell growth. Finally, the expression of other transcriptional factors was modified in cwt1. This fact could indicate that the pleiotropic phenotype presented by the cwt1 null mutant is not only due to its absence, but also to the modified expression of other transcriptional factors. RSAT software was used to predict theoretical DNA binding motifs for this transcriptional factor. Surprisingly, the DNA sequences AGGGCT and/or AGCCCT could act as a direct promoting binding site for Cwt1p. These sequences have been reported to be related to the STRE box.
Subject(s)
Candida albicans/metabolism , Cell Wall/metabolism , Fungal Proteins/metabolism , Mutation , Oligonucleotide Array Sequence Analysis/methods , Proteome , Transcription Factors/metabolism , Binding Sites , Candida albicans/genetics , Candida albicans/growth & development , Fungal Proteins/genetics , Gene Expression Profiling , Gene Expression Regulation, Fungal , Glycolysis , Promoter Regions, Genetic , Protein Biosynthesis , Transcription Factors/geneticsABSTRACT
CandidaDB is a database dedicated to the genome of the most prevalent systemic fungal pathogen of humans, Candida albicans. CandidaDB is based on an annotation of the Stanford Genome Technology Center C.albicans genome sequence data by the European Galar Fungail Consortium. CandidaDB Release 2.0 (June 2004) contains information pertaining to Assembly 19 of the genome of C.albicans strain SC5314. The current release contains 6244 annotated entries corresponding to 130 tRNA genes and 5917 protein-coding genes. For these, it provides tentative functional assignments along with numerous pre-run analyses that can assist the researcher in the evaluation of gene function for the purpose of specific or large-scale analysis. CandidaDB is based on GenoList, a generic relational data schema and a World Wide Web interface that has been adapted to the handling of eukaryotic genomes. The interface allows users to browse easily through genome data and retrieve information. CandidaDB also provides more elaborate tools, such as pattern searching, that are tightly connected to the overall browsing system. As the C.albicans genome is diploid and still incompletely assembled, CandidaDB provides tools to browse the genome by individual supercontigs and to examine information about allelic sequences obtained from complementary contigs. CandidaDB is accessible at http://genolist.pasteur.fr/CandidaDB.
Subject(s)
Candida albicans/genetics , Databases, Genetic , Genome, Fungal , Candida albicans/pathogenicity , Fungal Proteins/chemistry , Fungal Proteins/genetics , Fungal Proteins/physiology , Genomics , Internet , User-Computer InterfaceABSTRACT
The Saccharomyces cerevisiae RPS0 A and B genes encode proteins essential for maturation of the 40S ribosomal subunit precursors. We have isolated a homologue of the RPS0 gene from Candida tropicalis, which we named CtRPS0. The C. tropicalis RPS0 encodes a protein of 261 amino acid residues with a predicted molecular weight of 28.65 kDa and an isoelectric point of 4.79. CtRps0p displays significant amino acid sequence homology with Rps0p from C. albicans, S. cerevisiae, Neurospora crassa, Schizosaccharomyces pombe, Pneumocystis carinii and higher organisms, such as human, mouse and rat. CtRPS0 on a high copy number vector can complement the lethal phenotype linked to the disruption of both RPS0 genes in S. cerevisiae. Southern blot analysis suggests that CtRPS0 is present at a single locus within the C. tropicalis genome.
Subject(s)
Candida/genetics , Fungal Proteins/genetics , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Genes, Fungal , Genetic Complementation Test , Molecular Sequence Data , Protein Processing, Post-Translational , Ribosomal Proteins/biosynthesis , Saccharomyces cerevisiae/genetics , Sequence Homology, Amino AcidABSTRACT
Phenotypic analysis was performed on six mutants of Saccharomyces cerevisiae deleted in one of the following open reading frames (ORFs), located on chromosome II: YBR254c, YBR255w, YBR257w, YBR258c, YBR259w and YBR266c. Disruption of the ORFs was carried out in the diploid strain FY1679 using the kanMX4 marker flanked by short sequences homologous to the target locus. Tetrad analysis following sporulation of the heterozygous disruptants showed that YBR254c and YBR257w are essential genes. YBR257w was later characterized and renamed POP4, its gene product being involved in 5.8S rRNA and tRNA processing (Chu et al., 1997). The tetrad analysis performed for the heterozygous disruptant for YBR266c showed that two of the four viable spores gave colonies of smaller size, reflecting a slower growth rate. Growth analysis of the disruptant haploids confirmed this defect at 30 degrees C and also at 15 degrees C. However, complementation tests failed to confirm that the deletion of YBR266c is responsible for this growth defect. Growth analysis of the disruptant haploid ybr255w(delta) showed a slower growth rate on YPD and minimal medium at 15 degrees C. Finally, no phenotypic effect could be detected associated to the disruption of ORFs YBR258c and YBR259w.
Subject(s)
Genes, Fungal , Saccharomyces cerevisiae/growth & development , Saccharomyces cerevisiae/genetics , Chromosomes, Fungal , Gene Deletion , Genetic Markers , Open Reading Frames/genetics , Phenotype , Polymerase Chain Reaction/methodsABSTRACT
Linkages of Ywp1 to other components of the Yarrowia lipolytica mycelial cell wall were studied by extraction with beta-mercaptoethanol and zymolyase (a beta-glucanase complex) and by the use of rabbit polyclonal antibody preparation raised against Ywp1. Ywp1 complexed with an N-glycosylated cell wall protein(s) to form supramolecular complexes through disulphide bridges (extractable with beta-mercaptoethanol) or bonded to beta-1,3-glucan (extractable with zymolyase). The lack of a specific morphological phenotype when YWP1 was knocked out by gene disruption might indicate that other proteins present in the cell wall of Y. lipolytica compensated for its loss. In this mutant, the electrophoretic pattern of proteins, detected with polyclonal antibodies against the entire cell wall, was different from that obtained with the parental strain, but sensitivity to calcofluor white, zymolyase and chitinase did not change. Quantitative analysis of fluorescence emitted by cells in the presence of fluorescent wheat germ agglutinin (FITC-WGA) indicated that chitin was organized in the cell wall of the mutant cells in a form different from that in the parental strain.
Subject(s)
Fungal Proteins/metabolism , Saccharomycetales/metabolism , Animals , Blotting, Western , Cell Wall/metabolism , Microscopy, Confocal , Phenotype , RabbitsABSTRACT
Urea or hot sodium dodecyl sulphate extracted a significant amount of the same proteins from the matrix of the cell wall of the yeast form and mycelial cells of Candida albicans. Gel filtration analysis of the urea-extracted proteins revealed that they occurred in the form of large complexes which were unaffected by up to 8 M urea. Among them, proteins en route to becoming covalently associated within the wall scaffold were identified by their reaction with specific antibodies. When urea was removed by dialysis, some of these proteins specifically reassociated into large aggregates which bound strongly with ConA, whereas others remained soluble in smaller associated products. The ability of some of these proteins to bind to the insoluble wall polysaccharides was also assessed. No self-assembling proteins were able to bind to glucans and/or chitin. Specificity of the binding to polysaccharides made of beta-bound glucosyl or N-acetylglucosaminyl residues was determined by the competitive effect of several disaccharides. Whereas laminaribiose and diacetylchitobiose were strong inhibitors of protein binding to both glucan and chitin, lactose, maltose and sucrose were ineffective.
Subject(s)
Candida albicans/metabolism , Chitin/metabolism , Fungal Proteins/metabolism , Glucans/metabolism , Antibodies, Fungal/chemistry , Binding, Competitive , Blotting, Western , Candida albicans/chemistry , Candida albicans/ultrastructure , Cell Wall/chemistry , Cell Wall/ultrastructure , Chromatography, Gel , Concanavalin A/chemistry , Electrophoresis, Polyacrylamide Gel , Membrane Proteins/metabolism , Microscopy, Electron , Microscopy, Fluorescence , Protein Binding/physiology , Sodium Dodecyl Sulfate/chemistry , Solubility , Urea/chemistryABSTRACT
A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4-kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl-tRNA synthetase beta subunit (FRS1) of Saccharomyces cerevisiae. We named it CaFRS1. It is located in a single copy in chromosome R, SfiI fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans, suggesting its requirement thereof in initial steps of the cell wall synthesis.
Subject(s)
Candida albicans/genetics , Genes, Fungal , Phenylalanine-tRNA Ligase/genetics , Amino Acid Sequence , Base Sequence , Blotting, Southern , Cell Wall/metabolism , Cloning, Molecular , Molecular Sequence Data , Phenylalanine-tRNA Ligase/physiologyABSTRACT
Candida albicans is an opportunistic fungal pathogen of humans. The cell wall of the organism defines the interface between the pathogen and host tissues and is likely to play an essential and pivotal role in the host-pathogen interaction. The components of the cell wall critical to this interaction are undefined. Immunoscreening of a lambda expression library with sera raised against mycelial cell walls of C. albicans was used to identify genes encoding cell surface proteins. One of the positive clones represented a candidal gene that was differentially expressed in response to changes in the pH of the culture medium. Maximal expression occurred at neutral pH, with no expression detected below pH 6.0. On the basis of the expression pattern, the corresponding gene was designated PRA1, for pH-regulated antigen. The protein predicted from the nucleotide sequence was 299 amino acids long with motifs characteristic of secreted glycoproteins. The predicted surface localization and N glycosylation of the protein were directly demonstrated by cell fractionation and immunoblot analysis. Deletion of the gene imparted a temperature-dependent defect in hypha formation, indicating a role in morphogenesis. The PRA1 protein was homologous to surface antigens of Aspergillus spp. which react with serum from aspergillosis patients, suggesting that the PRA1 protein may have a role in the host-parasite interaction during candidal infection.
Subject(s)
Antigens, Fungal/genetics , Candida albicans/immunology , Fungal Proteins/genetics , Receptors, Cell Surface , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA, Complementary , Gene Expression Regulation, Fungal , Hydrogen-Ion Concentration , Molecular Sequence Data , Phenotype , Sequence Homology, Amino AcidABSTRACT
Candidiasis are among the fungal infections the most difficult to diagnose and treat. Research focused on specific fungal components which are absent in the host, such as the cell wall has lead to a better understanding of Candida albicans pathogenicity and clinical impact. The cell wall is responsible for antigenic expression and primary interaction with the host. It is composed mainly of beta-glucans, chitin and mannoproteins, which account for the rigidity of the wall and for the fungal morphology. Of these components, mannoproteins might carry a "morphogenetic code" which might modulate the molecular architecture of the cell wall. The features of specific cell wall proteins as part of building blocks to form this structure is revised, and the usefulness of monoclonal antibodies obtained against cell wall components to study those processes, together with their clinical applicability, is discussed.
Subject(s)
Candida albicans/ultrastructure , Candidiasis/diagnosis , Candidiasis/drug therapy , Cell Wall/ultrastructure , Antibodies, Monoclonal , Cell Wall/immunology , Cell Wall/physiology , HumansABSTRACT
The YWP1 gene encoding a specific mycelial cell wall protein of Yarrowia lipolytica has been cloned and expressed in Saccharomyces cerevisiae using different episomal plasmids. Because the plasmids pYAE35BB and pYAE35ES carrying the YWP1 gene (including the 5' noncoding promoter sequences) failed to express it, the YWP1 gene was cloned under the control of GAL/CYC or ACT S. cerevisiae promoters. A main band with an apparent molecular mass of 70 kDa was detected by immunoblotting in the cell wall fraction of transformants. Ywp1 processing and incorporation to the cell wall were similar in both Y. lipolytica and S. cerevisiae but not in its final localization in the cell wall. In Y. lipolytica Ywp1 is covalently bound to the cell wall (it is released only by Zymolyase digestion), whereas in S. cerevisiae it was not (it was released by boiling SDS solutions). These results suggest that the sequences involved in recognition, anchoring of a protein to the cell wall, or the catalytic activities implicated are different, at least for Ywp1, in Y. lipolytica and S. cerevisiae. Another possibility is that the target for attachment of Ywp1 is missing or cryptic in the cell wall of S. cerevisiae.
Subject(s)
Ascomycota/chemistry , Fungal Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Cell Wall/metabolism , Fungal Proteins/chemistry , Fungal Proteins/genetics , Gene Expression , Glucan Endo-1,3-beta-D-Glucosidase/metabolism , Glycosylation , Molecular Weight , RNA, Fungal/analysis , RNA, Messenger/analysis , Recombinant Fusion Proteins , Saccharomyces cerevisiae/genetics , Sodium Dodecyl SulfateABSTRACT
We have isolated a 4.0-kb fragment from a genomic library of Candida albicans which contained two open reading frames (ORFs). One of them is homologous to a prolyl-tRNA synthetase that catalyses the charging of a specific tRNA by proline (CaPRS). A deduced sequence of 575 amino acids representing a polypeptide of 66.2 kDa was determined. A FASTA search indicated that the CaPRSp had an overall similarity of 54.4% with the product of a Saccharomyces cerevisiae ORF (YER087) and 43.8% with the prolyl-tRNA synthetase of Escherichia coli (COLIPRO). Consensus Class II aminoacyl-tRNA synthetase sequences were identified by the PROSITE program. CaPRS was localized to chromosome R of the C. albicans genome and CaPRS DNA hybridized to a major RNA transcript of 1.7 kb under all conditions tested.
Subject(s)
Amino Acyl-tRNA Synthetases/genetics , Amino Acyl-tRNA Synthetases/isolation & purification , Candida albicans/enzymology , Candida albicans/genetics , Genes, Fungal , Amino Acid Sequence , Amino Acyl-tRNA Synthetases/chemistry , Base Sequence , Chromosomes, Fungal/chemistry , Cloning, Molecular , DNA, Fungal/metabolism , Gene Expression Regulation, Fungal , Molecular Sequence Data , Open Reading Frames , Sequence Alignment , Sequence Homology, Amino AcidABSTRACT
In the course of an analysis of the functions and assembly of the cell wall of Candida albicans, we have cloned and characterized a gene, which we designated CSP37 (cell surface protein), encoding a 37-kDa polypeptide which is a membrane-associated protein. The gene was isolated by immunological screening of a DNA library constructed from mycelial cells with a polyclonal serum raised against cell walls of this morphology. Analysis of the nucleotide sequence of a corresponding genomic DNA fragment revealed a single open reading frame which encodes a predicted protein of 321 amino acids with no significant homology to others in the databases. Disruption of the CSP37 gene by the method described by Fonzi and Irwin (Genetics 134:717-728, 1993) eliminated expression of the Csp37 protein. The mutant strains showed no apparent defect in cell viability, growth, or cell wall assembly but displayed attenuated virulence in systemic infections induced in mice and reduced the ability to adhere to polystyrene.
Subject(s)
Candida albicans/genetics , Fungal Proteins/genetics , Membrane Proteins/genetics , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Cloning, Molecular , DNA, Fungal , Escherichia coli/metabolism , Gene Deletion , Genes, Fungal , Heterozygote , Homozygote , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Phenotype , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics , Sequence Analysis, DNAABSTRACT
The effect of immobilization on the cell wall of Saccharomyces cerevisiae cells was investigated in comparison with freely suspended batch grown cells. The pattern of mannoproteins released from the cell wall after Zymolyase digestion showed the presence of new mannoprotein species when cell growth takes place in a state of immobilization. The same result was obtained by exposure of freely suspended cells to a stressful concentration of ethanol, showing that two different adverse culture conditions induce a similar molecular response at the cell wall level.
Subject(s)
Ethanol/pharmacology , Membrane Glycoproteins/analysis , Saccharomyces cerevisiae/cytology , Cell Wall/chemistry , Cells, Immobilized/physiology , Glucan Endo-1,3-beta-D-Glucosidase , Membrane Glycoproteins/chemistry , Molecular Weight , Saccharomyces cerevisiae/chemistry , Saccharomyces cerevisiae/drug effectsABSTRACT
Cell wall extracts from the double-mutant mnn1 mnn9 strain were used as the immunogen to obtain a monoclonal antibody (MAb), SAC A6, that recognizes a specific mannoprotein--which we have named Icwp--in the walls of cells of Saccharomyces cerevisiae. Icwp runs as a polydisperse band of over 180 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of Zymolyase extracts of cell walls, although an analysis of the secretory pattern of the mannoprotein shows that at the level of secretory vesicles, it behaves like a discrete band of 140 kDa. Immunofluorescence analysis with the MAb showed that Icwp lies at the inner layer of the cell wall, being accessible to the antibody only after the outer layer of mannoproteins is disturbed by treatment with tunicamycin. The screening of a lambda gt11 expression library enabled us to identify the open reading frame (ORF) coding for Icwp. ICWP (EMBL accession number YLR391w, frame +3) codes for 238 amino acids, of which over 40% are serine or threonine, and contains a putative N-glycosylation site and a putative glycosylphosphatidylinositol attachment signal. Both disruption and overexpression of the ORF caused increased sensitivities to calcofluor white and Congo red, while the disruption caused an increased sensitivity to Zymolyase digestion, suggesting for Icwp a structural role in association with glucan.
Subject(s)
Antibodies, Monoclonal/immunology , Antigens, Fungal/genetics , Cell Wall/chemistry , Fungal Proteins/genetics , Membrane Glycoproteins/genetics , Membrane Glycoproteins/immunology , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/chemistry , Base Sequence , DNA, Complementary/genetics , Genes, Fungal , Glycosylphosphatidylinositols , Membrane Glycoproteins/metabolism , Molecular Sequence Data , Mutagenesis, Insertional , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/immunologyABSTRACT
We compared two phenotypic methods, colony morphotyping on Sabouraudtripheniltetrazolium agar (STTZ) and serotyping, with two genotypic methods, karyotyping and Random Amplified Polymorphic DNA bands obtained by PCR amplification (RAPD-PCR), for strain delineation in 33 Candida clinical isolates and two C. albicans strains from culture collections. Analysis of isolates on STTZ showed 11 different morphotypes. In two patients there was a switch in the morphotype coincidential with a change in the susceptibility of the isolates to azole antifungals. C. albicans isolates were divided into two serotypes. Sixteen and 18 different patterns were identified among the Candida isolates by karyotyping and RAPD-PCR, respectively. No relationship was found between any of the typing methods used and the source of the isolates. The combination of karyotyping and morphotyping on STTZ yielded useful epidemiological information, since it allowed the differentiation among the Candida species studied and the discrimination of clusters within C. albicans as well as to check the capacity of a strain to generate variants with different susceptibility to some antifungals.
Subject(s)
Candida/classification , Candidiasis/microbiology , Genotype , Karyotyping , Microbial Sensitivity Tests , Mycological Typing Techniques , Phenotype , Random Amplified Polymorphic DNA Technique , SerotypingABSTRACT
Cell wall regeneration of individual Candida albicans yeast and mycelial protoplasts was studied with confocal and electron microscopy using polyclonal antibodies and lectins. Quantitative measurements of the fluorescence emitted by individual protoplasts during the process of regeneration indicate that chitin is the first polymer to be laid down, whereas beta (1,3)- and beta (1,6)glucan are incorporated at a later stage. Mannoproteins were found on the surface of fresh protoplasts and those newly synthesized were then deposited with time. During the first steps of wall regeneration, the proteins that interacted covalently with chitin or glucan were different, but the same species were found linked to each polymer in yeast and mycelial regenerating forms. The aggregates formed by regenerating protoplasts were shown to be due to the chitin and mannoprotein network initially laid.
Subject(s)
Candida albicans/physiology , Protoplasts/physiology , Blotting, Western , Candida albicans/chemistry , Candida albicans/cytology , Cell Division , Cell Wall/chemistry , Cell Wall/physiology , Cell Wall Skeleton , Chitin/analysis , Electrophoresis, Polyacrylamide Gel , Fluorescent Antibody Technique, Indirect , Fungal Proteins/immunology , Glucans , Membrane Glycoproteins , Microscopy, Electron , Time FactorsABSTRACT
A cDNA clone specifying a cell wall protein was isolated from a Yarrowia lipolytica cDNA library. The cDNA library was constructed in the expression vector lambda gt 11, with the RNA isolated from actively growing mycelial cells. The deduced amino acid sequence shows that the encoded protein contains an N-terminal hydrophobic signal peptide. We have designated this protein YWP1 for Yarrowia lipolytica cell Wall Protein. Northern hybridization identified YWP1 transcript only when Y. lipolytica was growing in the mycelial form. The encoded protein seems to be covalently bound to the glucan cell wall since it is not released from the cell walls by sodium dodecyl sulphate extraction, but it is solubilized following partial degradation of beta-glucan by Zymolyase digestion. The protein is localized in the outer surface on the tip of the growing mycelial cells and is found partially cryptic in sub-apical locations, suggesting that it participates directly in the mycelial wall architecture.
Subject(s)
Cell Wall/chemistry , Cell Wall/genetics , Fungal Proteins/genetics , Saccharomycetales/chemistry , Yeasts/chemistry , Yeasts/genetics , Amino Acid Sequence , Antibodies, Fungal , Base Sequence , Blotting, Northern , Blotting, Southern , Blotting, Western , Cloning, Molecular , DNA, Complementary/genetics , DNA, Complementary/isolation & purification , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Fluorescent Antibody Technique, Indirect , Fungal Proteins/immunology , Gene Expression Regulation, Fungal , Gene Library , Genetic Vectors , Hydrolases/pharmacology , Molecular Sequence Data , Open Reading Frames , RNA, Fungal/genetics , Restriction Mapping , Saccharomycetales/genetics , Sodium Dodecyl Sulfate/pharmacology , Transcription, Genetic , Transformation, GeneticABSTRACT
Candida albicans cell wall components were analyzed by ethylenediamine (EDA) treatment. Based on their different solubility properties, the cell wall components produced three fractions (A, B, and C). Fractions B (EDA-soluble, water-insoluble) and C (EDA-insoluble) contained glucan, chitin, and protein in different proportions. After zymolyase (mainly a beta-glucanase complex) or chitinase treatment of fractions B and C, more polysaccharides and proteins were solubilized by a second EDA treatment, suggesting that the solubility of the polymers in EDA depends on the degree of polymer interactions. Western blot analysis using two monoclonal antibodies (1B12 and 4C12) revealed electrophoretic patterns that were similar in mycelial and yeast morphologies, except that in material obtained from mycelial walls, an additional band was detected with MAb 1B12. Fluorescence microscopy of cell wall fractions treated with FITC-labeled Con-A, Calcofluor white, and FITC-labeled agglutinin showed that glucan and mannoproteins are uniformly distributed in fractions B and C, while chitin is restricted to distinct patches. Transmission electron microscopy demonstrated that fraction C maintained the original shape of the cells, with an irregular thickness generally wider than the walls. When fraction C was treated with chitinase, the morphology was still present and was maintained by an external glucan layer, with an internal expanded fibrillar material covering the entire cellular lumen. Degradation of the glucan skeleton of fraction C with zymolyase resulted in the loss of the morphology.
Subject(s)
Candida albicans/chemistry , Cell Wall/chemistry , Ethylenediamines/pharmacology , Blotting, Western , Cell Wall/ultrastructure , Chitin/analysis , Chitinases/metabolism , Fungal Proteins/analysis , Glucans/analysis , Hydrolases/metabolism , Microscopy, Electron , Microscopy, Fluorescence , Polysaccharides/metabolismABSTRACT
Two plasmids (derived from YCplac22 and YEplac112) carrying a Candida albicans gene (including the 5' non-coding promoter sequences) coding for a 30 kDa membrane-bound protein, were used to transform Saccharomyces cerevisiae cells. A 30 kDa protein was immunodetected by Western blot in the membrane fraction of transformants. Northern analysis showed the presence of three mRNA species (of about 1.1, 0.7 and 0.5 kb) hybridizing with the C. albicans gene as a probe. The same result was obtained using the 5' and 3' regions of the gene as probes, whereas only a 1.1 kb mRNA was found in C. albicans and none was detected in S. cerevisiae control transformants. Thus, heterologous expression of this gene in S. cerevisiae results in a distinct pattern of mRNA processing, either due to the location on plasmid vectors and/or to differences in the mRNA processing systems in the two microorganisms.