ABSTRACT
BACKGROUND AND OBJECTIVES: Several studies have suggested that abnormal levels of serum cholesterol may be a major risk factor for osteoarthritis. However, no studies have been conducted to prevent osteoarthritis under controlled conditions of serum cholesterol. This study aimed to examine the relationship of sociodemographic and anthropometric characteristics, and nutrient and food intakes with osteoarthritis prevalence in Korean elderly subjects with controlled dyslipidaemia. METHODS AND STUDY DESIGN: This study included 314 subjects aged ≥65 years who were diagnosed and treated for dyslipidaemia (data from the Seventh Korea National Health and Nutrition Examination Survey, 2016). Among them, 108 were also diagnosed with osteoarthritis. Sociodemographic, health, and nutritional data were analysed. RESULTS: Osteoarthritis prevalence was higher in females, highereducated subjects, unmarried subjects, non-smokers, and subjects with high body mass index (p<0.05). After adjusting for the multiple variables, the non-osteoarthritis group had significantly higher vitamin C intake (132±11.0 vs 93.1±11.1 mg/day), fish intake (172±30.0 vs 79.0±12.9 g/day), and seaweed intake (93.7±19.3 vs 38.3±13.4 mg/day) than the osteoarthritis group. Furthermore, the lowest vitamin C, fish, seaweed intake group (quartile 1) each had 3.20, 2.76, 9.93 times higher risk of osteoarthritis than the highest vitamin C, fish, seaweed intake group (quartile 4) (p<0.05). CONCLUSIONS: Among Korean elderly subjects with controlled dyslipidaemia, those with osteoarthritis had lower vitamin C, fish, seaweed intakes than those without osteoarthritis. Although our results do not prove that low vitamin C, fish, seaweed intakes cause osteoarthritis, such relationship is worth exploring for a preventive perspective.
Subject(s)
Anthropometry , Dyslipidemias/drug therapy , Eating , Nutritional Status , Osteoarthritis/epidemiology , Osteoarthritis/etiology , Aged , Cross-Sectional Studies , Female , Humans , Hypolipidemic Agents/therapeutic use , Male , Nutrition Surveys , Socioeconomic FactorsABSTRACT
By causing long-term stimulation, gold wire implantation at acupoints has been used empirically to prolong the effects of acupuncture. This study shows that subcutaneous gold wire implantation at acupoints has long-term effects on bone regeneration in the rat ulna bone defect model.
Subject(s)
Acupuncture Therapy/instrumentation , Fracture Healing/physiology , Ulna Fractures/therapy , Acupuncture Points , Acupuncture Therapy/methods , Animals , Bone Regeneration/physiology , Gold , Implants, Experimental , Male , Physical Stimulation , Radiography , Rats , Rats, Sprague-Dawley , Time Factors , Ulna Fractures/diagnostic imaging , Ulna Fractures/pathologyABSTRACT
AIM OF THE STUDY: The root of Panax notoginseng (PN) is commonly used to treat chronic liver disease with its therapeutic abilities to stop haemorrhage in the circulation, while the PN flower (PN-F) is largely unknown in the biological activities on inflammation and mechanisms of its actions. In this study, the pharmacologic effects of PN-F methanol extract on inflammation were investigated to address potential therapeutic or toxic effects in LPS-stimulated mouse macrophage cells, RAW264.7 cells. MATERIALS AND METHODS: Production of NO, PGE2 and pro-inflammatory cytokines (TNF-alpha and IL-1beta) in supernatant, the expression of iNOS, COX-2 and cytokines, the phosphorylation of MAPK molecules (ERK1/2, JNK and p38 MAPK), and the activation of NF-kappaB in PN-F extract were assayed in LPS-stimulated RAW264.7 cells. RESULTS: PN-F extract significantly inhibited the productions of NO, PGE2, TNF-alpha and IL-1beta on the LPS-stimulated RAW264.7 cells. In addition, PN-F extract suppressed the mRNA and protein expressions of iNOS, COX-2, TNF-alpha and IL-1beta in LPS-stimulated RAW264.7 cells. The molecular mechanism of PN-F extract-mediated attenuation in RAW264.7 cells has close a relationship to suppressing the phosphorylation of MAPK molecules such as ERK1/2, JNK and p38 MAPK, and the translocation of NF-kappaB p65 subunit into nuclear. CONCLUSION: These results indicate that PN-F extract inhibits LPS-induced inflammatory response via the blocking of NF-kappaB signaling pathway in macrophages, and demonstrated that PN-F extract possesses anti-inflammatory properties in vitro.
Subject(s)
Anti-Inflammatory Agents/pharmacology , NF-kappa B/metabolism , Nitric Oxide Synthase Type II/antagonists & inhibitors , Panax notoginseng , Plant Extracts/pharmacology , Animals , Cell Line , Cyclooxygenase 2/metabolism , Dinoprostone/antagonists & inhibitors , Extracellular Signal-Regulated MAP Kinases/metabolism , Flowers , I-kappa B Kinase/metabolism , Inflammation , Interleukin-1beta/metabolism , JNK Mitogen-Activated Protein Kinases/metabolism , Lipopolysaccharides , Macrophages , Mice , Nitric Oxide/metabolism , Nitric Oxide Synthase Type II/metabolism , Signal Transduction/drug effects , Tumor Necrosis Factor-alpha/metabolism , p38 Mitogen-Activated Protein Kinases/metabolismABSTRACT
Matrix metalloproteinase-9 (MMP-9) degrades type IV collagen constituting the major structural component of the basement membrane and extra cellular membrane. The enzymatic activity is found to be elevated in tumor tissues. With the aim of finding novel MMP-9 inhibitors from natural products, 87 extracts of oriental medicinal herbs, which are used as prescriptions for cancer treatment in traditional Korean medicine, were screened for their inhibitory activities towards MMP-9. It was found that most of the hexane and chloroform fractions as well as water extracts showed a weak inhibitory effect on MMP-9 activity at a concentration of 100mug/ml. However, a strong inhibition was found in the butanol fractions of Cinnamomum cassia PRESL, Magnolia obovata THUEB., Magnolia officinalis REHD. et WILS., Magnolia officinalis REHD. et WILS. var. biloba REHD. et WILS., and Euonymus alatus (THUNB.) SIEB. with inhibitory activity (>90%) at a concentration of 100 microg/ml.
