ABSTRACT
Reports of the benthic dinoflagellate Ostreopsis spp. have been increasing in the last decades, especially in temperate areas. In a context of global warming, evidences of the effects of increasing sea temperatures on its physiology and its distribution are still lacking and need to be investigated. In this study, the influence of temperature on growth, ecophysiology and toxicity was assessed for several strains of O. cf. siamensis from the Bay of Biscay (NE Atlantic) and O. cf. ovata from NW Mediterranean Sea. Cultures were acclimated to temperatures ranging from 14.5 °C to 32 °C in order to study the whole range of each strain-specific thermal niche. Acclimation was successful for temperatures ranging from 14.5 °C to 25 °C for O. cf. siamensis and from 19 °C to 32 °C for O. cf. ovata, with the highest growth rates measured at 22 °C (0.54-1.06 d-1) and 28 °C (0.52-0.75 d-1), respectively. The analysis of cellular content of pigments and lipids revealed some aspects of thermal acclimation processes in Ostreopsis cells. Specific capacities of O. cf. siamensis to cope with stress of cold temperatures were linked with the activation of a xanthophyll cycle based on diadinoxanthin. Lipids (neutral reserve lipids and polar ones) also revealed species-specific variations, with increases in cellular content noted under extreme temperature conditions. Variations in toxicity were assessed through the Artemia franciscana bioassay. For both species, a decrease in toxicity was observed when temperature dropped under the optimal temperature for growth. No PLTX-like compounds were detected in O. cf. siamensis strains. Thus, the main part of the lethal effect observed on A. franciscana was dependent on currently unknown compounds. From a multiclonal approach, this work allowed for defining specificities in the thermal niche and acclimation strategies of O. cf. siamensis and O. cf. ovata towards temperature. Potential impacts of climate change on the toxic risk associated with Ostreopsis blooms in both NW Mediterranean Sea and NE Atlantic coast is further discussed, taking into account variations in the geographic distribution, growth abilities and toxicity of each species.
Subject(s)
Dinoflagellida , Global Warming , Temperature , Dinoflagellida/physiology , Dinoflagellida/growth & development , Mediterranean Sea , Harmful Algal Bloom , Animals , Acclimatization , Oceans and SeasABSTRACT
Marine microalgae have potential to be low-cost raw materials. This depends on the exploitation of different biomass fractions for high-value products, including unique compounds. Chrysochromulina rotalis, an under-explored haptophyte with promising properties, was the focus of this study. For the first time, C. rotalis was successfully cultivated in an 80 L tubular photobioreactor, illuminated by an easy-to-use light-emitting-diode-based system. C. rotalis grew without certain trace elements and showed adaptability to different phosphorus sources, allowing a significant reduction in the N:P ratio without compromising biomass yield and productivity. The design features of the photobioreactor provided a protective environment that ensured consistent biomass production from this shear-sensitive microalgae. Carotenoid analysis showed fucoxanthin and its derivatives as major components, with essential fatty acids making up a significant proportion of the total. The study emphasizes the tubular photobioreactor's role in sustainable biomass production for biorefineries, with C. rotalis as a valuable bioactive feedstock.
Subject(s)
Haptophyta , Microalgae , Photobioreactors , Carotenoids , BiomassABSTRACT
Antecedentes y objetivo: Las alteraciones motoras de la parálisis cerebral generan alteraciones del control postural y equilibrio. Los videojuegos y la realidad virtual trabajan sobre esos aspectos lúdicamente. El objetivo fue evaluar su efectividad en el control postural y equilibrio en población infantil con parálisis cerebral en Atención Temprana. Material y métodos: Entre enero y febrero del 2021 se realizó la búsqueda de ensayos clínicos aleatorizados y sin grupo control en Epistemonikos, Pubmed, Cochrane Library, Web of Science, Scopus, Science Direct, PEDro y Dialnet. Se revisó la bibliografía de guías clínicas, revisiones sistemáticas y metaanálisis. Se complementó con las revistas Fisioterapia, Cuestiones de Fisioterapia y Pediatric Physical Therapy. Tras la elección de artículos utilizándose la Declaración PRISMA, se analizó su calidad con las escalas CONSORT, TREND y SIGN. Resultados: Se incluyeron 16 estudios que evaluaban el control postural, equilibrio y función motora gruesa en población infantil entre 3 y 13 años con parálisis cerebral. La realidad virtual utilizada fue: Wii, XBOX, PlayStation2, TYROMOTION y ordenador. Los estudios obtuvieron mejoras significativas en variables que median el control postural, equilibrio y función motora gruesa al finalizar y se mantuvieron 4-8 semanas. No indicaron efectos adversos reseñables. La calidad fue variable incluyéndose estudios 1+ y 1− de la escala SIGN. Conclusiones: Los estudios reportan cierto grado de efectividad en la mejora del control postural y equilibrio en población infantil con parálisis cerebral en diferentes rangos de edad. Son necesarios estudios con mayor muestra, calidad y población centrada en Atención Temprana para generalizar estos resultados. (AU)
Background and objective: Motor disorders in cerebral palsy cause alterations in postural control and balance. Video games and virtual reality work on these aspects in a playful way. The objective was to evaluate their effectiveness on postural control and balance in children with cerebral palsy in Early Intervention.Material and methods: Between January and February 2021 we searched in Epistemonikos, Pubmed, Cochrane Library, Web of Science, Scopus, Science Direct, PEDro and Dialnet for randomised clinical trials and trials without a control group. Bibliographies of clinical guidelines, systematic reviews and meta-analyses were reviewed. It was complemented with the journals Fisioterapia, Cuestiones de Fisioterapia and Pediatric Physical Therapy. We use PRISMA Statement. After selecting articles, their quality was analysed using CONSORT, TREND and SIGN scales.Results: Sixteen studies were included that assessed postural control, balance and gross motor function in children aged 313 years with cerebral palsy. The virtual reality used was Wii, XBOX, PlayStation2, TYROMOTION and computer. The studies showed significant improvements in variables that evaluate postural control, balance and gross motor function at the end and maintained for 48 weeks. Motivation and participation levels increased. No notable adverse effects were reported. Quality was variable, including 1+ and 1− on the SIGN scale.Conclusions: Studies report a certain degree of effectiveness in improving postural control and balance in children with cerebral palsy in different age ranges. Studies with a larger sample, quality and a study population focused on Early Intervention are needed to generalise these results. (AU)
Subject(s)
Humans , Child, Preschool , Child , Cerebral Palsy , Postural Balance , Virtual Reality , Early Medical Intervention , Video Games , Randomized Controlled Trials as TopicABSTRACT
No disponible
Subject(s)
Humans , Male , Young Adult , Adult , Larva Migrans/diagnosis , Larva Migrans/therapy , Ivermectin/therapeutic use , Antiparasitic Agents/therapeutic use , Travel-Related Illness , ThailandSubject(s)
Aortic Aneurysm, Abdominal/complications , Intestinal Obstruction/etiology , Aged, 80 and over , Aortic Aneurysm, Abdominal/diagnostic imaging , Decompression, Surgical , Digestive System Surgical Procedures/methods , Humans , Intestinal Obstruction/diagnostic imaging , Intestinal Obstruction/surgery , Male , SyndromeSubject(s)
Abdomen, Acute/etiology , Appendix , Cecal Diseases/complications , Diverticulitis/complications , Adult , Humans , MaleABSTRACT
We present the data corresponding to the isolation and morphological and molecular characterization of a strain of Amphidinium carterae, isolated in Mallorca Island waters and now deposited in the microalgae culture collection of the Plant Biology and Ecology Department of the University of the Basque Country under the reference Dn241Ehu. The morphological characterization was made using two different techniques of microscopy and the molecular characterization by using the 28S rDNA sequences of D1 and D2 domains. This strain has been used for a culture study in an indoor LED-lighted pilot-scale raceway to determine its production of carotenoids and fatty acids, "Long-term culture of the marine dinoflagellate microalga Amphidinium carterae in an indoor LED-lighted raceway photobioreactor: Production of carotenoids and fatty acids." (Molina-Miras et al., 2018) [1].
