Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison.

In the field of nanotechnology, analytical characterization plays a vital role in understanding the behavior and toxicity of nanomaterials (NMs). Characterization needs to be thorough and the technique chosen should be well-suited to the property to be determined, the material being analyzed and the medium in which it is present. Furthermore, the instrument operation and methodology need to be well-developed and clearly understood by the user to avoid data collection errors. Any discrepancies in the applied method or procedure can lead to differences and poor reproducibility of obtained data. This paper aims to clarify the method to measure the hydrodynamic diameter of gold nanoparticles by means of Nanoparticle Tracking Analysis (NTA). This study was carried out as an inter-laboratory comparison (ILC) amongst seven different laboratories to validate the standard operating procedure's performance and reproducibility. The results obtained from this ILC study reveal the importance and benefits of detailed standard operating procedures (SOPs), best practice updates, user knowledge, and measurement automation.

Stochastic Particle Approach Electrochemistry (SPAE): Estimating Size, Drift Velocity, and Electric Force of Insulating Particles.

Stochastic particle impact electrochemistry (SPIE) is considered one of the most important electro-analytical methods to understand the physicochemical properties of single entities. SPIE of individual insulating particles (IPs) has been particularly crucial for analyses of bioparticles. In this article, we introduce stochastic particle approach electrochemistry (SPAE) for electrochemical analyses of IPs, which is the advanced version of SPIE; SPAE is analogous to SPIE but focuses on deciphering a sudden current drop (SCD) by an IP-approach toward the edge of an ultramicroelectrode (UME). Polystyrene particles (PSPs) with and without different surface functionalities (-COOH and - NH3) as well as fixed human platelets (F-HPs) were used as model IPs. From theory based on finite element analysis, a sudden current drop (SCD) induced by an IP during electro-oxidation (or reduction) of a redox mediator on a UME can represent the rapid approach of an IP toward an edge of a UME, where a strong electric field is generated. It is also found that the amount of current drop, idrop, of an SCD depends strongly on both the size of an IP and the concentration of redox electrolyte. From simulations based on the SPAE model that fit the experimentally obtained SCDs of three types of PSPs or F-HP dispersed in solutions with two redox electrolytes, their size distribution histograms are estimated, from which their average radii determined by SPAE are compared to those from scanning electron microscopic images. In addition, the drift velocity and corresponding electric force of the PSPs and F-HPs during their approach toward an edge of a Pt UME are estimated, which cannot be addressed currently with SPIE. We further learned that the estimated drift velocity and the corresponding electric force could provide a relative order of the number of excess surface charges on the IPs.

Optical experiment to test negative probability in context of quantum-measurement selection.

Negative probability values have been widely employed as an indicator of the nonclassicality of quantum systems. Known as a quasiprobability distribution, they are regarded as a useful tool that provides significant insight into the underlying fundamentals of quantum theory when compared to the classical statistics. However, in this approach, an operational interpretation of these negative values with respect to the definition of probability-the relative frequency of occurred event-is missing. An alternative approach is therefore considered where the quasiprobability operationally reveals the negativity of measured quantities. We here present an experimental realization of the operational quasiprobability, which consists of sequential measurements in time. To this end, we implement two sets of polarization measurements of single photons. We find that the measured negativity can be interpreted in the context of selecting measurements, and it reflects the nonclassical nature of photons. Our results suggest a new operational way to unravel the nonclassicality of photons in the context of measurement selection.

Stochastic Electrochemical Cytometry of Human Platelets via a Particle Collision Approach.

The quantitative analysis of human platelets is important for the diagnosis of various hematologic and cardiovascular diseases. In this article, we present a stochastic particle impact electrochemical (SPIE) approach for human platelets with fixation (F-HPs). Carboxylate-functionalized polystyrene particles (PSPs) are studied as well as a standard platform of SPIE-F-HPs. For SPIE-PSPs (or F-HPs), [Fe(CN)6]4- was used as the redox mediator, and electro-oxidation of [Fe(CN)6]4- to [Fe(CN)6]3- was conducted on a Pt ultramicroelectrode (UME) by applying a constant potential, where the corresponding oxidation current is mass-transfer-controlled. When PSPs (or F-HPs) are introduced into aqueous solution with [Fe(CN)6]4-, sudden current drops (SCDs) were observed, which resulted from the partial blockage of a Pt UME by collision of an individual PSP (or F-HP). For SPIE-PSPs (or F-HPs), we found that it is essential to enhance the migration of PSPs (F-HPs) toward a Pt UME by maximizing the steady state current associated with electro-oxidation of [Fe(CN)6]4-. This was accomplished by increasing its concentration to the solubility limit. We successfully measured the concentration of F-HPs dispersed in aqueous solution containing [Fe(CN)6]4- with a minimum detectable concentration of 0.1 fM, and the size distribution of F-HPs was also estimated from the obtained idrop distribution based on the SPIE analysis, where idrop stands for the magnitude of the current drop of each SCD. Lastly, we revealed that HPs without the fixation process (WF-HPs) are difficult to quantitatively analyze by SPIE because of their transient activation process, which results in changes from their spherical shape. The observed difficulty was also confirmed by finite element analysis, which shows that idrop can be significantly increased, as an elongated WF-HP is adsorbed on the edge of an UME.

Critical coupling in dissipative surface-plasmon resonators with multiple ports.

We theoretically investigate resonant absorption in a multiple-port surface-plasmon polaritons (SPP) resonator near the condition of critical coupling at which internal loss is comparable to radiation coupling. We show that total absorption is obtainable in a multiple-port system by properly configuring multiple coherent lightwaves at the condition of critical coupling. We further derive analytic expressions for the partial absorbance at each port, the total absorbance, and their sum rule, which provide a non-perturbing method to probe coupling characteristics of highly localized optical modes. Rigorous simulation results modeling a surface-plasmon resonance grating in the multiple-order diffraction regime show excellent agreements with the analytic expressions.