[Serological method of detecting the antigens of the causative agent of Sonne dysentery in lysates of the fecal inoculates from patients]. / Serologicheskii metod obnaruzhenii antigenov vozbuditelia dizenterii Zonne v lizatakh posevov isprazhenii bol'nykh.

Serological method of detection of Sh. sonnei antigens in the lysates of the patients, fecal cultures is suggested and approved. In the majority of cases of the results of bacteriological and serological methods of study of the feces coincided. Data confirming the specificity of the antibody neutralization test (ANT) in Sonne dysentery are presented. In connection with detection of the screening action of the Vi-antigen of typhoid bacilli there were elaborated additional methods for verifying the specificity of the ANT results. It is recommended to keep agar plates after selection of suspicious colonies during the bacteriological test; the lysate of the microbial crop should be additionally subjected to the ANT, this considerably increasing the percentage of laboratory confirmations of dysentery caused by Sh. sonnei.

[Intraepithelial shigellae invasion of the intestinal mucosa in patients suffering from acute Sonne dysentery]. / O vnutriépitelial'noi invazii shigell v slizistoi obolochke kishechnika u bol'nykh ostroi dizenteriei Zonne

Data are presented on detection of intraepithelial localization of shigellae in the biopsy materials of the large and small intestine mucosa of the patients with various forms of acute Sonne dysentery, in comparison with the clinico-morphological picture of the disease. Biopsy specimens (60 of the large and 7 of the small intestine) were stained with thionine and examined bacterioscopically; an intracellular distribution of the microbes was revealed in 58 cases; in 53 of them a subsequent identification of the microbes by the fluorescent antibody method was carried out; in 47 cases (88.7%) invasion of Sh. sonnei into the cells of the epithelium of the mucosa of the large and the small intestine was confirmed. There proved to be a direct relationship between the severity of the course of the disease, the level of shigellae localization and the extent of contamination by them of the cells of the large intestinal mucosa. The role of the penetrating capacity of shigellae in the development of the pathological process in dysentery is discussed.