ABSTRACT
Broad spectrum primers were used to amplify a fragment comprising the CP gene and putative ORF6 by RT-PCR from ds-RNA templates originating from 46 Portuguese varieties, totalling 190 samples, including some wild Vitis ssp sylvestris vines, and 2 vines from Slovenia. SSCP analysis was used as a preliminary screen to avoid cloning and sequencing very similar variants. Four groups of variants were recognized. In pair wise comparisons between nucleotide sequences the minimal homology found was 81%. In case of the cultivated varieties, no relationship could be seen between the phylogenetic groups and geographic origin or grape variety. Several isolates were found harbouring mixed infections with genomic variants from different groups, but the mixing did not lead to an extensive recombination between them. The deduced amino-acid sequences revealed a conserved CP subjected to strong purifying selection pressure. Analysis of the selection pressure operating on the putative ORF6 suggests that this ORF does not exist. Previously produced polyclonal antiserum raised against the recombinant CP of RSPaV expressed in Escherichia coli was shown to be able to detect all four groups of variants of RSPaV included in this study, which might enable the diagnosis of the virus on a serological basis.
Subject(s)
Capsid Proteins/genetics , DNA, Viral/analysis , Plant Diseases/genetics , Plant Viruses/genetics , Vitis/genetics , Genetic Variation , Genome, Viral , Molecular Sequence Data , Open Reading Frames , Phylogeny , Sequence Analysis, DNAABSTRACT
Este trabalho analisa 24 casos de cistos broncogenicos tratados nos Servicos de Cirurgia Toracica da Santa Casa de Misericordia do Rio de Janeiro, do Hospital Universitario Antonio Pedro e do Hospital Santa Cruz de Niteroi, de agosto de 1951 a maio de 1981. A idade dos pacientes variou de tres meses a 59 anos e houve predominancia do sexo masculino (18/6).Metade dos pacientes era assintomatica e 20% tinham infeccao respiratoria de repeticao. Onze cistos se localizavam no mediastino, enquanto que treze eram parenquimatosos. Todos os pacientes com sintomatologia de infeccao respiratoria de repeticao eram portadores de cistos parenquimatosos.Todos os pacientes foram operados por toracotomia postero-lateral.Em seis casos foi feita resseccao pulmonar enquanto que em todos os outros foi feita apenas resseccao do cisto
