[The structure of opportunistic microbiota of nasopharynx and vaginal tract in women with reproduction disorders and chronic endometritis.]

The study was carried out to investigate microbiocenosis of nasopharynx and vaginal tract in women with reproductive disorders and chronic endometritis. The females were separated on two groups. The first group included females with morphologically verified chronic endometritis (n=32). The second group included females without chronic endometritis (n=32). The chronic tonsillitis was established in 21 out of 65 females from first group and in comparison group only in 9 females. In the analyzed biotypes resemblance of main infection agents (staphylococcus,enterococсus, colibacillus) was established. In nasopharynx biotope normocenosis was established in first and second groups in 33.3% and 45% of cases correspondingly. The first degree of dysbiosis was determined in both groups with equal rate (55,5%), second degree of dysbiosis was established only in women with chronic endometritis and chronic tonsillitis (11.1%).

[The micro-ecological characteristic of intestinal biocenosis of children with functional disorders of gastrointestinal tract].

The study was carried out to investigate micro-ecological characteristics of intestinal microbiocenosis in children with functional disorders of gastro-intestinal tract. All patients were applied general clinical and laboratory examination (feces microscopy and analysis on intestinal dysbacteriosis). The micro-ecological coefficients were calculated i.e. permanence indicator and Jaccard's coefficient of community. The analysis of obtained data demonstrated that in children the risk factors of functional disorders of functioning of digestive organs are compromised heredity on diseases of digestive organs; frequent cold diseases; dysbiosis disorders in intestinal microbiota. In children with functional disorders of gastro-intestinal tract permanent microflora contained representatives of transitory opportunistic microflora entering into symbiotic relationships both with indigent microflora and with each other. The obtained data testify necessity of making in patients with functional disorders of digestive organs along with standard diagnostic procedures of evaluation of intestinal microflora. the reason is that main indices of micro-ecological balance of intestinal microbiota are important criteria reflecting severity of course of disease and effectiveness of applied treatment.

[THE SPECIES CHARACTERISTIC OF BIFIDOBACTERIA IN INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL DYSPEPSIA].

The study was carried out to investigate species specter and combinational variety of bifidobacteria in children with functional dyspepsia. The examined sampling included 73 children with functional dyspepsia aged from 4 to 6 years. The comparison group according common characteristic of microbiota of large intestine included 100 healthy children comparative by age. In 32 out of all examined children species specter of bifidobacteria in intestine was analyzed. The species diagnostic of bifidobacteria was implemented by using technique of polymerase chain reaction. In children with functional dyspepsia the analysis established dysbiosis alterations in intestinal biotope, characterizing by deficiency of bifidobacteria and selection of opportunistic microorganisms. The alteration of species structure of bifidobacteria in children with functional dyspepsia is demonstrated: decreasing of prevalence of populations of Bifidobacterium catenulatum and appearance of rare species of bifidobacteria B. dentium and B. angulatum. The differences are established concerning formation of species preference of bifidobacteria in conditions of conjoint vegetation in intestinal biotope of children with functional dyspepsia and in healthy children. The comprehensive analysis of micro-ecological status of intestine of children with functional dyspepsia using non-invasive techniques and including analysis of species profile, associative potential, communicative capacities of bifidobacteria complements complex of diagnostic criteria, permits to develop prognostic markers and to determine parameters of following therapy of functional dyspepsia.

[The formation of intestinal microbiocenosis in children in the industrial cities of Siberia].

The purpose of this study was to investigate the features of the formation of intestinal microbiocenosis in children residing in the industrial cities of Siberia. The study included 168 healthy children. There is presented the medical and hygienic assessment of the examined area with bearing in mind the degree and specificity of the man-made pollution of the atmosphere. There were revealed distinctive features of formation of intestinal microecology, specific for the each of the examined groups (diversity of species of transient microorganisms, rated characteristics of opportunistic pathogens, "indicator" species).

Synthesis of substituted 5[H]phenanthridin-6-ones as potent poly(ADP-ribose)polymerase-1 (PARP1) inhibitors.

1-, 2-, 3-, 4-, 8-, or 10-Substituted 5(H)phenanthridin-6-ones were synthesized and found to be potent PARP1 inhibitors. Among the 28 compounds prepared, some showed not only low IC(50) values (compound 1b, 10 nM) but also desirable water solubility characteristics. These properties, which are superior to the common PARP1 inhibitors such as benzamides and isoquinolin-1-ones, are essential for potential therapeutic usage. The variety of compounds allows SAR analysis of favored substituents and substituted positions on 5(H)phenanthridin-6-one ring.

Syntheses of 2-alkylamino- and 2-dialkylamino-4,6-diarylpyridines and 2,4,6-trisubstituted pyrimidines using solid-phase-bound chalcones.

Several substituted 2- and 4-hydroxyacetophenones are linked to Wang resin via a modified Mitsunobu protocol. These resin-bound acetophenones are condensed with aromatic aldehydes, and the resulting chalcones 5 are used for the synthesis of 2-dialkylamino- (9a-d) and 2-alkylamino-4,6-diarylpyridines (11a-f), and 2-alkyl-4,6-diaryl- (14a) and 2,4,6-triarylpyrimidines (14b,c) in a manner suitable for combinatorial applications.