ABSTRACT
We assessed the effects of some gefarnate analogs on gastric ulcer prophylaxis (adrenaline ulcers) and ulcer healing (acetic ulcers) and some secretory parameters in rats. Acute ulcers were induced in male Wistar rats by intraperitoneal injection of 2 mg/kg adrenaline hydrochloride. Rats were killed after 24 hours. Chronic gastric ulcers were induced in rats by application of 100% acetic acid to the serosal surface of the stomach on 60 sec. Gefarnate analogs introduced intraperitoneally or intragastrically. Gefarnate analogs dose-dependently increase the healing of gastric ulcers and have a marked prophylaxis effects especially in the case of adrenaline ulcers.
Subject(s)
Anti-Ulcer Agents/therapeutic use , Gefarnate/analogs & derivatives , Gefarnate/therapeutic use , Oxygen/chemistry , Stomach Ulcer/prevention & control , Animals , Anti-Ulcer Agents/chemistry , Disease Models, Animal , Gastric Mucosa/drug effects , Gefarnate/chemistry , Male , Molecular Structure , Rats , Treatment Outcome , Wound Healing/drug effectsSubject(s)
Energy Metabolism , Gerbillinae/metabolism , Kidney Medulla/metabolism , Water-Electrolyte Balance , Animals , Diuresis , Glycolysis , Kidney Concentrating Ability , Kidney Cortex/metabolism , Kidney Medulla/enzymology , L-Lactate Dehydrogenase/metabolism , Rats , Sodium-Potassium-Exchanging ATPase/metabolism , Species Specificity , Succinate Dehydrogenase/metabolismABSTRACT
Peptide analysis of tryptic hydrolysates of two lysozyme forms derived from oxidation of lysozyme with singlet oxygen shows that Trp-62, located at the active site, is destroyed. This is confirmed by the protective effect of the substrate (chitin), whose presense practically prevents the oxidation. A possibility of oxidating different tryptophan residues is discussed from the view-point of their availability to the reagent.
Subject(s)
Muramidase/metabolism , Oxygen/metabolism , Animals , Chitin/pharmacology , Chromatography, Affinity , Chromatography, Ion Exchange , Chromatography, Paper , Hydrolysis , Muramidase/analysis , Oxidation-Reduction , Peptides/analysis , Tryptophan/analysis , Tryptophan/metabolismABSTRACT
One out of six trytophan residues in two lysozyme modification, obtained under lysozyme photooxidation in the presence of methylene blue, is found to be oxidized to N'-formylkinurenine (in one modification) and to kinurenine (in the other modification). The transition of one modification into another via detaching of N'-formyl group by soft acid hydrolysis has shown that one and the same tryptophan residue is oxidized in both products, Possible mechanism of tryptophan oxidation to the products mentioned is discu-sed on the basis of the hypothesis on signlet mechanism of lysozyme photooxidation in the presence of methylene blue.
