ABSTRACT
Unusual serologic findings mimicking the B(A) phenomenon were noted in two group B blood donors using blended murine monoclonal anti-A reagent. With additional studies, including a binding experiment using affinity chromatography, the presence of aberrant group A activities was confirmed. This observation suggests that persons with atypical B(A) phenotype may warrant further investigation to delineate the ABO blood group.
Subject(s)
ABO Blood-Group System/genetics , Antibodies, Monoclonal , Blood Donors , Blood Grouping and Crossmatching/methods , Genetic Variation , N-Acetylgalactosaminyltransferases , ABO Blood-Group System/immunology , Adult , Chromatography, Affinity , Galactosyltransferases/blood , Hemagglutination Tests , Humans , Indicators and Reagents , MaleABSTRACT
A case of paroxysmal cold hemoglobinuria (PCH) occurring in a patient with Klebsiella pneumonia is described. The first sign of PCH in the patient was erythrophagocytosis. The Donath-Landsteiner (D-L) antibody exhibited anti-P specificity both serologically and biochemically. The appearance of D-L antibody during fulminating Klebsiella pneumonia strongly suggests their causal relationship.
