ABSTRACT
To estimate the efficiency of proline dehydrogenase gene suppression towards increasing of sunflower (Helianthus annuus L.) tolerance level to water deficit and salinity, we employed strain LBA4404 harboring pBi2E with double-stranded RNA-suppressor, which were prepared on basis arabidopsis ProDH1 gene. The techniques of Agrobacterium-mediated transformation in vitro and in planta during fertilization sunflower have been proposed. There was shown the genotype-depended integration of T-DNA in sunflower genome. PCR-analysis showed that ProDH1 presents in genome of inbred lines transformed in planta, as well as in T1- and T2-generations. In trans-genic regenerants the essential accumulation of free L-proline during early stages of in vitro cultivation under normal conditions was shown. There was established the essential accumulation of free proline in transgenic regenerants during cultivation under lethal stress pressure (0.4 M mannitol and 2.0% sea water salts) and its decline upon the recovery period. These data are declared about effectiveness of suppression of sunflower ProDH and gene participation in processes connected with osmotolerance.
Subject(s)
Agrobacterium/genetics , Genes, Suppressor , Genetic Vectors , Helianthus/genetics , Proline Oxidase/genetics , RNA, Double-Stranded/genetics , Transformation, Genetic , Gene Transfer Techniques , Genes, Plant , Helianthus/enzymology , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , Seeds/enzymology , Seeds/geneticsABSTRACT
The effect of vanadium oxyanions on genome of soybean (Glycine max L., Merr.) in tungsten-resistant cell line was studied. The line is resistant to V(5+)-oxyanions. RAPD-amplicons with identical length are differentially synthesized from DNAs of tungsten-resistant cell line as well as of the initial culture was shown by using the lethal dose of oxyanions W6+ and then V5+. The presence of instable loci in soybean genome under different stressors is discussed.
