ABSTRACT
AIM: Intestine microbiocenosis structure, endotoxinemia level, C-reactive protein (CRP) and TNFalpha concentration in chronic heart failure patients (CHF) were studied depending on disease severity. MATERIALS AND METHODS: Clinical, echocardiographic and laboratory examination of 104 patients was performed: 75 CHF patients and 29 healthy volunteers. Laboratory examination included bacteriologic analysis of feces for dysbiosis, determination of endotoxin (ET) levels, CRP and TNFalpha levels in blood serum. RESULTS: More expressed alterations in large intestine microbiocenosis and an increase of endotoxinemia and systemic inflammation factors (TNFalpha and CRP) levels were detected in patients with CHF in comparison to individuals without circulatory system pathology. CHF progression is associated with an increase of intestine dysbiosis, and ET, TNFalpha and CRP levels in blood. CONCLUSION: Endotoxinemia in CHF patients, that is caused by an increase in intestine wall permeability during development of venous congestion in systemic circulation, results in immune system activation manifesting in an increase of systemic inflammation factor level in blood that can aggravate the CHF course.
Subject(s)
Endotoxemia/microbiology , Heart Failure/microbiology , Inflammation/microbiology , Intestines/microbiology , Biomarkers/blood , C-Reactive Protein/analysis , Endotoxemia/blood , Endotoxemia/complications , Endotoxins/blood , Enterobacteriaceae/isolation & purification , Escherichia coli/isolation & purification , Escherichia coli/pathogenicity , Female , Heart Failure/complications , Humans , Inflammation/blood , Inflammation/complications , Male , Middle Aged , Tumor Necrosis Factor-alpha/bloodABSTRACT
The proposed method is intended for single-stage detection of A and B antigens in ultrafine hair fragments by means of absorption-elution and mixed agglutination techniques with the use of a mixture of anti-A and anti-B sera.
