The role epiphytes play in particle capture of seagrass canopies.

Seagrass epiphytic communities act as ecological indicators of the quality status of vegetated coastal environments. This study aims to determine the effect leaf epiphytes has on the sediment capture and distribution from outside sources. Thirteen laboratory experiments were conducted under a wave frequency of 0.5 Hz. Three epiphyte models were attached to a Zostera marina canopy of 100 plants/m2 density. The sediment deposited to the seabed, captured by the epiphytic leaf surface, and remaining in suspension within the canopy were quantified. This study demonstrated that the amount of epiphytes impacts on the sediment stocks. Zostera marina canopies with high epiphytic areas and long effective leaf heights may increase the sediment captured on the epiphyte surfaces. Also, reducing suspended sediment and increasing the deposition to the seabed, therefore enhancing the clarity of the water column. For largest epiphytic areas, a 34.5% increase of captured sediment mass is observed. The sediment trapped on the leaves can be 10 times greater for canopies with the highest epiphytic areas than those without epiphytes. Therefore, both the effective leaf length and the level of epiphytic colonization are found to determine the seagrass canopy ability at distributing sediment.

Suspended sediments mediate microplastic sedimentation in unidirectional flows.

Microplastic particles (MP) are an emerging contaminant threatening many aquatic systems. Because of the sharp increase in plastic manufacture, the concentration of MP in natural ecosystems has grown dramatically. While it is known that when MP enter aquatic ecosystems they are transported and dispersed via different mechanisms (currents, waves, turbulence), the processes involved are still poorly understood. In the current study, the transport of MP by a unidirectional flow has been investigated in a laboratory flume. MP enter the system through a plume that can (or not) have suspended sediment. The interaction between MP and sediment was studied for three different MP particle types (Polyamide (PA) and Polyvinyl Chloride (PVC) fragments, and Polyethylene Terephthalate (PET) fibers), and four different sediment concentrations (0 g/l, 15 g/l, 30 g/l and 45 g/l). In all cases, sediment increased the vertical transport of MP to the bottom. The greater the sediment concentration, the greater the downward flux of MP. Sediment particles scavenged PA fragments downwards at the highest rate, followed by PET fibers and finally PVC fragments. These results indicate that a sediment particle-laden plume carrying MP may induce a differential settling of MP as they are advected. The scavenging of MP by sediments may result in sedimentation segregated patterns, with MP being found at shorter distances than expected for the case without sediment, therefore increasing the presence of MP near their contaminant sources.

Spatial sedimentation and plant captured sediment within seagrass patches.

Habitat degradation in coastal ecosystems has resulted in the fragmentation of coastal aquatic vegetation and compromised their role in supplying essential ecological services such as trapping sediment or sequestering carbon. Fragmentation has changed seagrass architecture by decreasing the density of the canopy or engendering small patches of vegetated areas. This study aims to quantify the role different patch sizes of vegetation with different canopy densities have in the spatial distribution of sediment within a patch. To this aim, two canopy densities, four different patch lengths, and two wave frequencies were considered. The amounts of sediment deposited onto the bed, captured by plant leaves, remaining in suspension within the canopy, and remaining in suspension above the canopy were used to understand the impact hydrodynamics has on sediment distribution patterns within seagrass patches. In all the cases studied, patches reduced the suspended sediment concentrations, increased the capture of particles in the leaves, and increased the sedimentation rates to the bed. For the lowest wave frequency studied (0.5 Hz), the sediment deposited to the bottom was enhanced at canopy edges, resulting in spatial heterogeneous sedimentation patterns. Therefore, restoration and preservation of coastal aquatic vegetation landscapes can help face future climate change scenarios where an increase in sedimentation can help mitigate predicted sea level rise in coastal areas.

Stem stiffness functionality in a submerged canopy patch under oscillatory flow.

Seagrass canopies are coastal ecosystems that are able to modify the abiotic environment through their architectural structure. They have different structural parameters, such as plant stem stiffness, patch length and canopy density, all of which determine their overall functionality in modifying the seafloor hydrodynamics within coastal areas. To determine the interaction between hydrodynamics and the canopy structure, a set of laboratory experiments were carried out with both rigid and flexible stems for different canopy densities, patch lengths and wave frequencies. In the upper part of the canopy, flexible plants move with the flow without generating drag or producing turbulent kinetic energy, while rigid plants generate drag and produce turbulent kinetic energy. In the inner canopy layer, both types of plants behave like rigid stems and produce turbulent kinetic energy. A non-dimensional model based on the turbulent kinetic energy, the wave velocity and the plant characteristics is presented to describe the behaviour of flexible and rigid plants under an oscillating flow. Flexible plants behave in a stiffer manner under high wave frequencies than under low wave frequencies, thus making their behaviour closer to that of rigid plant stems. This difference between both canopy structures can explain their distribution in the environment, with rigid canopies being more extended in more sheltered regions while flexible plants are characteristic of more exposed regions with high flow energy.

Vertical distribution of microplastics in water bodies causes sublethal effects and changes in Daphnia magna swimming behaviour.

Plastic debris has been found to be ubiquitous in many aquatic ecosystems and is constantly accumulating, not only because more and more plastic is being rapidly released into the environment, but also because its slow degradation means it persists in the water. Some more buoyant plastics accumulate in the water column, whereas other heavier types sink to the bottom. Consequently, the presence of microplastics can threaten organisms living in the water column as well as those found in the benthic zone. In this study, the filter feeder Daphnia has been found to ingest microplastics as the particle diameter (< 30 µm) is within their edible particle size range and they are unable to differentiate between particles of different natures. Four different treatments were considered: food only; only microplastic particles; 50% food and 50% microplastic particles; neither food nor microplastics. Sinking microplastics have been found to decrease Daphnia magna individuals' swimming velocity during vertical or cruising swimming trajectories, therefore demonstrating the sublethal effects microplastics have on this organism. In addition, microplastics decreased their body growth and survival rates. In cases with the presence of only microplastics, the swimming trajectories of Daphnia indicated the most serious stress experienced as individuals reversed vertical or cruising swimming trajectories to hopping and sinking movements. Therefore, Daphnia individuals in freshwater systems polluted by microplastics might take on the role of ingesting them and later on transporting them to deeper layer water column. In this way microplastics that would remain in the water column for a long time due to their buoyancy, might accumulate at the bottom of the water column.

Functional dynamics of vegetated model patches: The minimum patch size effect for canopy restoration.

For the past two centuries coastal zones have been suffering seagrass loss resulting in a network of vegetated patches which are barely interconnected and which may compromise the ecological services provided by the canopy. To optimize management efforts for successful restoration strategies, questions need to be addressed about what appropriate canopy architectural considerations are required under certain hydrodynamic conditions. In this study, a set of laboratory experiments were conducted in which hydrodynamic conditions, plant densities and vegetated patch lengths were varied to determine minimum patch lengths for successful management strategies. Based on the TKE production, this study finds two possible canopy behaviours of seagrasses under oscillating flows: one where plants do not interact with the flow and the other where they interact with waves and produce TKE. A threshold from the first to second behaviour occurs for [Formula: see text] = 2, where CD is the drag of the vegetated patch, n is the number of stems per m2, d is the stem diameter and ϕ is the solid plant fraction. Therefore, high canopy densities, large patches of vegetation or moderate wave velocities will produce plant-wave interaction, whereas low canopy densities, small vegetation patches or slow wave velocities will produce a behaviour akin to the non-vegetated cases.

Zooplankton-based reactors for tertiary wastewater treatment: A pilot-scale case study.

Nature-based wastewater treatments are an economic and sustainable alternative to intensive technologies in rural areas, although their efficiency needs to be improved. This study explores technological co-operation between zooplankton (e.g., Daphnia magna) and bacterial and algal biofilms in a 1.5 m3 zooplankton-based reactor for the on-site treatment of secondary urban wastewater. The efficiency of the reactor was evaluated over a 14-month period without any maintenance. The results suggest a low seasonality effect on nutrient polishing (organic matter and nitrogen) and the removal of solids (TSS and turbidity). The best performance, involving a decrease in organic carbon, nitrogen, E. coli loads, and solid content was achieved in winter when operating the reactor at 750 L d-1. Under these conditions, the quality of the effluent water was suitable for its reuse for six different purposes in conformance with Spanish legislation. These results demonstrate that the zooplankton-based reactor presented here can be used as an eco-sustainable tertiary treatment to provide water suitable for reuse. On-site research revealed that the robustness of the reactor against temperature and oxygen fluctuations needs to be improved to ensure good performance throughout the year.

An Anthocyanin-Rich Extract Obtained from Portuguese Blueberries Maintains Its Efficacy in Reducing Microglia-Driven Neuroinflammation after Simulated Digestion.

Dietary polyphenols are multi-target compounds that have been considered promising candidates in strategies for the mitigation of neurological diseases, acting particularly through reduction of microglia-driven neuroinflammation. In this study, an anthocyanin-rich extract obtained from Portuguese blueberries was subjected to a simulated gastrointestinal digestion; after chemical characterisation, the potential of both non-digested and digested extracts to combat neuroinflammation was evaluated using a microglia N9 cell line. Although the extracts have markedly different chemical composition, both were efficient in reducing the production of either key inflammatory markers or reactive oxygen species and in enhancing reduced glutathione levels in activated cells. Furthermore, this protection was shown to be related to the suppression of nuclear factor kappa B (NF-kB) activation, and to a signal transducer and activator of transcription 1 (STAT1)-independent mechanism. These results demonstrate that the anthocyanin extract, after simulated digestion, maintains its efficacy against neuroinflammation, and can, therefore, assume a relevant role in prevention of neuroinflammation-related neurological disorders.

Synergistic effects of water temperature, microplastics and ammonium as second and third order stressors on Daphnia magna.

Daphnids, including the water flea Daphnia magna, can be exploited for wastewater treatment purposes, given that they are filter feeder organisms that are able to remove suspended particles from water. The presence of pollutants, such as microplastics and chemicals, might be considered stressors and modify the behaviour and survival of D. magna individuals. The impact of the cumulative pollutants that regulate the fate of living organisms has yet to be fully determined. Here we present the effect of double and triple combinations of stressors on the behaviour of D. magna. The impact of water temperature, ammonium and polystyrene microplastics on the filtration capacity and survival of D. magna is studied. Water temperatures of 15 °C, 20 °C and 25 °C, microplastic-to-food ratios of 25% and 75%, and ammonium concentrations of 10 and 30 mg N-NH4+ L-1 are tested after making dual and triple combinations of the parameters. A synergistic effect between water temperature and ammonium is normally observed but not in the case of the lower values of ammonium concentration and temperature. The combination of three stressors (water temperature, microplastics and ammonium) is also found to be synergistic, producing the greatest impact on D. magna filtration capacity and reducing their survival. In comparison with the effect of the two stressor conditions, the combination of the three stressors caused a reduction of between 13.1% and 91.7% in the t50% time (the time required for a 50% reduction in the D. magna filtration capacity) and a reduction of between 4.8% and 54.5% in TD50 (the time for 50% mortality).

Assessment of zooplankton-based eco-sustainable wastewater treatment at laboratory scale.

The combination of the filtration capacity of zooplankton (e.g. Daphnia) with the nutrient removal capacity of bacterial/algal biofilm in a zooplankton-containing reactor could provide a natural-based alternative for wastewater treatment. A laboratory-scale zooplankton-based reactor was tested at different HRTs resulting in a significant reduction in nutrient concentrations in wastewater when the system was operated at HRTs longer than 1.1 days (preferably of between 2 and 4 days). However, the presence of high concentrations of organic matter (>250 mg COD L-1) in the wastewater inhibited zooplankton activity, limiting its use to tertiary treatment. Therefore, in combination with other natural treatments that can perform primary and secondary treatments, zooplankton may provide a solution for wastewater clarification and nutrient polishing. The effect of a common metal such as copper on the filtration capacity of Daphnia was also evaluated. Daphnia, as well as the whole zooplankton-based reactor, adapted to copper concentrations of up to 70 µg Cu L-1 but an overload of 380 µg Cu L-1 for two-weeks severely affected the biological system.

Particle size segregation of turbidity current deposits in vegetated canopies.

Interactions between ecology, hydrodynamics and sediments play central roles in the evolution of coastal and freshwater ecosystems. We set out to characterise interactions of a specific hydrodynamic phenomenon - turbidity currents - with vegetation and sediment dynamics. We measured hydrodynamics and sediment deposition rates when turbidity currents flowed into plant canopies in a lock-exchange flume experiment, using simulated vegetation and three real plant species, and varying the turbidity current's initial sediment concentration. The natural sediment used had an essentially bimodal size distribution, with coarse (6.2-104 µm) and fine (2.2-6.2 µm) fractions. In all cases, on entering the vegetation canopy, the turbidity current was initially inertially-dominated, but subsequently became drag-dominated. In the inertial regime, there was no size segregation in the deposited material. In the drag-dominated regime, the deposited material became increasingly dominated by fine sediment, at a rate dependent on the vegetation type. The transition between these two regimes occurred at a distance equivalent to 5.1-7.6 times the total water depth downstream of the lock gate. The size segregation of deposited sediment is posited to have consequences for substrate evolution, which in turn may affect vegetation growth. Thus, our findings point to a non-linear feedback mechanism between the spatial heterogeneity of vegetation canopies and that of the substrate they help to engineer.

Optimal light conditions for Daphnia filtration.

Daphnia populations are present in lakes and ponds. They are known to experience diurnal vertical migrations according to their feeding needs. During the day they migrate downwards to avoid predation in light-receiving layers and at night they migrate upwards, searching for food in the shallow productive layers. The light photoperiod and light intensity vary depending on the latitude and, therefore, the precise location of lakes and ponds will be an additional and crucial parameter in determining the development of Daphnia. Here we will focus on a population of Daphnia magna (a genus of the Cladocera order). The effect of both light intensity and photoperiod on Daphnia filtration was studied in laboratory experiments. An increase in the light intensity resulted in two D. magna responses depending on the exposure time of individuals to light. Short time exposures to a decrease in the light intensity of less than one day produced an increase in the D. magna filtration. However, exposures of longer than one day resulted in a decrease in the D. magna filtration along with a decrease in the light intensity. Photoperiod exposures of 8, 12 and 16 h produced greater D. magna filtrations than photoperiods of 0, 4 and 24 h. In this study, regulation of the light intensity and the period of exposure were used in laboratory experiments to establish D. magna development thresholds by latitudinal variation in the photoperiod.

Mediated food and hydrodynamics on the ingestion of microplastics by Daphnia magna.

There is consensus on the need to study the potential impact microplastics (MP) have on freshwater planktonic organisms. It is not yet fully understood how MP enter the aquatic food web or the effect they have on all the trophic levels. As a result of the potential for MP to accumulate throughout food webs, there is increasing interest in evaluating their fate in a variety of environmental conditions. This study investigated the variability in the ingestion of MP to food ratios and the exposed time of MP to Daphnia magna in non-sheared and sheared conditions. The sheared environment provided Daphnia magna with the conditions for optimal filtering capacity. Regardless of the ratios of MP concentration to food concentration (MP:Food), the filtration capacity of the Daphnia magna was enhanced in the sheared experiments. In both the sheared and non-sheared experiments, filtration capacity decreased when the ratios of MP to food concentration and the exposure times to MP were increased. Mortality was mainly enhanced in the non-sheared conditions at higher MP concentrations and exposure times to MP. No mortality was found in the sheared conditions for the exposure times studied. Therefore, in aquatic systems that undergo constant low sheared conditions, Daphnia magna can survive longer when exposed to MP than in calm conditions, provided food concentrations do not limit their capacity to filter.

Functional responses of Daphnia magna to zero-mean flow turbulence.

Daphnia are important to understanding the biogeochemistry of aquatic ecosystems, mainly because of their ability to filter bacteria, algae and inorganic particles as well. Although there are many studies on the general effects that biotic and abiotic stressors, increased temperature and hypoxia, salinity, metals, pharmaceuticals, pesticides, etc., have on Daphnia populations, little is known about the impact elevated turbulence has. Here, we show that turbulence affects Daphnia magna survival, swimming behaviour and filtering capacity. Our data demonstrate that altering their habitat by induced mixing from turbulence, induces an increased filtering capacity of the Daphnia magna individuals, provided the level of background turbulence (defined by the dissipation of turbulent kinetic energy) is lower than ε = 0.04 cm2 s-3. The filtering capacity reduced exponentially with increasing ε, and at ε > 1 cm2 s-3 both mobility and filtration were suppressed and eventually led to the death of all the Daphnia magna individuals.

Daphnia magna filtration, swimming and mortality under ammonium, nitrite, nitrate and phosphate.

Biological methods are a promising approach to treating wastewater in order to produce water of an appropriate quality for sub-potable water purposes, thus reducing pressure on potable water sources. Daphnia magna are organisms that filter on small suspended particles and bacteria and so may be able to clarify and disinfect wastewater. However, Daphnia magna are sensitive to common chemicals and might be vulnerable to the quality of the wastewater. This study analyses the filtration, mobility and mortality rates of Daphnia magna exposed to seven days of changing concentrations of ammonium, nitrite, nitrate and phosphate. Inactivation increased with the time of exposure for both nitrite and ammonium, with a 50% inactivation in Daphnia magna filtrations after 7 days of exposure at nitrite concentrations above 6 ppm and ammonium concentrations above 40 ppm. The Daphnia filtration remained unaltered in the nitrate and phosphate concentrations. Mortality increased with nitrite and ammonium concentrations, but not with phosphate or nitrate. The swimming velocity of Daphnia magna individuals decreased when both nitrite and ammonium concentrations increased and also with phosphate concentrations above 30 ppm. However, Daphnia magna swimming velocities remained unaltered in the presence of nitrate concentrations below 100 ppm.

Temperature-driven response reversibility and short-term quasi-acclimation of Daphnia magna.

Analysing the effect water temperature has on Daphnia magna is essential in anticipating the impact climate change will have on this freshwater zooplanktonic keystone species. While many authors have followed this line of research, few have covered an extensive temperature range or complex temperature change scenarios. Global warming is mostly associated with increased extreme temperature events, such as heat waves, as well as earlier and more intense thermal stratification. Both of these events may directly influence D. magna fitness, especially in those populations performing diel vertical migration (DVM). We analysed the effect water temperatures, ranging from 11 to 29°C, have on the filtration capacity (FC) of D. magna, to anticipate the effects of acclimation, temperature change rate (TCR) and potential reversibility of responses to such conditions. Results show that sudden temperature changes have an immediate negative impact on the FC of D. magna and is more severe at higher temperatures and higher TCRs. However, D. magna individuals have shown themselves to be capable of quasi-acclimating to temperatures ranging from 11 to 25°C in around a week and achieving much higher FCs; albeit never reaching the optimal FC achieved at 20°C. That said, 29°C is lethal for D. magna individuals within approximately five days. Finally, non-optimal temperature acclimated individuals can recover maximal FC within 2-4 days of the optimal long-term acclimation temperature (20°C) being re-established, thus proving temperature responses to be reversible.

Local hydrodynamics at edges of marine canopies under oscillatory flows.

Canopy fragmentation increases both spatial heterogeneity and patch edges which, in turn, is then likely to modify the local hydrodynamics in the canopy. The orientation of the edge versus the wave and current field is also expected to play an important role in determining wave attenuation and sheltering at the edge of a canopy. We investigated the effect a longitudinal edge (i.e. with its main axis aligned to wave direction) of a simulated canopy has on local edge hydrodynamics. The effect that both canopy density and flexibility have on the hydrodynamics was studied. Flexible plants reduced the wave velocity and the turbulent kinetic energy with distance into the canopy and this attenuation increased as the density of the canopy increased. Compared to flexible plants, an edge of rigid plants produced a higher wave velocity attenuation coupled with an increase in the turbulent kinetic energy with distance into the canopy despite having the same canopy density. This greater wave attenuation at the edge coincided with the shifting of the associated mean current that, in turn, produced an increase in the turbulent kinetic energy at the edge in the canopy. The effect was accentuated when the canopy density increased. The wave velocity attenuation was a linear function of the canopy cover. While flexible plants reduced the turbulent kinetic energy following a linear function of the canopy cover, rigid canopies increased the turbulent kinetic energy following a linear function of the canopy cover. In the case of the flexible vegetation, the lengths of both the inner and outer canopy boundary layers increased as the canopy cover increased.

Strategies for reducing the risk of transfusion-transmitted leishmaniasis in an area endemic for Leishmania infantum: a patient- and donor-targeted approach.

BACKGROUND: In the Balearic Islands, as in other areas of the Mediterranean basin, there is a significant proportion of asymptomatic Leishmania (L.) infantum-infected blood donors, who may represent an important threat to transfusion safety. The Balearic Islands blood bank, located in an area endemic for L. infantum, carried out a study of donors and patients to investigate the impact of this infectious disease on blood safety in the region. MATERIALS AND METHODS: Twenty asymptomatic Leishmania-infected blood donors were followed-up between 2008 and 2011 to investigate the evolution of Leishmania infection in asymptomatic carriers. Their blood was periodically tested for anti-Leishmania antibodies by western blot and for Leishmania DNA by quantitative polymerase chain reaction (qPCR). Additionally, the prevalence of L. infantum infection was investigated in a group of 68 multiply transfused patients to ascertain the risk of transfusion-transmitted leishmaniasis (TTL) in the region, taking into account regular blood component production practices such as pre-storage leucodepletion and pathogen reduction technology. RESULTS: All 20 donors remained asymptomatic over the study period (2008-2011). Most donors had repeatedly positive qPCR results, either persistently or intermittently, but showed no symptoms of Leishmaniasis. Levels of parasitaemia were remarkably low in asymptomatic donors, with values ≤1 parasite/mL. Despite multiple transfusions received over 15 years, no transfused patient studied was infected with L. infantum. DISCUSSION: L. infantum-infected donors can remain asymptomatic for at least 3 years. In our region, no cases of TTL were detected, despite an active search in multiply transfused patients. This seems to be related to two independent variables: (i) a low concentration of the parasite in the peripheral blood of asymptomatic carriers and (ii) the application of methods with proven efficacy against TTL, such as leucodepletion and pathogen reduction technology.

Daphnia magna filtration efficiency and mobility in laminar to turbulent flows.

Daphnia are filter feeder organisms that prey on small particles suspended in the water column. Since Daphnia individuals can feed on wastewater particles, they have been recently proposed as potential organisms for tertiary wastewater treatment. However, analysing the effects of hydrodynamics on Daphnia individuals has scarcely been studied. This study focuses then, on quantifying the filtration and swimming velocities of D. magna individuals under different hydrodynamic conditions. Both D. magna filtration and movement responded differently if the flow was laminar or if it was turbulent. In a laminar-dominated flow regime Daphnia filtration was enhanced up to 2.6 times that of a steady flow, but in the turbulent-dominated flow regime D. magna filtration was inhibited. In the laminar flow regime D. magna individuals moved freely in all directions, whereas in the turbulent flow regime they were driven by the streamlines of the flow. A model based on Daphnia-particle encountering revealed that the filtration efficiency in the laminar regime was driven by the length of the D. magna individuals and the shear rate imposed by the system.

The potential predictive value of MRI and PET-CT in mucinous and nonmucinous rectal cancer to identify patients at high risk of metastatic disease.

OBJECTIVE: To correlate imaging parameters from baseline MRI diffusion-weighted imaging (DWI) and fludeoxyglucose (FDG) positron emission tomography (PET)-CT with synchronous and metachronous metastases in mucinous carcinoma (MC) and non-mucinous carcinoma (NMC) rectal cancer. METHODS: 111 patients with extraperitoneal locally advanced rectal cancer, who underwent pelvic MRI, DWI and FDG PET-CT, were stratified into MC (n = 23) and NMC (n = 88). We correlated adverse morphologic features on MRI [mT4, mesorectal fascia involvement, extramural venous invasion (mEMVI), mN2] and quantitative imaging parameters [minimum apparent diffusion coefficient (ADCmin), maximum standardized uptake value, total lesion glycolysis, metabolic tumour volume, T2 weighted and DWI tumour volumes] with the presence of metastatic disease. All patients underwent pre-operative chemoradiation therapy (CRT); 100/111 patients underwent surgery after CRT and were classified as pathological complete response (PCR) and no PCR [tumour regression grade (TRG)1 vs TRG2-5] and as ypN0 and ypN1-2. Median follow-up time was 48 months. Metastases were confirmed on FDG PET-CT and contrast-enhanced multidetector CT. RESULTS: The percentage of mucin measured by MRI correlates with that quantified by histology. On multivariate analysis, the synchronous metastases were correlated with mEMVI [odds ratio (OR) = 21.48, p < 0.01] and low ADCmin (OR = 0.04, p = 0.038) in NMC. The difference of metachronous recurrence between the MC group (10-90% mucin) and NMC group was significant (p < 0.01) (OR = 21.67, 95% confidence interval 3.8-120.5). Metachronous metastases were correlated with ypN2 (OR = 8.24, p = 0.01) in MC and in NMC. In NMC, mEMVI correlated with no PCR (p = 0.018) and ypN2 (p < 0.01). CONCLUSION: mEMVI could identify patients with NMC, who are at high risk of synchronous metastases. The MC group is at a high risk of developing metachronous metastases. Advances in knowledge: Patients at high risk of metastases are more likely to benefit from more aggressive neoadjuvant therapy.