Biotransformation of 4-nitrophenol by co-immobilized Geobacter sulfurreducens and anthraquinone-2-sulfonate in barium alginate beads.

Geobacter sulfurreducens and anthraquinone-2-sulfonate (AQS) were used suspended and immobilized in barium alginate during the biotransformation of 4-nitrophenol (4-NP). The assays were conducted at different concentrations of 4-NP (50-400 mg/L) and AQS, either in suspended (0-400 µM) or immobilized form (0 or 760 µM), and under different pH values (5-9). G. sulfurreducens showed low capacity to reduce 4-NP in absence of AQS, especially at the highest concentrations of the contaminant. AQS improved the reduction rates from 0.0086 h-1, without AQS, to 0.149 h-1 at 400 µM AQS, which represent an increment of 17.3-fold. The co-immobilization of AQS and G. sulfurreducens in barium alginate beads (AQSi-Gi) increased the reduction rates up to 4.8- and 7.2-fold, compared to incubations with G. sulfurreducens in suspended and immobilized form, but in absence of AQS. AQSi-Gi provides to G. sulfurreducens a barrier against the possibly inhibiting effects of 4-NP.

Role of methanogenesis on the biotransformation of organic micropollutants during anaerobic digestion.

Several studies showed that some organic micropollutants (OMPs) are biotransformed during anaerobic digestion (AD). Yet, most of them aim at reporting removal efficiencies instead of understanding the biotransformation process. Indeed, how each of the main AD stages (i.e., hydrolysis, acidogenesis, and methanogenesis) contribute to OMP biotransformation remains unknown. This study focuses on investigating the role of methanogenesis, the most characteristic step of AD, to OMP removal. More specifically, the sorption and the biotransformation of 20 OMPs by methanogenic biomass were analyzed determining their concentrations in both liquid and solid phases. Sorption onto methanogenic biomass displayed a similar behavior as reported for digested sludge. Most of the OMPs were biotransformed to a medium extent (35-70%) and only sulfamethoxazole was completely removed. Comparing these results with those reported for the complete AD process, methanogenesis was proven to play a key role, accounting for more than 50% of the OMP biotransformation (except for roxithromycin) during AD. An increase in the organic loading rate from 1 to 2gCOD/Ld, typical loads employed in sewage sludge anaerobic digesters, did not exert a clear cometabolic effect on the OMPs biotransformation. It is hypothesized that biotransformation occurs in both liquid and solid phases because no link between the partition coefficient (Kd) and the overall biotransformation efficiency was found. These findings allow a better understanding of the OMPs fate under anaerobic conditions, which is necessary to design efficient biological mitigation strategies.

Biodegradation of p-cresol and sulfide removal by a marine-denitrifying consortium.

The simultaneous removal of sulfide and p-cresol was carried out by using a marine-denitrifying consortium collected in the coastal zone of Sonora, Mexico. Different experimental conditions were used to evaluate the capacity of the consortium to simultaneously eliminate nitrate, sulfide, and p-cresol. For instance, the first set of assays was conducted at different sulfide concentrations (20, 50, and 100 mg S(2À) L(À1) ), with a fixed concentration of p-cresol (45 mg C L(À1) ). The second set of assays was developed at different concentrations of p-cresol (45, 75, and 100 mg C L(-1) ), in the presence of 20 mg S(2À) L(À1) . In all cases, the concentration of nitrate was stoichiometrically added for the complete oxidization of the substrates. The results showed removal efficiencies up to 92% for p-cresol and nitrate at 20 and 50 mg S(2À) L(À1) ; whereas at 100 mg S(2À) L(À1) removal efficiencies were 77% and 59% for p-cresol and nitrate, respectively. On the other hand, sulfide (20 mg L(À1) ) was completely removed under different concentrations of p-cresol tested, with a partial accumulation of nitrite according to the increment of p-cresol concentration. The results obtained indicate that the marine consortium was able to simultaneously remove the pollutants studied.