ABSTRACT
Background: There has been an increase in the prevalence of hypersensitivity to Anisakis simplex. There are fish parasites other than Anisakis simplex whose allergenicity has not yet been studied. Objective: To assess IgE hypersensitivity caused by fish parasite allergens in patients with gastro-allergic symptoms after consumption of fish, shellfish or cephalopods, compared with healthy subjects, pollen allergic individuals and children with digestive symptoms after eating marine food. Methods: We carried out in vivo tests (skin prick) and in vitro tests (specific IgE determination, Western blot) and component resolved diagnostics (CRD) using microarray analysis in all patients. Results: CRD better detected sensitisation to allergens from marine parasites than skin prick tests and determination of specific IgE by CAP. Sensitisation to Gymnorhynchus gigas was detected in 26% of patients measured by skin prick tests and 36% measured by IgE. Conclusions: The prevalence of hypersensitivity to marine parasite allergens other than Anisakis simplex should be studied, and the most appropriate technique for this is CRD (AU)
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Subject(s)
Humans , Anisakis/pathogenicity , Food Hypersensitivity/diagnosis , Anaphylaxis/diagnosis , Parasitic Diseases, Animal/diagnosis , Fish Diseases/parasitology , Skin Tests , Allergens/analysisABSTRACT
BACKGROUND: There has been an increase in the prevalence of hypersensitivity to Anisakis simplex. There are fish parasites other than Anisakis simplex whose allergenicity has not yet been studied. OBJECTIVE: To assess IgE hypersensitivity caused by fish parasite allergens in patients with gastro-allergic symptoms after consumption of fish, shellfish or cephalopods, compared with healthy subjects, pollen allergic individuals and children with digestive symptoms after eating marine food. METHODS: We carried out in vivo tests (skin prick) and in vitro tests (specific IgE determination, Western blot) and component resolved diagnostics (CRD) using microarray analysis in all patients. RESULTS: CRD better detected sensitisation to allergens from marine parasites than skin prick tests and determination of specific IgE by CAP. Sensitisation to Gymnorhynchus gigas was detected in 26% of patients measured by skin prick tests and 36% measured by IgE. CONCLUSIONS: The prevalence of hypersensitivity to marine parasite allergens other than Anisakis simplex should be studied, and the most appropriate technique for this is CRD.
Subject(s)
Antibodies, Helminth/blood , Fish Diseases/parasitology , Fish Products/parasitology , Hypersensitivity/diagnosis , Immunoglobulin E/blood , Adolescent , Adult , Allergens/immunology , Animals , Anisakis/immunology , Antigens, Helminth/immunology , Cestoda/immunology , Child , Eating , Female , Humans , Hypersensitivity/epidemiology , Immunization/statistics & numerical data , Male , Pathology, Molecular , Prevalence , Skin Tests , Young AdultABSTRACT
El propósito de este estudio experimental fue comparar los cambios clínicos e histopatológicos que suceden en recubrimientos pulpares directos. Se analizó el comportamiento de la pulpa dentaria de dientes en pacientes después de realizar la protección directa de la pulpa con Plasma rico en plaquetas. Se realizaron cavidades clase I, las cuales fueron preparadas y posteriormente se expuso el tejido pulpar. Estas fueron llevadas a cabo en 12 piezas dentarias de pacientes. Los grupos fueron divididos: 5 con Plasma rico en plaquetas (liquido), 5 con Plasma rico en plaquetas (gel), 2 con Hidróxido de calcio y 2 piezas dentales sanas (grupo control de procedimiento). Después de un periodo entre 47 y 76 días post tratamiento los dientes fueron extraídos, los especimenes fueron preparados para análisis histológico para verificar la respuesta del complejo dentino-pulpar. La mayoría de los casos presentó inflamación crónica moderada, y los efectos sobre el complejo dentino - pulpar de las piezas a los que se aplicó PRP (gel), se encontraron histológicamente en proceso de regeneración tisular y la manutención de la vitalidad del tejido conjuntivo pulpar subyacente.
