ABSTRACT
Samples of diarrhoeic shellfish poison (DSP) mussels from several parts of the Italian Adriatic coastline were extracted and tested according to a number of different methods presently available, i.e. Yasumoto's mouse biotest, Kat's biotest, the ELISA test and the HPLC method. Results were compared for toxic levels detected in each sample. While a common qualitative result (toxic/non-toxic) was given by all the methods, no clear quantitative agreement was found. The differences between methods and consequent lack of agreement in results are discussed.
Subject(s)
Bivalvia/chemistry , Ethers, Cyclic/analysis , Marine Toxins/analysis , Animals , Biological Assay , Chromatography, High Pressure Liquid , Diarrhea/etiology , Enzyme-Linked Immunosorbent Assay , Ethers, Cyclic/toxicity , Female , Marine Toxins/toxicity , Mice , Okadaic Acid , RatsABSTRACT
Algal extracts of Anabaena planctonica from Lake Mulargia in Italy were tested for toxins by mouse bioassay, the Microtox system, and GC-MS and HPLC chromatography. Anatoxin-a was identified by GC-MS after derivatization with pentafluorobenzyl bromide. Hepatotoxins were also present and are perhaps related to the microcystins present in other species of Anabaena.
