Subject(s)
Asthma/blood , Immunotherapy/methods , Ribonucleases/blood , Adolescent , Adult , Antibodies/blood , Asthma/immunology , Asthma/therapy , Biomarkers/blood , Blood Proteins , Eosinophil Granule Proteins , Female , Humans , Immunoglobulin E/immunology , Immunoglobulin G/immunology , Male , Middle Aged , Time FactorsABSTRACT
It was shown that intake of carnosine in a dose of 50-100 mg/kg of body weight before X-ray irradiation resulted in an increase of the survival of experimental mice. The protective effect of carnosine was manifested, when it was injected either before or after irradiation, but the effect was more pronounced in the case of shortening time between irradiation and injection. An enhancement of colony forming index of bound cells in spleen was also observed simultaneously with protective action of carnosine. These effects are supposed to be the result of immunomodulating activity of carnosine.
Subject(s)
Carnosine/pharmacology , Hematopoietic Stem Cells/drug effects , Radiation-Protective Agents , Animals , Colony-Forming Units Assay , Hematopoietic Stem Cells/radiation effects , Mice , Time FactorsABSTRACT
The effect of osmotic shock on human lymphocytes receptors is investigated. The patients are shown to be varied in terms of osmotic stability of lymphocytes, although under osmotic shock the decrease of cell share with definite immunological markers is observed in all cases. It is characteristic of all T-lymphocyte populations. The incubation of non-diluted blood with carnosin preserves largely the investigated receptors at osmotic shock. It is suggested that described modifications in lymphocyte receptor system connect with immunocorrective effect of the hypoosmotic blood.
Subject(s)
T-Lymphocytes/immunology , Carnosine/pharmacology , Humans , Immunologic Deficiency Syndromes/immunology , Leukocyte Count/drug effects , Osmotic Pressure/drug effects , Receptors, Immunologic/drug effects , Receptors, Immunologic/immunology , Rosette Formation , T-Lymphocytes/drug effects , Theophylline/pharmacologyABSTRACT
An efficient method for the treatment of drug allergy was proposed and practically implemented. The method consists in autologous venous blood being lyzed with sterile bidistilled water at a ratio of 5:1 and injected subcutaneously and partially intradermally into the reflexogenic zones of the back 2-3 cm from the spinal column. During the first week of treatment, increasing doses were injected (3 to 10 ml), whereas during the second week the doses decreased from 10 to 3 ml. Following treatment the patients felt better and featured enhanced working ability as well as markedly declined susceptibility to common colds. The most informative immunological indicators were chosen to evaluate the efficiency of therapy.
Subject(s)
Blood Transfusion, Autologous , Drug Hypersensitivity/therapy , Immunization, Passive/methods , Adolescent , Adult , Child , Child, Preschool , Female , Humans , Immunoglobulins/analysis , Male , Middle Aged , T-Lymphocytes/immunology , T-Lymphocytes/pathologyABSTRACT
A study of immunomodulating action of mouse alpha/beta interferon (IFN) was performed in conditions of congenital and experimental thymus-dependent immune deficiency. The action of IFN was assessed in vivo, with IFN injected intraperitoneally. It was shown that IFN did not change killer effect of lymphocytes in the reaction of stem cell inactivation in "nude" mice, but enhanced antibody and rosette formation in the spleen of these animals. In addition, IFN did not change these parameters in the spleen of HRS mice with abnormal differentiation of T lymphocytes, but enhanced antibody and rosette formation in the spleen of mice, thymectomized in old age. It is concluded that the normal function of T-system is essential for the action of IFN.
Subject(s)
Adjuvants, Immunologic , Immunologic Deficiency Syndromes/congenital , Immunologic Deficiency Syndromes/therapy , Interferon Type I/therapeutic use , Thymus Gland/immunology , Animals , Antibody Formation/drug effects , Hematopoietic Stem Cells/drug effects , Hematopoietic Stem Cells/immunology , Immunologic Deficiency Syndromes/immunology , Male , Mice , Mice, Inbred Strains , Rosette Formation , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , Thymus Gland/drug effectsABSTRACT
The analysis of the immune status of patients under neurodermatitis after autologous blood treatment has been performed. It has been found that positive therapeutic effect correlates with a number of immunologic parameters: the lysozyme content raises, the metabolic activity of neutrophilic cells increases, a number of T-lymphocytes grows to a certain degree, the balance of T-lymphocyte subpopulation normalizes.
Subject(s)
Blood Transfusion, Autologous , Immune System/immunology , Humans , Immunity, Cellular , Immunoglobulins/analysis , Neurodermatitis/immunology , Neurodermatitis/therapy , Osmolar Concentration , PhagocytosisSubject(s)
Hematopoietic Stem Cells , Histocompatibility , Lymphocyte Transfusion , Animals , Bone Marrow Cells , Bone Marrow Transplantation , Cells, Cultured , Female , Male , Mice , Mice, Inbred AKR , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Inbred DBA , Transplantation, HomologousABSTRACT
Inoculation of a mixture of bone marrow cells with allogeneic lymphocytes into irradiated mice of inbred strains or into F1 hybrids results in the depression of bone marrow cell proliferation in the spleen of the recipient: the effect of inactivation of nonsyngeneic stem cells. The inactivation of stem cells by allogeneic lymphocytes can be detected in all tested combinations of mice strains - donors of lymphocytes and bone marrow cells and mice - recipients but the degree of inactivation differs and depends on the genotype of cell donors rather than on the genotype of the recipient. Lymphocytes of some mice strains (haplotypes H-2k and H-2a) are more active killers of bone marrow cells as compares with lymphocytes of other strains (hyplotypes H-2b and H-2d). Probably, the degree of stem cells inactivation by lymphocytes depends on the differences of their histocompatibility in H-2 system.
