ABSTRACT
Continuous monitoring of ammonia removal by microbial complexes and observation of their morphology were carried out using a microdevice. Consumption of NH(4)(+) ions by active sludge could clearly be recorded over 48 h. Aggregation of the sludge could be observed in parallel by using confocal reflection microscopy.
Subject(s)
Ammonia/metabolism , Microbiological Techniques/methods , Sewage/microbiology , Microscopy, Confocal/methodsABSTRACT
The feasibility of a method to monitor biofilm development non-destructively in a microfluidic device was addressed. Here, we report that biofilm growth could be non-destructively monitored by an image analysis technique based on modification of confocal reflection microscopy.
Subject(s)
Bacterial Load/methods , Biofilms/growth & development , Image Interpretation, Computer-Assisted/methods , Microfluidic Analytical Techniques/methods , Microscopy, Confocal/methods , Streptococcus mutans/cytology , Streptococcus mutans/growth & development , Bacterial Load/instrumentation , Bioreactors , Cell Culture Techniques , Microfluidic Analytical Techniques/instrumentationABSTRACT
The feasibility of a method to nondestructively measure planktonic bacterial growth in a microfluidic device was addressed. Here, we report that the growth of Pseudomonas aeruginosa in a microfluidic device could be measured by a three-dimensional image analysis technique based on confocal reflection microscopy in a time-course.
