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1.
Science ; 331(6021): 1154-9, 2011 Mar 04.
Article in English | MEDLINE | ID: mdl-21385708

ABSTRACT

Morphogens, such as Decapentaplegic (Dpp) in the fly imaginal discs, form graded concentration profiles that control patterning and growth of developing organs. In the imaginal discs, proliferative growth is homogeneous in space, posing the conundrum of how morphogen concentration gradients could control position-independent growth. To understand the mechanism of proliferation control by the Dpp gradient, we quantified Dpp concentration and signaling levels during wing disc growth. Both Dpp concentration and signaling gradients scale with tissue size during development. On average, cells divide when Dpp signaling levels have increased by 50%. Our observations are consistent with a growth control mechanism based on temporal changes of cellular morphogen signaling levels. For a scaling gradient, this mechanism generates position-independent growth rates.


Subject(s)
Cell Proliferation , Drosophila Proteins/metabolism , Drosophila melanogaster/growth & development , Drosophila melanogaster/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Signal Transduction , Wings, Animal/growth & development , Wings, Animal/metabolism , Animals , Cell Cycle , Computer Simulation , Drosophila melanogaster/cytology , Drosophila melanogaster/genetics , Models, Biological , Morphogenesis , Mutation , Wings, Animal/anatomy & histology , Wings, Animal/cytology
2.
EMBO J ; 20(4): 812-8, 2001 Feb 15.
Article in English | MEDLINE | ID: mdl-11179225

ABSTRACT

A transgene inserted in euchromatin exhibits mosaic expression when targeted by a fusion protein made of the DNA-binding domain of GAL4 and the heterochromatin-associated protein HP1. The silencing responds to the loss of a dose of the dominant modifiers of position-effect variegation Su(var)3-7 and Su(var)2-5, the locus encoding HP1. The genomic environs of the insertion site at 87C1 comprise the dispersed repetitive elements micropia and alphagamma. In the presence of the GAL4-HP1 chimera, the polytene chromosomes of this line form loops between the insertion site of the transgene and six other sections of chromosome 3R, as well as, rarely, with pericentric and telomeric heterochromatin. In contrast to the insertion site of the transgene at 87C, the six loop-forming sites in the euchromatic arm were each previously described as intercalary heterochromatin. Moreover, GAL4-HP1 tethering on one homologue trans-inactivates the reporter on the other. HP1, probably together with other partners, could thus facilitate the coalescence of dispersed middle repetitive sequences, and organize the heterochromatic structure responsible for the variegated silencing of nearby euchromatic genes.


Subject(s)
Chromosomal Proteins, Non-Histone/physiology , Chromosomes , Drosophila/genetics , Gene Silencing , Animals , Base Sequence , Chromobox Protein Homolog 5 , DNA Primers , Genes, Reporter , Polymerase Chain Reaction , Recombinant Fusion Proteins/metabolism , Repetitive Sequences, Nucleic Acid , Transgenes
3.
Chromosoma ; 109(7): 453-9, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11151674

ABSTRACT

We have constructed a new reporter transgene, Winkelried, equipped with a synthetic binding site for the yeast GAL4 transcriptional activator. The binding site is inserted between the white and lacZ reporter genes, and is flanked by FRT sequences. These elements allow excision of the GAL4 binding site by crossing the transgenic line with an FLP recombinase producing strain. We have generated by X-ray irradiation two independent chromosomal rearrangements, Heidi and Tell, relocating Winkelried next to pericentromeric heterochromatin. These rearrangements induce variegation of both white and lacZ. Variegation of Winkelried in the rearranged transgenic lines responds to the loss and excess of doses of the dominant suppressors of position-effect variegation (PEV) Su(var)3-7 and Su(var)2-5. Winkelried therefore constitutes a unique tool to test the effect on variegation in cis of any factor fused to the GAL4 DNA binding domain. Indeed, a chimeric protein, made of the DNA binding site of GAL4 and of HP1, the modifier of PEV encoded by Su(var)2-5, is shown to enhance variegation of Heidi and Tell. Excision of the binding sites for GAL4 in the variegating rearrangements Heidi and Tell abolishes the modifier effect of the GAL4-HP1 chimera. Therefore, in the Heidi and Tell rearrangements, enhancement of position-effect variegation depends strictly both on the concentration of GAL4-HP1 and on the presence of its binding site in the vicinity of the reporter genes.


Subject(s)
DNA-Binding Proteins , Fungal Proteins/metabolism , Gene Silencing , Heterochromatin/metabolism , Nuclear Proteins , Recombinant Fusion Proteins/metabolism , Saccharomyces cerevisiae Proteins , Transcription Factors/metabolism , Base Sequence , Binding Sites , DNA Primers , Fungal Proteins/genetics , Hepatocyte Nuclear Factor 1 , Hepatocyte Nuclear Factor 1-beta , Phenotype , Transcription Factors/genetics , Transgenes
4.
Development ; 122(6): 1949-56, 1996 Jun.
Article in English | MEDLINE | ID: mdl-8674433

ABSTRACT

A dominant mutation due to the insertion of a P-element at 93E on the third chromosome of Drosophila melanogaster enhances position-effect variegation. The corresponding gene was cloned by transposon tagging and the sequence of the transcript revealed that it corresponds to the gene encoding the transcriptional activator and cell cycle regulator dE2F. The transposon-tagged allele is homozygous viable, and the insertion of the transposon in an intron correlates with a strong reduction in the amount of transcript. A homozygous lethal null allele was found to behave as a strong enhancer when heterozygous. Overexpression of the gene in transgenic flies has the opposite effect of suppressing variegation. A link is established here, and discussed, between the dose of a transcriptional activator, which controls the cell cycle, and epigenetic silencing of chromosomal domains in Drosophila.


Subject(s)
Carrier Proteins , Cell Cycle Proteins/genetics , DNA-Binding Proteins , Drosophila Proteins , Drosophila melanogaster/genetics , Eye Color/genetics , Transcription Factors/genetics , Amino Acid Sequence , Animals , Base Sequence , Cell Cycle Proteins/metabolism , Cloning, Molecular , DNA , Drosophila melanogaster/embryology , E2F Transcription Factors , Enhancer Elements, Genetic , Female , Gene Dosage , Gene Expression Regulation , Insect Hormones , Male , Molecular Sequence Data , Mutation , RNA, Messenger/metabolism , Retinoblastoma-Binding Protein 1 , Trans-Activators/genetics , Trans-Activators/metabolism , Transcription Factors/metabolism
5.
Nucleic Acids Res ; 22(21): 4386-94, 1994 Oct 25.
Article in English | MEDLINE | ID: mdl-7971269

ABSTRACT

Two minimal scaffold-associated regions (SARs) from Drosophila were tested in stably transformed cells for their effects on the expression of reporter genes. The expression of genes bounded by two SARs is consistently stimulated by about 20- to 40-fold, if the average of a pool of cell transformants is analyzed. However, analysis of individual, stable cell transformants demonstrates that flanking SAR elements do not confer position-independent expression on the reporter gene and that the extent of position-dependent variegation is similarly large with or without the flanking SAR elements. The SAR stimulation of expression is observed in stable but not in transiently transfected cell lines. The Drosophila scs and scs' boundary elements, which do not bind to the nuclear matrix in vitro, are only about one-tenth as active as SARs in stimulating expression in stable transformants. Interestingly, the SAR stimulatory effect can be blocked by a fragment containing CpG islands (approximately 70% GC), if positioned between the SAR and the enhancer. In contrast, when inserted in the same position, control fragments, such as the scs/scs' elements, do not interfere with SAR function.


Subject(s)
Chloramphenicol O-Acetyltransferase/genetics , DNA/chemistry , Gene Expression , Animals , Base Composition , Drosophila/genetics , Enhancer Elements, Genetic , Genes, Reporter , HSP70 Heat-Shock Proteins/genetics , HeLa Cells , Histones/genetics , Humans , L Cells/metabolism , Mice , Promoter Regions, Genetic , Saccharomyces cerevisiae/genetics , Simian virus 40/genetics , Transcription, Genetic , Transfection
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