ABSTRACT
Administration of Surfagon, a gonadotropin-releasing hormone analogue, in doses of 0.1 and 5.0 microg/kg before emotional nociceptive stress increased lymphocyte migration from the thymus, decreased the volume of lymphoid tissue in the spleen and thymus, reduced the width of the zona fasciculata and increased the width of the zona glomerulosa in the adrenal cortex of male CBA mice. These effects of the peptide persisted in castrated animals. Surfagon prevented stress-induced activation of the adrenal glands and accidental transformation of the thymus and spleen in castrated animals.
Subject(s)
Adrenal Glands/drug effects , Gonadotropin-Releasing Hormone/analogs & derivatives , Spleen/drug effects , Stress, Psychological/physiopathology , Thymus Gland/drug effects , Adrenal Glands/cytology , Animals , Cell Movement/drug effects , Dose-Response Relationship, Drug , Gonadotropin-Releasing Hormone/administration & dosage , Gonadotropin-Releasing Hormone/pharmacology , Male , Mice , Mice, Inbred CBA , Spleen/cytology , Thymus Gland/cytologyABSTRACT
Intraperitoneal treatment with an analogue of gonadotropin-releasing hormone in doses of 0.004-450 microg/kg produced an analgesic effect on male mice in the hot plate test. Castration significantly elevated the nociceptive thresholds. In castrated mice the effects of the test peptide were less pronounced and had an algesic nature. Our results indicate that these effects depend on functional activity of the hypothalamic-pituitary-gonadal axis.
Subject(s)
Gonadotropin-Releasing Hormone/analogs & derivatives , Nociceptors/drug effects , Analgesics/pharmacology , Animals , Gonadotropin-Releasing Hormone/pharmacology , Hot Temperature , Hypothalamo-Hypophyseal System/drug effects , Hypothalamo-Hypophyseal System/physiology , Male , Mice , Mice, Inbred BALB C , Nociceptors/physiology , Orchiectomy , Pain Measurement , Pain Threshold/drug effects , Pituitary-Adrenal System/drug effects , Pituitary-Adrenal System/physiology , Testis/physiologyABSTRACT
The mitotic index of hepatocytes remained unchanged after 10 intraperitoneal injections of DSLET and ACTH(4-10) in doses of 0.5, 1.5, and 5 microg/kg, but increased after injection of these substances in doses of 50 and 150 microg/kg. DSLET in doses of 5, 50, and 150 microg/kg decreased the number of antibody-producing cells in the spleen. ACTH(4-10) possessed immunosuppressive activity not only in these doses, but also in a dose of 1.5 microg/kg. As differentiated from mitotic activity of hepatocytes, the degree of immunosuppression increased with increasing the dose of test peptides.
Subject(s)
Adrenocorticotropic Hormone/pharmacology , Antibodies/metabolism , Enkephalin, Leucine/analogs & derivatives , Enkephalin, Leucine/pharmacology , Hepatocytes , Mitosis/physiology , Peptide Fragments/pharmacology , Analgesics/pharmacology , Animals , Dose-Response Relationship, Drug , Hepatocytes/drug effects , Hepatocytes/physiology , Mice , Mice, Inbred CBA , Nootropic Agents/pharmacologyABSTRACT
Ten intraperitoneal injections of tripeptide Gly-His-Lys in doses of 1.5, 5, 50, 150, and 450 mg/kg stimulated mitotic activity of hepatocytes and dose-dependently suppressed immune reactivity (number of antibody-producing cells and delayed-type hypersensitivity reaction).
Subject(s)
Hepatocytes/drug effects , Immunosuppressive Agents/pharmacology , Oligopeptides/pharmacology , Amino Acid Sequence , Animals , Antibody Formation , Dose-Response Relationship, Immunologic , Hepatocytes/metabolism , Hypersensitivity, Delayed/prevention & control , Immunization, Passive , Immunosuppressive Agents/chemistry , Mice , Mice, Inbred CBA , Mitotic Index , Oligopeptides/chemistry , Rats , Rats, WistarABSTRACT
Surfagon, a synthetic analog of gonadotropin-releasing hormone injected before emotional painful stress dose-dependently changed the number of antibody-producing cells in rats and phagocytic and functional activities of neutrophils in mice. In castrated animals this peptide increased all studied parameters. This suggests that sex steroids are not involved in the realization of these effects.
