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1.
A simplified method for measuring scheduled and unscheduled DNA synthesis in human fibroblasts.
Agrelo, C E; Severn, B J.
Toxicology ; 21(2): 151-8, 1981.
Article in English | MEDLINE | ID: mdl-7281203

ABSTRACT

A simplified method for the rapid measurement of scheduled and unscheduled DNA synthesis in cultures of human fibroblasts is described. The use of cultures in which DNA synthesis is blocked by hydroxyurea (HU) together with those which are not blocked allows an estimation of the effect of chemicals on both types of DNA synthesis. This facilitates the interpretation of results and the selection of an optimum range of doses. Some results obtained with carcinogens and non-carcinogens are presented of the compounds so far tested. The methodology here described compares well in its validity with other more difficult and time consuming methods.


Subject(s)
Carcinogens/pharmacology , DNA/biosynthesis , DNA Repair/drug effects , Drug Evaluation, Preclinical/methods , Fibroblasts/drug effects , Humans , Hydroxyurea/pharmacology , Thymidine/metabolism
2.
The effect of prolonged sodium phenobarbitone treatment on hepatic xenobiotic metabolism and the urinary excretion of metabolites of the D-glucuronic acid pathway in the rat.
Lake, B G; Longland, R C; Harris, R A; Severn, B J; Gangolli, S D.
Biochem Pharmacol ; 27(19): 2357-61, 1978.
Article in English | MEDLINE | ID: mdl-728187

Subject(s)
Glucuronates/metabolism , Liver/metabolism , Pharmaceutical Preparations/metabolism , Phenobarbital/pharmacology , Animals , Diet , Glucuronates/urine , Hexobarbital/pharmacology , In Vitro Techniques , Liver/drug effects , Liver/enzymology , Male , Mixed Function Oxygenases/metabolism , Rats , Sleep/drug effects , Time Factors
3.
The effect of some foreign compounds on hepatic xenobiotic metabolism and on the urinary excretion of D-glucuronic acid metabolites in the rat.
Lake, B G; Longland, R C; Hodgson, R A; Severn, B J; Gangolli, S D.
Biochem Soc Trans ; 5(1): 308-9, 1977.
Article in English | MEDLINE | ID: mdl-408209

Subject(s)
Carbon Tetrachloride/pharmacology , Coumarins/pharmacology , Cytochrome P-450 Enzyme System/metabolism , Disulfiram/pharmacology , Ethylmorphine-N-Demethylase/metabolism , Glucuronates/urine , Glucuronosyltransferase/metabolism , Microsomes, Liver/enzymology , Oxidoreductases, N-Demethylating/metabolism , Animals , Male , Microsomes, Liver/drug effects , Organ Size , Rats
4.
Studies on the hydrolysis in vitro of phthalate esters by hepatic and intestinal mucosal preparations from various species.
Lake, B G; Phillips, J C; Hodgson, R A; Severn, B J; Gangolli, S D; Lloyd, A G.
Biochem Soc Trans ; 4(4): 654-5, 1976.
Article in English | MEDLINE | ID: mdl-826432

Subject(s)
Intestinal Mucosa/metabolism , Liver/metabolism , Phthalic Acids/metabolism , Animals , Dibutyl Phthalate/metabolism , Diethylhexyl Phthalate/metabolism , Ferrets , Haplorhini , Kinetics , Organ Specificity , Papio , Rats , Species Specificity
5.
The effect of some inducers of hepatic xenobiotic-metabolizing enzymes on the urinary excretion of D-glucuronic acid metabolites in the rat.
Lake, B G; Longland, R C; Hodgson, R A; Severn, B J; Gangolli, S D; Lloyd, A G.
Biochem Soc Trans ; 3(1): 188-91, 1975.
Article in English | MEDLINE | ID: mdl-805065

Subject(s)
Glucuronates/metabolism , Microsomes, Liver/enzymology , Phenobarbital/pharmacology , Aniline Hydroxylase/metabolism , Animals , Cytochrome P-450 Enzyme System/metabolism , Enzyme Induction/drug effects , Ethylmorphine-N-Demethylase/metabolism , Glucuronosyltransferase/metabolism , Hymecromone , Insecticides/pharmacology , Male , Methylcholanthrene/pharmacology , Microsomes, Liver/drug effects , Pregnenolone , Rats , Time Factors , Toluene/pharmacology
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