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1.
Bone ; 46(6): 1661-7, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20156606

ABSTRACT

PURPOSE: The aim of the cross-sectional study was to establish the degree of conformity between 10-year probability of osteoporotic fracture, assessed by FRAX, and using the nomograms, as proposed by Nguyen at al. METHODS: Postmenopausal Polish women (2012) were examined in their mean age of 68.5+/-7.9 years (age range 55-90 years). Fracture probability by FRAX was based on age, BMI, prior fracture, hip fracture in parents, steroid use, rheumatoid arthritis, alcohol use, secondary osteoporosis and T-score for femoral neck BMD. Fracture probability by Nguyen's nomograms was based on age, the number of prior fractures, the number of falls and T-score for femoral neck BMD. RESULTS: The mean conformity rate was 79.1% for any fracture risk (for threshold 20%) and 79.5% for hip fracture (threshold 3%). Any and hip fracture risks were significantly higher for both methods in women with fracture history in comparison to those without fracture and increased with ageing. The influence of prior fracture and ageing was more evident in Nguyen's nomograms. ROC analyses of any fracture risk in FRAX and Nguyen's methods demonstrated the area under curve (AUC) at 0.833 and 0.879, respectively. Similar analyses for hip fracture demonstrated AUCs for FRAX and Nguyen's technique at 0.726 and 0.850, respectively. The AUCs for Nguyen's nomograms were significantly larger than the AUCs for FRAX (p<0.0001). CONCLUSION: The mean conformity for any fracture risk is 79.1% and 79.5% for hip fracture. Nguyen's nomograms seem to be more efficient in fracture risk assessment, especially for hip fractures, due to a higher accuracy of the method. The information on the number of falls during the last year and multiple fractures ought to be incorporated into the method of fracture risk prediction. MINI-ABSTRACT: The degree of conformity was assessed in a group of 2012 women between 10-year FRAX prognosis of fracture and Nguyen et al.'s nomograms. The mean conformity for any fracture risk is 79.1% and 79.5% for hip fracture. Nguyen's nomograms seem to be more efficient in fracture risk assessment due to higher accuracy.


Subject(s)
Fractures, Bone/epidemiology , Age Factors , Aged , Aged, 80 and over , Arthritis, Rheumatoid/epidemiology , Arthritis, Rheumatoid/physiopathology , Bone Density , Female , Femur Neck/injuries , Hip Fractures/epidemiology , Hip Fractures/physiopathology , Humans , Middle Aged , Osteoporosis/epidemiology , Osteoporosis/physiopathology , Osteoporosis, Postmenopausal/epidemiology , Risk Factors
2.
Endocr Regul ; 35(1): 25-30, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11308993

ABSTRACT

OBJECTIVE: To examine the bromodeoxyuridine (BrdU) incorporation into DNA of thyroid follicular cells (TFC) in the remaining thyroid lobe after hemithyroidectomy (hemiTx) in 1, 2, 3 and 4 weeks time after surgery. METHODS: The experiment was performed on male Wistar rats. The Cell Proliferation Kit (Amersham, UK) was used in order to detect the incorporated BrdU. The BrdU incorporation was expressed as a BrdU labeling index (BrdULI; a number of BrdU-immunopositive TFC per 1000 TFC). RESULTS: 1. No statistically significant changes of BrdULI were observed between the particular groups of sham-operated (shamTx)-rats in 1, 2, 3 and 4 weeks time after surgery, and in comparison of each of them to the controls (at time "0"); 2. In the first 2-week period after hemiTx, an increasing effect of that surgical procedure on BrdULI value was observed (the highest BrdULI value was detected 2 weeks after hemiTx); 3. In the third and fourth week after hemiTx, a decrease of BrdULI value was observed, as compared to BrdULI groups (in 1- and 2-week time after hemiTx), and to the controls (at time "0"); 4. An increase of weight of contralateral lobe was shown in 1, 2, 3 and 4 weeks after hemiTx in comparison to thyroid lobe weight in intact rats. CONCLUSIONS: During the first 2 weeks after hemiTx, the thyroid growth in the remaining thyroid lobe seems to ensue by hyperplasia mechanisms. The thyroid growth processes during subsequent 2 weeks (3rd and 4th) could result from other mechanisms - for example, from hypertrophy.


Subject(s)
Bromodeoxyuridine/metabolism , DNA/biosynthesis , Thyroid Gland/metabolism , Thyroidectomy , Animals , Hyperplasia , Male , Organ Size , Rats , Rats, Wistar , Thyroid Gland/pathology
3.
Mutat Res ; 464(2): 239-45, 2000 Jan 24.
Article in English | MEDLINE | ID: mdl-10648910

ABSTRACT

The ability of melatonin to influence paraquat-induced genotoxicity was tested using micronucleated polychromatic erythrocytes as an index of damage in both bone marrow and peripheral blood cells of mice. Melatonin (10 mg/kg) or an equal volume of saline were administered intraperitoneally (ip) to mice 30 min prior to an ip injection of paraquat (20 mg/kgx2), and thereafter at 6-h intervals until the conclusion of the study (72 h). The number of the micronucleated polychromatic erythrocytes increased after paraquat administration both in peripheral blood and bone marrow cells. Melatonin administration to paraquat-treated mice significantly reduced micronuclei formation in both peripheral blood and bone marrow cells; these differences were apparent at 24, 48 and 72 h after paraquat administration. The induction of micronuclei was time-dependent with peak values occurring at 24 and 48 h. The reduction in paraquat-related genotoxicity by melatonin is likely due in part to the antioxidant activity of the indole. We did not observe effects of melatonin over paraquat in paraquat+melatonin groups incubated at 0, 60 and 120 min. Mitomycin C, which was used as a positive control, also caused the expected large rises in micronuclei in both bone marrow and peripheral blood cells at 24, 48 and 72 h after its administration.


Subject(s)
Bone Marrow/drug effects , Herbicides/toxicity , Melatonin/pharmacology , Micronuclei, Chromosome-Defective/drug effects , Paraquat/toxicity , Animals , Bone Marrow/pathology , Chromosome Breakage , DNA/drug effects , Erythroblasts/drug effects , Erythroblasts/pathology , Injections, Intraperitoneal , Male , Melatonin/metabolism , Mice , Micronuclei, Chromosome-Defective/pathology , Micronucleus Tests , Mitomycin/pharmacology , Mutagenicity Tests , Nucleic Acid Synthesis Inhibitors/pharmacology
4.
Toxicol Lett ; 95(2): 103-8, 1998 Mar 31.
Article in English | MEDLINE | ID: mdl-9635413

ABSTRACT

The protection afforded by melatonin against paraquat-induced genotoxicity in both bone marrow and peripheral blood cells of mice was tested using micronuclei as an index of induced chromosomal damage. Melatonin (2 mg/kg) or an equal volume of saline was injected i.p. into mice 30 min prior to the i.p. administration of paraquat (two injections of 15 mg/kg; the paraquat injections were given with a 24 h interval) and thereafter at 6 h intervals to the conclusion of the study (72 h). Using fluorescence microscopy, the number of micronuclei in polychromatic erythrocytes (MN-PCE) per 2000 PCE (1000 PCE/slide) per mouse was counted both in blood and bone marrow, and the ratio of PCE to normochromatic erythrocytes (NCE) (PCE/NCE) was calculated. Paraquat treatment increased the number of MN-PCE at 24, 48, and 72 h, both in peripheral blood and bone marrow cells, while no differences were observed in the PCE/NCE ratio. Melatonin inhibited the paraquat-induced increase in MN-PCE by more than 50% at 48 and 72h. Paraquat toxicity is believed to be due to free radical generation. Since melatonin is known to be an efficient free radical scavenger, it is concluded that melatonin's protection against paraquat-induced genotoxicity is mediated, at least in part, by its free radical scavenging activity.


Subject(s)
DNA Damage/drug effects , Melatonin/pharmacology , Paraquat/toxicity , Animals , Drug Interactions , Free Radicals , Herbicides/toxicity , Male , Mice , Mice, Inbred ICR , Micronucleus Tests
5.
Neurochem Int ; 32(1): 69-75, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9460704

ABSTRACT

Melatonin was recently shown to be a component of the antioxidative defense system of organisms due to its free radical scavenging and antioxidant activities. Pharmacologically, melatonin stimulates the activity of the peroxide detoxifying enzyme glutathione peroxidase in rat brain and in several tissues of chicks. In this report, we studied the endogenous rhythm of two antioxidant enzymes, glutathione peroxidase and glutathione reductase, in five regions (hippocampus, hypothalamus, striatum, cortex and cerebellum) of chick brain and correlated them with physiological blood melatonin concentrations. Glutathione peroxidase exhibited a marked 24 h rhythm with peak activity in each brain region which had acrophases about 8 h after lights off and about 4 h after the serum melatonin peak was detected. Glutathione reductase activity exhibited similar robust rhythms with the peaks occurring roughly 2 h after those of glutathione peroxidase. We suggest that neural glutathione peroxidase increases due to the rise of nocturnal melatonin levels while glutathione reductase activity rises slightly later possibly due to an increase of its substrate, oxidized glutathione. The exposure of chicks to constant light for 6 days eliminated the melatonin rhythm as well as the peaks in both glutathione peroxidase and glutathione reductase activities. These findings suggest that the melatonin rhythm may be related to the nighttime increases in the enzyme activities, although other explanations cannot be excluded.


Subject(s)
Brain/enzymology , Circadian Rhythm , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Light , Animals , Antioxidants , Cerebellum/enzymology , Cerebral Cortex/enzymology , Chickens , Corpus Striatum/enzymology , Hippocampus/enzymology , Hypothalamus/enzymology , Melatonin/blood , Photoperiod
6.
J Appl Physiol (1985) ; 83(2): 354-8, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9262426

ABSTRACT

Hyperbaric oxygen exposure rapidly induces lipid peroxidation and cellular damage in a variety of organs. In this study, we demonstrate that the exposure of rats to 4 atmospheres of 100% oxygen for 90 min is associated with increased levels of lipid peroxidation products [malonaldehyde (MDA) and 4-hydroxyalkenals (4-HDA)] and with changes in the activities of two antioxidative enzymes [glutathione peroxidase (GPX) and glutathione reductase (GR)], as well as in the glutathione status in the lungs and in the brain. Products of lipid peroxidation increased after hyperbaric hyperoxia, both GPX and GR activities were decreased, and levels of total glutathione (reduced+oxidized) and glutathione disulfide (oxidized glutathione) increased in both lung and brain areas (cerebral cortex, hippocampus, hypothalamus, striatum, and cerebellum) but not in liver. When animals were injected with melatonin (10 mg/kg) immediately before the 90-min hyperbaric oxygen exposure, all measurements of oxidative damage were prevented and were similar to those in untreated control animals. Melatonin's actions may be related to a variety of mechanisms, some of which remain to be identified, including its ability to directly scavenge free radicals and its induction of antioxidative enzymes via specific melatonin receptors.


Subject(s)
Brain/drug effects , Brain/metabolism , Hyperbaric Oxygenation , Lung/drug effects , Lung/metabolism , Melatonin/pharmacology , Aldehydes/metabolism , Animals , Glutathione/metabolism , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Lipid Peroxides/metabolism , Liver/metabolism , Male , Malondialdehyde/metabolism , Rats , Rats, Sprague-Dawley
7.
J Pineal Res ; 23(1): 24-31, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9379343

ABSTRACT

While nitric oxide (NO) has been implicated as a mediator of glutamate excitotoxicity after cerebral ischemia/reperfusion, melatonin has been reported to inhibit brain NO production by suppressing nitric oxide synthase. The purpose of the present studies was to determine the effect of exogenous melatonin administration on NO-induced changes during brain ischemia/reperfusion. Indicators of cerebral cortical and cerebellar NO production [nitrite/nitrate levels and cyclic guanosine monophosphate (cGMP)] were used to estimate neural changes after transient bilateral carotid artery ligation followed by reperfusion in adult Mongolian gerbils (Meriones unguiculatus). Results show for the first time that melatonin prevents the increases in NO and cGMP production after transient ischemia/reperfusion in frontal cerebral cortex and cerebellum of Mongolian gerbils. The inhibitory effect of melatonin on NO production and its ability to scavenge free radicals and the peroxynitrite anion may be responsible for the protective effect of melatonin on neuronal structures during transient ischemia followed by reperfusion.


Subject(s)
Brain/metabolism , Cyclic GMP/biosynthesis , Ischemic Attack, Transient/metabolism , Melatonin/pharmacology , Nitric Oxide/biosynthesis , Animals , Brain/drug effects , Carotid Arteries , Cerebellum/metabolism , Cerebral Cortex/metabolism , Constriction , Frontal Lobe/metabolism , Gerbillinae , Male
8.
Br J Pharmacol ; 121(2): 264-70, 1997 May.
Article in English | MEDLINE | ID: mdl-9154336

ABSTRACT

1. Melatonin protection against ethanol-induced gastroduodenal injury was investigated in duodenumligated rats. 2. Melatonin, injected i.p. 30 min before administration of 1 ml of absolute ethanol, given by gavage, significantly decreased ethanol-induced macroscopic, histological and biochemical changes in the gastroduodenal mucosa. 3. Ethanol-induced lesions were detectable as haemorrhagic streaks. Ethanol administration damaged 36% and 25% of the total gastric and duodenal surface, respectively. Melatonin treatment reduced ethanol-induced gastric and duodenal damage to 14% and 8%, respectively. When indomethacin was given together with ethanol, the gastric damaged area was 44% of the total surface, while the duodenal damaged area was 35%; melatonin administration reduced the damage to only 13% of the total gastric surface and to 12% of total duodenal surface. 4. Both stomach and duodenum of ethanol-treated animals showed polymorphonuclear leukocyte (PMN) infiltration. The number of PMN increased more than 600 and 200 times in stomach and duodenum, respectively, following ethanol administration. Melatonin treatment reduced ethanol-induced PMN infiltration by 38% in the stomach and 20% in the duodenum. In indomethacin-ethanol-treated rats, the number of PMN increased by 875% compared to control group in the stomach and by 264% in duodenum. Melatonin administration reduced the indomethacin-ethanol-induced PMN rise by 57% in the stomach and 40% in the duodenum. 5. Gastroduodenal total glutathione (tGSH) concentration and glutathione reductase (GSSG-Rd) activity were significantly reduced following ethanol and indomethacin-ethanol administration. Melatonin ameliorated both the decrease in tGSH concentration as well as the reduction of GSSG-Rd activity elicited by ethanol both in the stomach and duodenum; melatonin was effective against indomethacin-ethanol-induced damage only in the stomach. 6. Ethanol-induced gastroduodenal damage is believed to be mediated by the generation of free radicals. Recently, a number of in vivo and in vitro experiments have shown melatonin to be an effective antioxidant and free radical scavenger; thus, we conclude that the protection by melatonin against ethanol-induced gastroduodenal injury is due, at least in part, to its radical scavenging activity.


Subject(s)
Digestive System/drug effects , Duodenum/drug effects , Ethanol/pharmacology , Melatonin/pharmacology , Stomach/drug effects , Animals , Digestive System/injuries , Male , Melatonin/administration & dosage , Rats , Rats, Sprague-Dawley
9.
Hepatogastroenterology ; 43(10): 898-905, 1996.
Article in English | MEDLINE | ID: mdl-8884311

ABSTRACT

BACKGROUND/AIMS: The protective effect of mela tonin against the damage inflicted by reactive oxygen species during liver ischemia-reperfusion was investigated in male Sprague-Dawley rats using both biochemical and morphological parameters. MATERIALS AND METHODS: For biochemical analyses the levels of lipid peroxidation products [malonaldehyde (MDA) + 4-hydroxyalkenals (4-HDA)], levels of reduced glutathione (GSH) and oxidized glutathione (GSSG), and the activities of GSH peroxidase (GSH-Px), GSH reductase (GSSG-Rd) and glucose-6-phosphatase (G6Pase) were estimated. Also the number of polymorphonuclear neutrophils (PMNs) in injured livers was counted in histological sections. RESULTS: After 40 min of ischemia followed by 60 min of reperfusion the hepatic levels of MDA + 4-HDA increased. Pretreatment of the animals with melatonin abolished the rise in MDA + 4-HDA induced by ischemia-reperfusion. GSH concentrations decreased and GSSG increased during ischemia-reperfusion and, again melatonin counteracted these changes. Additionally, the activities of two antioxidative enzymes (GSH-Px and GSSG-Rd) decreased during the experimental period with melatonin preventing the change in GSSG-Rd. G6Pase activity was not influenced by either ischemia-reperfusion or by melatonin administration. Morphologically, PMN infiltration was obvious in the ischemia-reperfusion damaged liver, a change also partially reversed by melatonin. CONCLUSIONS: In this model of liver ischemia-reperfusion injury, exogenously administered melatonin effectively protected against oxidative damage. The hepatic parameters which illustrated this protection were reduced lipid peroxidation products, lowered PMN infiltration, increased GSH and reduced GSSG levels, and elevated GSSG-Rd activity all of which were observed in melatonin-treated rats in which damage due to ischemia-reperfusion had been induced.


Subject(s)
Free Radical Scavengers/therapeutic use , Liver/blood supply , Melatonin/therapeutic use , Reperfusion Injury/prevention & control , Animals , Free Radical Scavengers/pharmacology , Lipid Peroxidation , Liver/metabolism , Liver/pathology , Male , Melatonin/pharmacology , Oxidative Stress , Rats , Rats, Sprague-Dawley , Reactive Oxygen Species , Reperfusion Injury/metabolism , Reperfusion Injury/pathology , Time Factors
10.
Eur J Pharmacol ; 305(1-3): 239-42, 1996 Jun 03.
Article in English | MEDLINE | ID: mdl-8813560

ABSTRACT

Melatonin protection against in vitro kainic acid-induced oxidative damage in rat brain is shown. Brain disrupted cell homogenates were incubated with different concentrations of kainate and with or without different concentrations of melatonin. The concentration of malonaldehyde and 4-hydroxyalkenals was measured as an index of lipid peroxidation. When administered together with kainate, melatonin produced a concentration-dependent decrease in kainate-induced lipid peroxidation ranging from 20% to 100%. Moreover, when added to the reaction mixture alone, melatonin decreased the basal level of lipid peroxidation compared to controls.


Subject(s)
Brain/metabolism , Excitatory Amino Acid Agonists/pharmacology , Kainic Acid/antagonists & inhibitors , Lipid Peroxidation/drug effects , Melatonin/pharmacology , Neuroprotective Agents/pharmacology , Animals , In Vitro Techniques , Kainic Acid/pharmacology , Male , Malondialdehyde/metabolism , Rats , Rats, Sprague-Dawley
11.
Microsc Res Tech ; 34(2): 144-8, 1996 Jun 01.
Article in English | MEDLINE | ID: mdl-8722709

ABSTRACT

Three experiments employing male and female Syrian hamsters (aged 1, 2, and 8-10 months), male Sprague-Dawley rats (aged 1, 2, and 10 months) and male C57B1 mice (aged 2, 7, 13, and 29 months) examined the effects of age and sex on Mg(2+)-dependent and Ca2+, Mg(2+)-dependent ATPase activity in the Harderian gland. Significant differences due to age and sex were observed in the hamsters and rats but not with age in mice. Generally, male hamsters had significantly higher Mg(2+)-dependent and Ca2+, Mg(2+)-dependent (exception at one timepoint) ATPase activity than did females. Age-matched male and female rats had similar values of Mg(2+)-dependent ATPase activity, but males had significantly higher Ca2+, Mg(2+)-dependent ATPase activity than females at 2 months of age.


Subject(s)
Aging , Ca(2+) Mg(2+)-ATPase/metabolism , Harderian Gland/enzymology , Animals , Cricetinae , Female , Male , Mesocricetus , Mice , Mice, Inbred C57BL , Rats , Rats, Sprague-Dawley , Sex Factors
12.
Biochem Pharmacol ; 51(8): 1095-9, 1996 Apr 26.
Article in English | MEDLINE | ID: mdl-8866832

ABSTRACT

The ability of melatonin to protect against paraquat-induced oxidative damage in rat lung, liver, and serum was examined. Changes in the levels of malondialdehyde (MDA) plus 4-hydroxyalkenals (4-HDA) and reduced and oxidized glutathione concentrations were measured. Paraquat (50 mg/kg) was injected i.p. into either Sprague-Dawley or Wistar rats with or without the co-administration of 5 mg/kg melatonin. Paraquat alone increased MDA + 4-HDA levels in serum and lungs of both rat strains, with these increases being abolished by melatonin co-treatment. Paraquat also decreased reduced glutathione levels and increased oxidized glutathione concentrations in lung and liver; these changes were negated by melatonin. The effect of melatonin on paraquat-induced mortality was also studied. Paraquat at a dose of 79 mg/kg was lethal for 50% of animals within 24 hr; when administered together with melatonin, the LD50 for paraquat increased to 251 mg/kg.


Subject(s)
Herbicides/toxicity , Oxidative Stress , Paraquat/toxicity , Animals , Antioxidants/pharmacology , Glutathione/analogs & derivatives , Glutathione/analysis , Glutathione Disulfide , Lethal Dose 50 , Lipid Peroxidation/drug effects , Liver/metabolism , Lung/metabolism , Malondialdehyde/analysis , Melatonin/pharmacology , Paraquat/antagonists & inhibitors , Rats , Rats, Sprague-Dawley , Rats, Wistar
13.
Mol Cell Endocrinol ; 117(2): 183-8, 1996 Mar 25.
Article in English | MEDLINE | ID: mdl-8737378

ABSTRACT

The ability of melatonin to influence lipopolysaccharide (LPS)-induced genotoxicity was tested using micronuclei as an index in both bone marrow and peripheral blood cells of rats. LPS was given as a single dose of 10 mg/kg. Melatonin (5 mg/kg) was injected prior to LPS administration and thereafter at 6 h intervals to the conclusion of the study (72 h). The number of micronucleated polychromatic erythrocytes increased significantly after LPS administration both in cells from peripheral blood and bone marrow. Melatonin administration to LPS-treated rats highly significantly reduced micronuclei formation in both peripheral blood and bone marrow cells beginning at 24 h after LPS administration and continuing to the end of the study. In blood the increase in micronuclei formation was time-dependent in LPS-treated rats with peak values being reached at 36-48 h. The ability of melatonin to reduce LPS-related genotoxicity is likely related to its antioxidant activity.


Subject(s)
Antimutagenic Agents/pharmacology , DNA Damage , Lipopolysaccharides/toxicity , Melatonin/pharmacology , Mutagens/toxicity , Animals , Bone Marrow/drug effects , Bone Marrow Cells , Erythrocytes/cytology , Erythrocytes/drug effects , Leukocytes, Mononuclear/cytology , Leukocytes, Mononuclear/drug effects , Lipopolysaccharides/antagonists & inhibitors , Male , Micronucleus Tests , Mitomycins/toxicity , Pineal Gland/metabolism , Rats , Rats, Sprague-Dawley , Time Factors
14.
Free Radic Biol Med ; 19(6): 903-9, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8582667

ABSTRACT

The protective effect of melatonin against lipopolysaccharide (LPS)-induced oxidative damage was examined in vitro. Lung, liver, and brain malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) concentrations were measured as indices of induced membrane peroxidative damage. Homogenates of brain, lung, and liver were incubated with LPS at concentrations of either 1, 10, 50, 200, or 400 micrograms/ml for 1 h and, in another study, LPS at a concentration of 400 micrograms/ml for either 0, 15, 30, or 60 min. Melatonin at increasing concentrations from 0.01-3 mM either alone or together with LPS (400 micrograms/ml) was used. Liver, brain, and lung MDA + 4-HDA levels increased after LPS at concentrations of 10, 50, 200 or 400 micrograms/ml; this effect was concentration-dependent. The highest levels of lipid peroxidation products were observed after tissues were incubated with an LPS concentration of 400 micrograms/ml for 60 min; in liver and lung this effect was totally suppressed by melatonin and partially suppressed in brain in a concentration-dependent manner. In addition, melatonin alone was effective in brain at concentrations of 0.1 to 3 mM, in lung at 2 to 3 mM, and in liver at 0.1 to 3 mM; in all cases, the inhibitory effects of melatonin on lipid peroxidation were always directly correlated with the concentration of melatonin in the medium. The results show that the direct effect of LPS on the lipid peroxidation following endotoxin exposure is markedly reduced by melatonin.


Subject(s)
Lipid Peroxidation/drug effects , Lipopolysaccharides/pharmacology , Melatonin/pharmacology , Aldehydes/metabolism , Animals , Brain/metabolism , Free Radicals , Kinetics , Lipopolysaccharides/administration & dosage , Liver/metabolism , Lung/metabolism , Male , Malondialdehyde/metabolism , Rats , Rats, Wistar
15.
Eur J Pharmacol ; 293(4): 327-34, 1995 Dec 07.
Article in English | MEDLINE | ID: mdl-8748685

ABSTRACT

Oxidative damage to the liver of lipopolysaccharide-treated rats was evaluated using four parameters: level of lipid peroxidation, changes in total GSH and GSSG concentrations and hepatic morphology. Bacterial lipopolysaccharide (10 mg/kg b.w.) was injected i.p. either at 6, 16 or 24 h before animals were killed. Lipopolysaccharide increased lipid peroxidation most dramatically when it is injected 6 h before killing. Hepatic total GSH increased after lipopolysaccharide in a time-dependent manner. The highest level of GSSG and largest GSSG/total GSH ratio were also observed in the group of animals injected with lipopolysaccharide 6 h before tissue collection. In a second study, lipopolysaccharide was injected 6 h before the animals were killed, with or without 1 mg/kg b.w. melatonin. Melatonin totally abolished lipopolysaccharide-induced increase in lipid peroxidation, exaggerated the rise in total GSH and reversed the increase in GSSG concentration. The liver showed obvious histological degenerative changes after lipopolysaccharide, effects that were counteracted by melatonin administration. The protection conferred by melatonin is presumably due to its antioxidant activity.


Subject(s)
Antioxidants/pharmacology , Chemical and Drug Induced Liver Injury , Escherichia coli , Lipid Peroxidation/drug effects , Lipopolysaccharides/antagonists & inhibitors , Melatonin/pharmacology , Animals , Glutathione/metabolism , Lipopolysaccharides/toxicity , Liver/metabolism , Liver/pathology , Liver Diseases/metabolism , Liver Diseases/pathology , Male , Rats , Rats, Sprague-Dawley
16.
Biol Signals ; 4(6): 325-30, 1995.
Article in English | MEDLINE | ID: mdl-8688911

ABSTRACT

The hormone synthesized by the pineal gland, melatonin, has been shown to be a direct free radical scavenger both in vivo and in vitro. Thus, it potently protects cells from the damage induced by oxidative agents. In this study, we demonstrate that melatonin increases glutathione peroxidase activity in several tissues from chicks. This stimulation is time dependent and maximal increases are seen 90 min after melatonin injection (500 micrograms/kg intraperitoneally), although enzymatic activity is still elevated 135 min after its administration. No significant increases were detected 45 min after the injection. Glutathione peroxidase is generally considered to be an important antioxidative enzyme because it metabolizes hydrogen peroxide and other hydroperoxides. Thus, melatonin not only is a direct scavenger of toxic radicals but in an avian species, as in mammals, it stimulates the antioxidative enzyme glutathione peroxidase. The ability of melatonin to increase glutathione peroxidase activity is consistent with its general role as an antioxidant.


Subject(s)
Glutathione Peroxidase/metabolism , Melatonin/pharmacology , Oxidative Stress/drug effects , Animals , Brain/enzymology , Chickens/metabolism , Enzyme Induction/drug effects , Erythrocytes/enzymology , Free Radical Scavengers/pharmacology , Harderian Gland/enzymology , Nerve Tissue Proteins/metabolism , Organ Specificity , Viscera/enzymology
17.
FASEB J ; 9(12): 1205-10, 1995 Sep.
Article in English | MEDLINE | ID: mdl-7672513

ABSTRACT

Melatonin protection against in vitro kainic acid-induced oxidative damage in homogenates from different rat brain regions is shown. Brain-disrupted cell homogenates from cerebral cortex, cerebellum, hippocampus, hypothalamus, and corpus striatum were incubated with kainate (11.7 mM) with or without different concentrations of melatonin (0.1-4 mM). The concentration of malonaldehyde and 4-hydroxyalkenals was measured as an index of lipid peroxidation. Wistar and Sprague-Dawley rats were used. When administered with kainate, melatonin markedly reduced lipid peroxidation in every brain region of both rat strains. The reduction in lipid peroxidation by melatonin was concentration-dependent and varied from 10% to 100%. The protection conferred by melatonin is likely due, at least in part, to its newly discovered, free radical scavenging ability.


Subject(s)
Brain/metabolism , Kainic Acid/pharmacology , Lipid Peroxidation/drug effects , Melatonin/pharmacology , Animals , Cerebellum/metabolism , Cerebral Cortex/metabolism , Hippocampus/metabolism , Kainic Acid/antagonists & inhibitors , Male , Rats , Rats, Sprague-Dawley , Rats, Wistar
18.
J Cell Biochem ; 58(4): 436-44, 1995 Aug.
Article in English | MEDLINE | ID: mdl-7593265

ABSTRACT

The protective effect of melatonin on lipopolysaccharide (LPS)-induced oxidative damage in phenobarbital-treated rats was measured using the following parameters: changes in total glutathione (tGSH) concentration, levels of oxidized glutathione (GSSG), the activity of the antioxidant enzyme glutathione peroxidase (GSH-PX) in both brain and liver, and the content of cytochrome P450 reductase in liver. Melatonin was injected intraperitoneally (ip, 4mg/kg BW) every hour for 4 h after LPS administration; control animals received 4 injections of diluent. LPS was given (ip, 4 mg/kg) 6 h before the animals were killed. Prior to the LPS injection, animals were pretreated with phenobarbital (PB), a stimulator of cytochrome P450 reductase, at a dose 80 mg/kg BW ip for 3 consecutive days. One group of animals received LPS together with Nw-nitro-L-arginine methyl ester (L-NAME), a blocker of nitric oxide synthase (NOS) (for 4 days given in drinking water at a concentration of 50 mM). In liver, PB, in all groups, increased significantly both the concentration of tGSH and the activity of GSH-PX. When the animals were injected with LPS the levels of tGSH and GSSG were significantly higher compared with other groups while melatonin and L-NAME significantly enhanced tGSH when compared with that in the LPS-treated rats. Melatonin alone reduced GSSG levels and enhanced the activity of GSH-PX in LPS-treated animals. Additionally, LPS diminished the content of cytochrome P450 reductase with this effect being largely prevented by L-NAME administration. Melatonin did not change the content of P450 either in PB- or LPS-treated animals.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Brain/metabolism , Glutathione Peroxidase/metabolism , Glutathione/metabolism , Lipopolysaccharides/toxicity , Liver/metabolism , Melatonin/pharmacology , Phenobarbital/pharmacology , Analysis of Variance , Animals , Arginine/analogs & derivatives , Arginine/pharmacology , Brain/drug effects , Brain/pathology , Escherichia coli , Glutathione/analogs & derivatives , Glutathione Disulfide , Lipopolysaccharides/antagonists & inhibitors , Liver/drug effects , Liver/pathology , Male , NADPH-Ferrihemoprotein Reductase/metabolism , NG-Nitroarginine Methyl Ester , Nitric Oxide/antagonists & inhibitors , Nitric Oxide Synthase/antagonists & inhibitors , Oxidation-Reduction , Rats , Rats, Sprague-Dawley
19.
J Pineal Res ; 19(1): 1-6, 1995 Aug.
Article in English | MEDLINE | ID: mdl-8609590

ABSTRACT

Carbon tetrachloride (CCl4) exerts its toxic effects by the generation of free radicals. In this study we investigated whether melatonin, a potent free radical scavenger, could prevent the deleterious effects of CCl4. Liver homogenates and liver microsomes were incubated with CCl4 in the presence of melatonin and lipid peroxidation and glucose-6 phosphatase (G6Pase) activity were determined. All doses of CCl4 (1, 0.5, 0.1 mM) produced significantly high levels of lipid peroxidation, as reflected by increased levels of malonaldehyde and 4-hydroxyalkenals, in both liver homogenates and liver microsomes. These doses of CCl4 concommitantly reduced the activity of microsomal G6Pase. Co-incubation with melatonin dose-dependently (2, 1, 0.5 mM) inhibited the production of lipid peroxidation, but it was unable to restore the activity of G6Pase. In in vivo studies, rats were also treated with melatonin (10 mg/kg, i.p.), given 30 min before and 60 min after the administration of CCl4 (5 ml/kg, i.p.). Significantly elevated levels of lipid peroxidation were measured in the liver and kidney. Melatonin prevented the CCl4-induced lipid peroxidation in the kidney, but not in the liver. These data suggest that melatonin may provide protection against some of the damaging effects of CCl4, possibly due to its ability to scavenge toxic free radicals.


Subject(s)
Carbon Tetrachloride/antagonists & inhibitors , Carbon Tetrachloride/toxicity , Glucose-6-Phosphatase/antagonists & inhibitors , Lipid Peroxidation/drug effects , Melatonin/pharmacology , Animals , Free Radical Scavengers/pharmacology , In Vitro Techniques , Liver/drug effects , Liver/metabolism , Male , Malondialdehyde/metabolism , Microsomes, Liver/drug effects , Microsomes, Liver/metabolism , Rats , Rats, Sprague-Dawley
20.
J Pineal Res ; 19(1): 51-6, 1995 Aug.
Article in English | MEDLINE | ID: mdl-8609595

ABSTRACT

The ability of melatonin to modify H2O2-induced lipid peroxidation in brain homogenates was determined. The concentrations of brain malonaldehyde (MDA) and 4-hydroxyalkenals (4-HDA) were assayed as an index of induced membrane oxidative damage. Homogenates from five different regions of the brain (cerebral cortex, cerebellum, hippocampus, hypothalamus, and corpus striatum) derived from two different strains of rats, Sprague-Dawley and Wistar, were incubated with either H2O2 (5 mM) alone or H2O2 together with melatonin at increasing concentrations ranging from 0.1 to 4 mM. The basal level of lipid peroxidation was strain-dependent and about 100% higher in homogenates from the brain of Wistar rats than those measured in Sprague-Dawley rats. MDA + 4-HDA levels increased after H2O2 treatment in homogenates obtained from each region of the brain in both rat strains but the sensitivity of the homogenates from Sprague-Dawley rats was greater than that for the homogenates from Wistar rats (increases after H2O2 from 45 to 165% compared 20 to 40% for Sprague-Dawley and Wistar rats, respectively). Melatonin co-treatment reduced H2O2-induced lipid peroxidation in brain homogenates in a concentration-dependent manner; the degree of protection against lipid peroxidation was similar in all brain regions.


Subject(s)
Brain/drug effects , Brain/metabolism , Hydrogen Peroxide/antagonists & inhibitors , Hydrogen Peroxide/toxicity , Lipid Peroxidation/drug effects , Melatonin/pharmacology , Animals , Cerebellum/drug effects , Cerebellum/metabolism , Cerebral Cortex/drug effects , Cerebral Cortex/metabolism , Corpus Striatum/drug effects , Corpus Striatum/metabolism , Hippocampus/drug effects , Hippocampus/metabolism , Hypothalamus/drug effects , Hypothalamus/metabolism , In Vitro Techniques , Male , Malondialdehyde/metabolism , Rats , Rats, Sprague-Dawley , Rats, Wistar , Tissue Distribution
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