ABSTRACT
Eimeria truncata oocysts were isolated from a naturally infected goose stock. Domestic geese and ducks were inoculated with sporulated oocysts. Meronts, merozoites, macro- and microgamonts, and microgametes were studied with the light and electron microscope. These stages were located in the epithelial cells of the kidney tubules only. The meronts and merozoites showed the characteristic fine structures of Eimeria species. The macrogamonts only possessed one type of wall-forming bodies. It resembled the wall-forming bodies type I of other Eimeria species. One of the most prominent observations seemed to be the occurrence of osmiophilic granular material in the parasitophorous vacuoles of all endogenous stages of E. truncata.
Subject(s)
Coccidiosis/veterinary , Eimeria/ultrastructure , Geese/parasitology , Poultry Diseases/parasitology , Animals , Cell Nucleus/ultrastructure , Coccidiosis/parasitology , Cytoplasm/ultrastructure , Eimeria/growth & development , Kidney Tubules/parasitology , Microscopy, Electron , Mitochondria/ultrastructure , Organoids/ultrastructureABSTRACT
Purified protease was obtained from Pseudomonas aeruginosa. It stimulated in rabbits the production of antibodies, which could be demonstrated mainly in an indirect hemagglutination test. Injected intracutaneously into rabbits, the protease caused an increased permeability in the capillaries and in higher doses bleedings. Immunization with the protease reduced the development of these lesions. Similar findings were obtained in experiments with mice. An antiserum prepared in rabbits against purified protease did not protect mice against infection with P. aeruginosa. However, a small protective effect could be observed after active immunization.