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1.
Article in English | MEDLINE | ID: mdl-38401093

ABSTRACT

Objective: Analyzing the impact of nursing workforce development, training and standardization on hybrid operating theatres. Methods: Thirty nurses in the mixed operating room of the First Affiliated Hospital of Nanchang University from January 2021 to December 2021 were selected as the control group to receive routine nursing management and training methods and another thirty nurses were selected as the experimental group to receive nursing team construction, training and standardized management based on conventional methods. Nurses' theoretical and operational scores, nurses' satisfaction, surgeon satisfaction with nurses, and nursing service quality scores were compared between two groups at baseline and after intervention. Results: After the intervention, nurses in both groups had a significant improvement in theoretical and operational scores than those at baseline, and nurses in the experimental group had better scores than those in the control group, The difference was statistically significant (P = .002, P = .004). Nursing quality of surgical preparation, environmental management, surgical safety, and instrument management in the intervention group were significantly better than those at baseline and better than those in the control group. The difference was statistically significant (P = .001, P = .001, P = .001, P = .001). Satisfaction of nurses and doctors in the intervention group was significantly better than those at baseline and better than those in the control group. The difference was statistically significant (P = .002, P = .001). Conclusion: The effect of nursing team construction and training and standardized management of hybrid operating Room was ideal, which can improve nurses' theoretical knowledge and practical skills, and enhance the satisfaction of nurses and surgeons, providing patients with higher quality nursing services, which is worth adopting.

2.
Life Sci ; 329: 121926, 2023 Sep 15.
Article in English | MEDLINE | ID: mdl-37437652

ABSTRACT

Cardiac fibrosis, a common pathology in inherited and acquired heart diseases, necessitates the identification of diagnostic and therapeutic targets. Methyltransferase Like 1 (METTL1), an enzyme responsible for RNA modification by methylating guanosine to form m7G, is an emerging area of research in understanding cellular processes and disease pathogenesis. Dysregulation of m7G modification has been implicated in various diseases. However, the role of METTL1 in cardiac fibrosis remains unclear. This study aimed to investigate the role of METTL1 in myocardial infarction-induced heart failure and cardiac fibrosis. Our findings demonstrate that elevated METTL1-mediated RNA m7G methylation is observed in cardiac fibrosis tissues and TGF-ß1-induced cardiac fibroblast proliferation and myofibroblast transformation. Furthermore, fibroblast-specific knockout of METTL1 attenuated myocardial infarction-induced heart failure and cardiac fibrosis. Additionally, METTL1 knockout decreased m7G methylated fibrotic genes and impaired their translation efficiency. These results suggest a novel pro-fibrosis role of METTL1-mediated RNA m7G methylation, highlighting its potential as a therapeutic target in cardiac fibrosis.


Subject(s)
Heart Failure , Myocardial Infarction , Humans , Myocardial Infarction/complications , Myocardial Infarction/genetics , Myocardial Infarction/pathology , Fibroblasts/pathology , Heart Failure/genetics , Heart Failure/pathology , Fibrosis , RNA , Methyltransferases/genetics
3.
Biochem Biophys Res Commun ; 504(1): 61-67, 2018 09 26.
Article in English | MEDLINE | ID: mdl-30190124

ABSTRACT

Intracerebral haemorrhage (ICH) is a common and devastating cerebrovascular disease with high morbidity and mortality, and its pathophysiological mechanisms were complex and still unclear. Increasing researches reported that microRNAs (miRNAs) played an important role in ICH-induced brain injury and microglial activation. In this study, we investigated the biological function of miR-590-5p and explored its molecular mechanism in ICH mice. The results of qRT-PCR showed that miR-590-5p expression level was down-regulated in perihematomal brain samples of ICH mice compared with that of sham group. In LPS-induced microglia cells, miR-590-5p level was also down-regulated at 24 h post-LPS compared with that of control group. Moreover, miR-590-5p overexpression remarkably increased the cerebral water content and neurological severity scores compared with that of scramble group in ICH mice. The production of inflammatory cytokine including IL-6, interleukin (IL)-1ß, and tumor necrosis factor (TNF)-α in ICH mice was notably inhibited by miR-590-5p overexpression. Furthermore, the results of dual-luciferase reporter assay indicated that Pellino-1 (Peli1) was a direct target of miR-590-5p. MiR-590-5p overexpression remarkably inhibited the Peli1 gene expression both mRNA and protein levels. In addition, Peli1 overexpression partly abrogated the inhibitory effect of miR-590-5p mimic. Taken together, these datas suggested that miR-590-5p attenuated brain injury in ICH mice through inhibiting Peli1 gene expression, indicating that miR-590-5p may be a promising molecular target for ICH treatment.


Subject(s)
Brain Injuries/genetics , Cerebral Hemorrhage/complications , Gene Expression Regulation , MicroRNAs/metabolism , Nuclear Proteins/genetics , Ubiquitin-Protein Ligases/genetics , Animals , Brain Injuries/etiology , Brain Injuries/metabolism , Cells, Cultured , Cerebral Hemorrhage/genetics , Cerebral Hemorrhage/metabolism , Down-Regulation , Male , Mice, Inbred C57BL , Microglia/metabolism , Nuclear Proteins/metabolism , Ubiquitin-Protein Ligases/metabolism
4.
Inflammation ; 36(5): 1101-6, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23640201

ABSTRACT

Inflammation plays an important role in the occurrence and development of fibrosis. Lipoxins (LXs) and BML-111 (lipoxin A4 agonist) have been approved for potent anti-inflammatory properties. Previously, we and others had showed LXs and BML-111 could protect acute hepatic injury, inhibit the growth and invasion of hepatic tumor. However, there are few reports dealing with their effects on hepatic fibrosis. To explore whether LXs and the analog could interrupt the process of hepatic fibrosis, the effects of BML-111 on tetrachloride-induced hepatic fibrosis were observed and the possible mechanism were discussed. Sprague-Dawley rats were induced liver fibrosis by carbon tetrachloride (CCl4) for 10 weeks with or without BML-111, and the histopathology and collagen content were employed to quantify hepatic necro-inflammation and fibrosis. Moreover, the expression levels of α-smooth muscle actin (α-SMA), transforming growth factor-ß1 (TGF-ß1), and platelet-derived growth factor (PDGF) were examined via Western blot or ELISA. Rats treated with BML-111 improved hepatic necro-inflammation and inhibited hepatic fibrosis in association with reduction of α-SMA expression and decreased collagen deposition. Furthermore, BML-111 could downregulate the expressions of TGF-ß1 and PDGF significantly. BML-111 played a critical protective role in CCl4-induced hepatic fibrosis through inhibiting the levels of TGF-ß1 and PDGF in rats.


Subject(s)
Heptanoic Acids/pharmacology , Liver Cirrhosis/prevention & control , Platelet-Derived Growth Factor/metabolism , Receptors, Lipoxin/agonists , Transforming Growth Factor beta1/metabolism , Actins/biosynthesis , Animals , Carbon Tetrachloride , Collagen/biosynthesis , Down-Regulation/drug effects , Gene Expression , Inflammation/drug therapy , Liver/drug effects , Liver/metabolism , Liver/pathology , Liver Cirrhosis/chemically induced , Platelet-Derived Growth Factor/biosynthesis , Rats , Rats, Sprague-Dawley , Transforming Growth Factor beta1/biosynthesis , Transforming Growth Factor beta1/blood
6.
Nan Fang Yi Ke Da Xue Xue Bao ; 30(8): 1950-2, 2010 Aug.
Article in Chinese | MEDLINE | ID: mdl-20813712

ABSTRACT

OBJECTIVE: To observe the changes in the expression of glucose transporter-3 (GLUT3) in the cerebral cortex of rats during aging and investigate the role of GLUT3 in the aging process of the nervous system. METHODS: The cerebral tissues were collected from rats of 3, 18, 24, and 30 months old (10 in each age group), and the expression of GLUT3 in the cerebral cortex was detected by immunohistochemistry. RESULTS: Under optical microscope, GLUT3-positive cells were found in every group. Within the age range of 3 to 8 months, GLUT3-positive cells increased significantly with age (P<0.01), but at 24-30 months of age, the number of GLUT3-positive cells reduced significant with age (P<0.01). CONCLUSION: The expression changes of GLUT3 ir the cerebral cortex of rats during aging indicate that GLUT3 plays an important role in the maturation and aging of the nervous system.


Subject(s)
Aging , Cerebral Cortex/metabolism , Glucose Transporter Type 3/metabolism , Animals , Brain/metabolism , Male , Rats , Rats, Sprague-Dawley
7.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 39(4): 404-8, 2010 07.
Article in Chinese | MEDLINE | ID: mdl-20731041

ABSTRACT

OBJECTIVE: To investigate the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in hippocampus of rats with aging. METHODS: Paraffin sections of brain tissue of rats at the age of 3, 18, 24, 30 months were stained by immunohistochemistry, the expression of VEGF and MVD was quantitatively analyzed. RESULTS: Innunohistochemical staining showed that the VEGF-positive cells were mainly pyramidal neuron in hippocampus; the intensity of VEGF-positivity in neuron cells was decreased with the aging (P<0.05). The MVD in hippocampus was also decreased with the aging of rats (P<0.05). CONCLUSION: Increasing VEGF contents and improving blood circulation in brain tissue may prevent or treat vascular dementia and cerebrovascular diseases.


Subject(s)
Aging , Hippocampus/blood supply , Hippocampus/metabolism , Vascular Endothelial Growth Factor A/metabolism , Animals , Capillaries/pathology , Male , Rats , Rats, Sprague-Dawley , Vascular Endothelial Growth Factor A/genetics
8.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 39(1): 43-8, 2010 01.
Article in Chinese | MEDLINE | ID: mdl-20175235

ABSTRACT

OBJECTIVE: To examine the distribution of glucose transport 3 (GLUT 3) in different brain regions of aged rats and to investigate its role in ageing process of the nervous system. METHODS: The GLUT 3 expression in different brain regions was examined with immunohistochemical method in rats aged 3, 18 and 30 months, respectively. RESULTS: The number of GLUT 3-positive cells varied in the different brain regions in rats of all age groups (P<0.01); the CA1 region contained the greatest number of positive cells,and fewer in the motor cortex and cerebellum. The number of GLUT 3-positive cells was reduced in the brain of aged rats (P<0.01); and the neural cells in 4 different brain regions presented with large cell body and loose alignment. CONCLUSION: The expression of GLUT 3 decreased in aged rats, which suggests that GLUT 3 may be involved in the ageing process of nervous system.


Subject(s)
Aging/metabolism , Brain/metabolism , Glucose Transporter Type 3/metabolism , Hippocampus/metabolism , Animals , Male , Rats , Rats, Sprague-Dawley
9.
Health Educ Behav ; 36(2): 259-77, 2009 Apr.
Article in English | MEDLINE | ID: mdl-17620666

ABSTRACT

The purpose of this study is to see whether witnessing a very violent act at any point in the life course is associated with depressive symptoms and alcohol use in late life. The data come from a nationwide probability sample of older adults (N = 1,498). The findings reveal that witnessing violence is associated with more symptoms of depression for older women but not older men. In contrast, seeing a violent act is associated with greater alcohol consumption for older men but not older women. The results further indicate that age at first exposure to a violent act is not consistently associated with current depression or alcohol intake, suggesting that people who see something violent happen at any time in life may be at risk. The implications of these findings for designing interventions to help those who witness violent acts are discussed.


Subject(s)
Alcoholism/epidemiology , Depression/epidemiology , Violence , Age Factors , Aged , Female , Humans , Life Change Events , Male , Sex Factors , Socioeconomic Factors
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