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1.
Med Hypotheses ; 63(4): 630-2, 2004.
Article in English | MEDLINE | ID: mdl-15325007

ABSTRACT

Obstructive sleep apnea (OSA) is a disease with significant morbidity, increased risk of accidents attributed to daytime somnolence, and has been associated with cardiovascular complications. The treatment of choice for OSA is nasal continuous positive airway pressure (nCPAP). Some OSA patients, however, are unable to benefit from this therapy as they find nCPAP intolerable due to the related nasal inflammation. It is hypothesized that nCPAP may cause nasal inflammation in these patients by inducing changes in the expression of genes that encode interleukins (IL-3, IL-4, IL-6, IL-8, IL-13) or adhesion molecules (i.e., ICAM-1) in T-helper lymphocytes. An understanding of the underlying inflammatory mechanism could lead to specific interventions that render nCPAP therapy tolerable for these individuals.


Subject(s)
Continuous Positive Airway Pressure/adverse effects , Cytokines/immunology , Gene Expression Regulation/immunology , Rhinitis/etiology , Rhinitis/immunology , Sleep Apnea, Obstructive/immunology , Sleep Apnea, Obstructive/therapy , T-Lymphocytes, Helper-Inducer/immunology , Clinical Trials as Topic , Continuous Positive Airway Pressure/methods , Evidence-Based Medicine , Humans , Models, Biological , Sleep Apnea, Obstructive/pathology , Treatment Outcome
2.
Med Hypotheses ; 59(6): 667-9, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12445507

ABSTRACT

Chromosomal instability (CIN) is present in most colorectal cancers, though the mechanism for these genetic aberrations is unclear. An explanation may lie in the possible link between JC virus (JCV) Mad-1 strain, found in colorectal cancers, and aneuploid neoplasia. It is proposed here to test the hypothesis that detection of JCV in colorectal cancer patients may serve as a clinically useful biomarker for the presence of colorectal tumors. This may be tested by looking for any correlation that may exist between JCV DNA, viral proteins, and anti-JCV anti-sera detected in samples of stool, blood, and urine obtained from patients with colorectal neoplasm compared with normal age-matched controls.


Subject(s)
Colorectal Neoplasms/genetics , Colorectal Neoplasms/virology , JC Virus/isolation & purification , Biomarkers , Chromosome Fragility , Colorectal Neoplasms/complications , Humans , Tumor Virus Infections/complications
3.
Med Hypotheses ; 55(1): 1-4, 2000 Jul.
Article in English | MEDLINE | ID: mdl-11021317

ABSTRACT

The generation and accumulation of genetic mutations have been associated with carcinogenesis. Epidemiological and experimental evidence implicate parvoviruses in growth inhibition (oncosuppression) and selective destruction (oncolysis) of tumor cells. It is proposed here that parvoviruses can preferentially target genetically unstable tumor cells, which are deficient in DNA repair mechanisms. This selective strategy may serve as a virus-based therapeutic approach against cancer.


Subject(s)
Neoplasms/prevention & control , Parvoviridae Infections/physiopathology , Parvovirus/genetics , Animals , Humans , Neoplasms/epidemiology , Parvoviridae Infections/epidemiology , Tumor Cells, Cultured
4.
Med Hypotheses ; 55(1): 69-72, 2000 Jul.
Article in English | MEDLINE | ID: mdl-11021331

ABSTRACT

The variety of mutations associated with carcinogenesis, along with variations in penetrance and environmental factors, complicate the genetic screening for cancer predisposition. It is proposed here that the detection of inherent genome instability as determined by increased mutagen susceptibility may enhance the identification of populations at risk for cancer. In support for this hypothesis, our analysis reveals a strong association between mutagen-induced chromosomal instability in peripheral blood lymphocytes and the propensity for cancers of oral cavity, pharynx, larynx, and lung. DNA instability in response to a variety of mutagens identifies patients with gastrointestinal, brain, endocrine, breast, skin, and hematologic tumors as well as individuals with cancer family syndromes. Induced genome instability therefore appears to be strongly linked to cancer predisposition, and prospective studies may yield a screening test utilizing a panel of mutagens to better identify populations at risk.


Subject(s)
Genome, Human , Neoplasms/genetics , Chromosomes, Human/drug effects , Disease Susceptibility , Genetic Predisposition to Disease , Humans , Mass Screening/methods , Models, Genetic , Mutagenicity Tests , Mutagens/pharmacology , Mutation , Neoplasms/diagnosis , Neoplasms/epidemiology
5.
Virus Genes ; 20(3): 209-16, 2000.
Article in English | MEDLINE | ID: mdl-10949947

ABSTRACT

n-Butyrate, an inhibitor of G1-to-S transition inhibits papovavirus DNA replication in cell culture. To explore the efficacy of n-butyrate in vivo and to better understand its mechanism, we studied the effect of n-butyrate on viral DNA replication in mice acutely infected with polyomavirus and in the papovavirus-infected cells in culture. Newborn mice treated with n-butyrate stop growing and become runted. When infected with polyomavirus, these mice show a strong overall inhibition of viral DNA. However, a notable exception to this was the continued viral DNA replication in the differentiated mouse keratinocytes and renal epithelial cells as determined by in situ hybridization. n-Butyrate significantly inhibited viral DNA replication in the cultured IDL cells, and in polyomavirus-infected C2C12 myoblasts based on Southern blot analysis and in situ hybridization. DNA polymerase alpha (but not DNA polymerase beta) and the characteristic nuclear expression of PCNA were both inhibited in the n-butyrate treated IDL and C2C12 cells. n-Butyrate, therefore, inhibited host and viral DNA synthesis in the undifferentiated cells.


Subject(s)
Bovine papillomavirus 1/physiology , Butyrates/pharmacology , DNA Replication , DNA, Viral/biosynthesis , Polyomavirus/physiology , Animals , Animals, Newborn , Blotting, Southern , Bovine papillomavirus 1/drug effects , Bovine papillomavirus 1/metabolism , Cell Nucleus/metabolism , Cells, Cultured , DNA Polymerase I/antagonists & inhibitors , In Situ Hybridization , Mice , Mice, Inbred BALB C , Polyomavirus/drug effects , Polyomavirus/metabolism , Proliferating Cell Nuclear Antigen/metabolism
6.
Cancer Genet Cytogenet ; 118(2): 144-7, 2000 Apr 15.
Article in English | MEDLINE | ID: mdl-10748295

ABSTRACT

Supernumerary ring chromosomes varying with respect to both size and number were found as the primary cytogenetic anomaly in a rare benign soft tissue chondroma resected from the floor of the mouth of a 3-year-old girl. Reverse fluorescence in situ hybridization paint probes prepared by polymerase chain reaction from microdissected rings produced fluorescent signal over two large but discontinuous parts of the chromosome 12 long arm, subdivided into four regions. This case expands the spectrum of mesenchymal neoplasms in which ring chromosomes have been described as the primary genetic anomaly. A review of the literature reporting similar findings in other soft tissue tumors further supports the possibility that low-level amplification of chromosome 12 long-arm regions may contribute to abnormal cellular proliferation in a variety of mesenchymal tumors. Genes implicated in the control of the cell cycle such as sarcoma amplified sequence (SAS), the human homolog of the murine double-minute type 2 gene (MDM-2), proto-oncogenes CHOP/GADD153, GLI, A2MR, cyclin-dependent kinase (CDK4), and the high mobility group (HMGIC) gene implicated in mesenchymal tumorigenesis are all located on the long arm of chromosome 12. Chromosomal abnormalities involving the 12q13-q15 region are associated with a wide range of benign soft tissue tumors and sarcomas.


Subject(s)
Chondroma/genetics , Chromosomes, Human, Pair 12 , Mouth Neoplasms/genetics , Ring Chromosomes , Child, Preschool , Chondroma/pathology , Female , Humans , In Situ Hybridization, Fluorescence , Karyotyping , Mouth Neoplasms/pathology
7.
Med Hypotheses ; 47(1): 1-9, 1996 Jul.
Article in English | MEDLINE | ID: mdl-8819107

ABSTRACT

Genetic instability characterized by the accumulation of mutations of tumor suppressor genes and oncogenes appears to be associated with carcinogenesis in colorectal and other cancers. Mutations of DNA polymerase beta (pol beta) and related chromosomal alterations appear to be consistent with the causal role of a "mutator phenotype' in carcinogenesis. However, homozygous knockout pol beta mutations appear to interfere with embryogenesis. Increased pol beta activity (i.e. relative to pol alpha activity) has been associated with cell cycle arrest. The related aphidicolin-resistant DNA replication has been observed primarily in differentiating cells, including the mammalian blastocyst, adrenal cortex, thyroid, anterior pituitary, and the mechanism of endoreduplication (amitotic over-replication of DNA) can be traced to lower eukaryotes. This increased activity in relation to terminal commitment is inconsistent with a simple "DNA repair' view of pol beta. It is therefore proposed that pol beta may play a more fundamental role in cellular differentiation through involvement in a putative subgenomic DNA replication-based model of terminal gene expression. Thus genetic instability, loss of differentiation, and carcinogenesis may result from aberration(s) or "derailment' of such replication-based mechanism of terminal gene expression. It is suggested to examine the relationship of DNA pol beta to genomic instability and carcinogenesis using genetic analyses and antisense technology with possible applications for gene therapy against colorectal cancer.


Subject(s)
Colorectal Neoplasms/therapy , DNA Polymerase I/genetics , DNA Replication , Genetic Therapy , Neoplasms/therapy , Animals , Blastocyst/physiology , Cell Cycle , Cell Differentiation , Cell Division , Colorectal Neoplasms/genetics , DNA Polymerase I/biosynthesis , Embryonic and Fetal Development , Genes, Tumor Suppressor , Humans , Mammals , Models, Biological , Mutation , Neoplasms/genetics , Oncogenes
8.
Virus Genes ; 11(2-3): 239-57, 1995.
Article in English | MEDLINE | ID: mdl-8828150

ABSTRACT

Historically, viral evolution has often been considered from the perspective of the ability of the virus to maintain viral pathogenic fitness by causing disease. A predator-prey model has been successfully applied to explain genetically variable quasi-species of viruses, such as influenza virus and human immunodeficiency virus (HIV), which evolve much faster rates than the host. In contrast, small DNA viruses (polyomaviruses, papillomaviruses, and parvoviruses) are species specific but are stable genetically, and appear to have co-evolved with their host species. Genetic stability is attributable primarily to the ability to establish and maintain a benign persistent state in vivo and not to the host DNA proofreading mechanisms. The persistent state often involves a cell cycle-regulated episomal state and a tight linkage of DNA amplification mechanisms to cellular differentiation. This linkage requires conserved features among viral regulatory proteins, with characteristic host-interactive domains needed to recruit and utilize host machinery, thus imposing mechanistic constrains on possible evolutionary options. Sequence similarities within these domains are seen amongst all small mammalian DNA viruses and most of the parvo-like viruses, including those that span the entire spectrum of evolution of organisms from E. coli to humans that replicate via a rolling circle-like mechanism among the entire spectrum of organisms throughout evolution from E. coli to humans. To achieve benign inapparent viral persistence, small DNA viruses are proposed to circumvent the host acute phase reaction (characterized by minimal inflammation) by mechanisms that are evolutionarily adapted to the immune system and the related cytokine communication networks. A striking example of this is the relationship of hymenoptera to polydnaviruses, in which the crucial to the recognition of self, development, and maintenance of genetic identity of both the host and virus. These observations in aggregate suggest that viral replicons are not recent "escapies" of host replication, but rather provide relentless pressure in driving the evolution of the host through cospeciation.


Subject(s)
DNA Viruses/genetics , DNA Viruses/immunology , Evolution, Molecular , Adaptation, Physiological/immunology , Animals , Cytokines/immunology , Eukaryotic Cells/immunology , Humans , Immune System/immunology , Immunity , Phylogeny , Selection, Genetic , Vertebrates/immunology , Vertebrates/virology
9.
J Virol ; 68(8): 4785-96, 1994 Aug.
Article in English | MEDLINE | ID: mdl-8035479

ABSTRACT

Small DNA viruses are dependent on the interaction of early proteins (such as large T antigen) with host p53 and Rb to bring about the G1-to-S cell cycle transition. The large DNA viruses are less dependent on host regulatory genes since additional early viral proteins (such as viral DNA polymerase, DNA metabolic enzymes, and other replication proteins) are involved in DNA synthesis. A highly conserved domain of large T antigen (similar to the p53-binding region) exclusively identifies papovavirus, parvovirus, and papillomaviruses from all other larger DNA viruses and implies a conserved interaction with host regulatory genes. In this report, we show that 3 to 6 mM butyrate, a general cell cycle blocker implicated in inhibition of the G1-to-S transition, inhibits DNA replication of polyomavirus and human papillomavirus type 11 but not the replication of larger DNA viruses such as adenovirus types 2 and 5, herpes simplex virus type 1, Epstein-Barr virus, and cytomegalovirus, which all bypass the butyrate-mediated cell cycle block. This butyrate effect on polyomavirus replication is not cell type specific, nor does it depend on the p53 or Rb gene, as inhibition was seen in fibroblasts with intact or homozygous deleted p53 or Rb, 3T6 cells, keratinocytes, C2C12 myoblasts, and 3T3-L1 adipocytes. In addition, butyrate did not inhibit expression of polyomavirus T antigen. The antiviral effect of butyrate involves a form of imprinted state, since pretreatment of cells with 3 mM butyrate inhibits human papillomavirus type 11 DNA replication for at least 96 h after its removal. Butyrate, therefore, serves as a molecular tool in dissecting the life cycle of smaller DNA viruses from that of the larger DNA viruses in relation to the cell cycle.


Subject(s)
Adenoviridae/physiology , Butyrates/pharmacology , Herpesviridae/physiology , Papillomaviridae/physiology , Polyomavirus/physiology , Virus Replication/drug effects , Adenoviridae/drug effects , Animals , Butyric Acid , Cell Cycle/drug effects , Cell Line , HeLa Cells , Herpesviridae/drug effects , Humans , Mice , Papillomaviridae/drug effects , Polyomavirus/drug effects
10.
J Acquir Immune Defic Syndr (1988) ; 6(8): 913-8, 1993 Aug.
Article in English | MEDLINE | ID: mdl-8391077

ABSTRACT

We used the polymerase chain reaction, in situ hybridization, and immunohistochemical stains against latent membrane protein, CD23, and Epstein-Barr viral nuclear antigens 1 and 2 to identify Epstein-Barr virus (EBV) in fixed and unfixed (cryopreserved) AIDS-related lymphoma (ARL) specimens. The study included 17 cases of large-cell (immunoblastic) lymphoma, 11 cases of small non-cleaved cell lymphoma, and two cases of Hodgkin's disease. The EBV DNA was more frequently detected by polymerase chain reaction in cryopreserved specimens (94%) than in fixed specimens (17%). Significantly, the immunohistochemical and in situ hybridization studies detected evidence of EBV in only a small (< 10%) subset of the cells in 27 of 30 ARL specimens. We conclude that tissue fixation reduced the ability to detect EBV in ARL by polymerase chain reaction and that EBV was detectable in only a minority of cells in most ARL tissues.


Subject(s)
Herpesvirus 4, Human/isolation & purification , Hodgkin Disease/microbiology , Lymphoma, AIDS-Related/microbiology , Lymphoma, Large-Cell, Immunoblastic/microbiology , Lymphoma, Non-Hodgkin/microbiology , Antigens, Viral/analysis , Cryopreservation , DNA, Viral/analysis , DNA-Binding Proteins/analysis , Epstein-Barr Virus Nuclear Antigens , Fluorescent Antibody Technique , Formaldehyde , Herpesvirus 4, Human/genetics , Herpesvirus 4, Human/immunology , Hodgkin Disease/etiology , Humans , Immunoblotting , Immunohistochemistry , In Situ Hybridization , Lymphoma, Large-Cell, Immunoblastic/etiology , Lymphoma, Non-Hodgkin/etiology , Polymerase Chain Reaction , Receptors, IgE/analysis , Tissue Fixation
11.
Proc Natl Acad Sci U S A ; 90(9): 4117-21, 1993 May 01.
Article in English | MEDLINE | ID: mdl-8483926

ABSTRACT

Although most RNA viral genomes (and related cellular retroposons) can evolve at rates a millionfold greater than that of their host genomes, some of the small DNA viruses (polyomaviruses and papillomaviruses) appear to evolve at much slower rates. These DNA viruses generally cause host species-specific inapparent primary infections followed by life-long, benign persistent infections. Using global progressive sequence alignments for kidney-specific Polyomaviridae (mouse, hamster, primate, human), we have constructed parsimonious evolutionary trees for the viral capsid proteins (VP1, VP2/VP3) and the large tumor (T) antigen. We show that these three coding sequences can yield phylogenetic trees similar to each other and to that of their host species. Such virus-host "co-speciation" appears incongruent with some prevailing views of viral evolution, and we suggest that inapparent persistent infections may link virus and most host evolution. Similarity analysis identified three specific regions of polyoma regulatory gene products (T antigens) as highly conserved, and two of these regions correspond to binding sites for host regulatory proteins (p53, the retinoblastoma gene product p105, and the related protein p107). The p53 site overlaps with a conserved ATPase domain and the retinoblastoma site corresponds to conserved region 1 of E1A protein of adenovirus type 5. We examined the local conservation of these binding sequences and show that the conserved retinoblastoma binding domain is characteristic and inclusive of the entire polyomavirus family, but the conserved p53-like binding domain is characteristic and inclusive of three entire families of small DNA viruses: polyomaviruses, papillomaviruses, and parvoviruses. The evolution of small-DNA-virus families may thus be tightly linked to host evolution and speciation by interaction with a subset of host regulatory proteins.


Subject(s)
Biological Evolution , Capsid/genetics , DNA Viruses/genetics , Regulatory Sequences, Nucleic Acid , Viral Proteins/genetics , Amino Acid Sequence , Animals , Antigens, Polyomavirus Transforming/genetics , Binding Sites , DNA Viruses/pathogenicity , Humans , Molecular Sequence Data , Phylogeny , Retinoblastoma Protein/genetics , Retinoblastoma Protein/metabolism , Sequence Homology, Amino Acid , Species Specificity , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism , Viral Proteins/metabolism
12.
J Virol ; 67(3): 1424-32, 1993 Mar.
Article in English | MEDLINE | ID: mdl-8382304

ABSTRACT

Kidneys of newborn (but not adult) mice are normally high permissive for polyomavirus (Py) infection and readily establish persistent infections. We have proposed that ongoing cellular differentiation, which occurs in newborn mice, may be necessary for a high level of in vivo Py replication (R. Rochford, J. P. Moreno, M. L. Peake, and L. P. Villarreal, J. Virol. 66:3287-3297, 1992). This cellular differentiation requirement may also be necessary for the reactivation of a persistent Py kidney infection and could provide an alternative to the accepted view that reactivation results from immunosuppression. To examine this proposal, the ability of adult BALB/c mouse kidneys to support primary acute Py infection or to reactivate previously established persistent Py infections after kidney-specific damage was investigated. Kidney damage was induced by both chemical (glycerol, cisplatin, or methotrexate) and mechanical (through renal artery clamping to produce unilateral renal ischemia) treatments. We also examined the effects of epidermal growth factor (EGF), which enhances the rate of kidney regeneration, on Py replication. Using histopathologic techniques, in situ hybridization for Py DNA, and immunofluorescence for Py VP1 production, we established that both chemical damage and damage through renal artery clamping of adult kidneys promoted high levels of primary Py replication in these normally nonpermissive cells. This damage also promoted the efficient reactivation of Py replication from persistently infected kidneys, in the absence of immunosuppression. EGF treatment significantly increased acute Py replication and also reactivation in damaged kidneys. These results support the view that ongoing cellular division and differentiation may be needed both for high levels of acute Py replication and for reactivation of persistent infections in vivo.


Subject(s)
Kidney Tubules/microbiology , Polyomavirus/pathogenicity , Tumor Virus Infections/pathology , Acute Disease , Animals , Capsid/isolation & purification , Capsid Proteins , Chronic Disease , Cisplatin/pharmacology , Epidermal Growth Factor/pharmacology , Epithelium/drug effects , Epithelium/microbiology , Epithelium/pathology , Fluorescent Antibody Technique , Glycerol/pharmacology , Ischemia , Kidney Tubules/drug effects , Kidney Tubules/pathology , Methotrexate/pharmacology , Mice , Mice, Inbred BALB C , Polyomavirus/growth & development , Virus Activation
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