ABSTRACT
For the past two decades, forensic DNA analysis has rapidly expanded in both utility and value to criminal investigations. As the number of crime scene and convict/arrestee samples has continued to grow, many forensic DNA laboratories find themselves struggling to test samples in a timely fashion. Agencies employ various methods for calculating their sample intake and processing capacity, yet database and casework sample backlogs continue to present a major challenge. One issue many forensic laboratories face is limited availability of resources for training new analysts. High-quality training enables analysts to effectively perform various aspects of DNA profiling, and as such, it is essential to ensuring consistent, high-quality results. This is well documented in the guidelines established in the FBI's Quality Assurance Standards for Forensic DNA Testing Laboratories in the United States as well as internationally by agencies like INTERPOL. A facility dedicated to training analysts on both theoretical and practical aspects of automated sample processing accelerates the establishment and expansion of high-throughput forensic DNA laboratories. The present article will discuss various aspects of training and agencies that provide such training programs.
ABSTRACT
Sequence-based gene expression data are used to interpret results from functional genomic and proteomics studies. Although more than 300 000 bovine-expressed sequence tags (ESTs) are available in public databases, a more thorough and directed sampling of the expressed genome is needed to identify new transcripts and improve assembly and annotation of existing transcript sequences. Accordingly, we examined the utility of constructing cDNA libraries synthesized by arbitrarily primed RT-PCR of mRNA from tissues not well represented in the publicly available bovine EST database. A total of 33 cDNA libraries were constructed from healthy and infected mammary gland tissues of Brazilian Gir and Holstein cattle. This series of libraries was used to generate 6481 open reading frame-expressed sequence tags (ORESTES) that assembled into 1798 unique sequence elements of which, 1157 did not significantly match sequence assemblies available in the Bos taurus gene index. However, a total of 264 of these 1157 sequence elements aligned with mouse and human expressed sequences demonstrating that ORESTES is an effective resource for discovery of novel expressed sequences in cattle. Furthermore, comparison of the alignment position of bovine ORESTES-derived sequence elements to human gene reference sequences suggested that the priming events for cDNA synthesis more often occurred at the central portion of a transcript, which may have contributed to the relatively high rate of novel sequence discovery.
