[Effect of Argiope lobata venom and its components on L[3H)glutamate binding with locust muscle membranes]. / Deistvie iada pauka Argiope lobata i ego komponentov na sviazyvanie L[3H]glutamata s membranami myshts saranchi.

The Argiope lobata venom is shown to block synaptic potential at locust neuromuscular junctions and inhibit the high-affinity sodium independent L[3H]glutamate binding site in locust muscle membranes. The data obtained due to fractionation of venom evidence that it contains components which block synaptic potential and inhibit the binding of L[3H]glutamate (5 kDa and more) as well as components which block synaptic potential but do not inhibit the binding of L[3H]glutamate less than 5 kDa. These observations indicate that spider venom contains at least two components with different mechanism of action.

Postsynaptic blocking of glutamatergic and cholinergic synapses as a common property of Araneidae spider venoms.

Venom effects of eight Araneidae spider species were studied using locust and frog neuromuscular junctions. The spider venoms irreversibly blocked miniature excitatory postsynaptic potentials and excitatory postsynaptic potentials of locust neuromuscular junction. The frog miniature end-plate potentials and end-plate potentials were also blocked, but they recovered upon washing of the preparation with physiological solution.