Subject(s)
Nerve Regeneration/physiology , Neurons, Afferent/metabolism , Satellite Cells, Perineuronal/metabolism , Sciatic Nerve/metabolism , Vimentin/metabolism , Adjuvants, Immunologic/pharmacology , Animals , Apoptosis/physiology , Axotomy , Biomarkers , Cell Survival/drug effects , Cell Survival/physiology , Ganglia, Spinal/cytology , Ganglia, Spinal/metabolism , Male , Nerve Regeneration/drug effects , Neurons, Afferent/drug effects , Proto-Oncogene Proteins c-bcl-2/metabolism , Pyrimidines/pharmacology , Rats , Satellite Cells, Perineuronal/drug effects , Sciatic Nerve/drug effects , Sciatic Nerve/injuriesABSTRACT
Using the model of pharmacological stimulation of rat sciatic nerve regeneration, the effect of pyrimidine derivative ximedon on survival of different populations of LIV-LV dorsal root ganglion neurons, their expression of antiapoptotic Bcl-2 protein and number of neurons surrounded by vimentin-positive satellite cell, was studied. 90 days after nerve cutting, ximedon was found to reduce posttraumatic death of neurons having both medium-sized and large perikarya by 35.5% and 42.8%, respectively, to support Bcl-2 expression in all neuronal populations and to modify vimentin expression in satellite cells. After nerve cutting, the number of small and medium-sized neurons with vimentin-expressing satellite cells was significantly increased while the population of large neurons was diminished. Nerve regeneration stimulation by ximedon induced the increase in the number of large neurons, surrounded by vimentin-expressing satellite cells, up to the levels found in intact animals.
Subject(s)
Nerve Regeneration/drug effects , Neurons/cytology , Pyrimidines/pharmacology , Sciatic Nerve/drug effects , Animals , Biomarkers , Cell Survival/drug effects , Ganglia, Spinal/cytology , Ganglia, Spinal/drug effects , Ganglia, Spinal/metabolism , Male , Proto-Oncogene Proteins c-bcl-2/metabolism , Rats , Sciatic Nerve/cytology , Sciatic Nerve/physiology , Vimentin/metabolismABSTRACT
Pyrimidine derivative xymedon inhibits neuronal death in L4-L5 spinal ganglia 30 days after ligation of rat sciatic nerve. After treatment with xymedon the number of neurons on the operated side decreased by 22.1% compared to that on the contralateral side, while in the control group this parameter decreased by 28.7%. At the same terms, the number of Schwann cells on the operated side after xymedon injection increased by 27.7% in comparison with that on the contralateral side, while in the control group this parameter decreased by 57.3%.
