AIEE active sensors for fluorescence enhancement based detection of Ni2+ in living cells: Mechanofluorochromic and photochromic properties with reversible sensing of acid and base.

Stimuli responsive sensors QI 1 and QI 2 were rationally developed which exhibited diverse features of mutable mechanofluorochromism, reversible photochromism, solvatochromism, aggregation induced emission enhancement (AIEE), and metal ion sensing. After observing the exceptional structural property relationship, sensors were applied for reversible colorimetric and fluorometric determination of Ni2+ with low detection limits of 12 and 17 nM, respectively. Fluorescence emission enhancement based Ni2+ sensing was induced by chelation enhanced fluorescence (CHEF) mechanism. CHEF is triggered by the inhibition of excited state intramolecular proton transfer (ESIPT) and -C=N isomerization. The proposed Ni2+ sensing mechanism was investigated through 1H NMR, FT-IR titration, theoretical studies, and Jobs plots. Further, the developed sensors successfully demonstrated the selective acid-base induced absorption/emission switching through reversible ring-opening/closing and keto-enol tautomerization, evidenced by 1H NMR titration experiments. Additionally, the sensitivity of the sensor QI 1 towards Ni2+ was effectively mimicked in live MCF-7 cells and industrial effluents. Furthermore, monitoring of Ni2+ ions was also accessed through inexpensive and portable sensors' coated fluorescent films. Finally, an INHIBIT logic gate was fabricated imputing Ni2+ and EDTA as input signals to electronically scrutinize the targeted Ni2+.

Effect of co-application of wastewater and freshwater on the physiological properties and trace element content in Raphanus sativus: soil contamination and human health.

Nowadays, the use of wastewater for crop irrigation is increasing at global scale mainly due to freshwater scarcity and economic benefits. However, the presence of different types of pollutants including the trace elements (TEs) poses a serious threat to environmental and human health. This pot study evaluated the effect of alone and mixed irrigation water [wastewater (WW) with canal water (CW) and tube-well water (TW)] on TEs build-up in the soil, their soil-plant transfer and allied health hazards in District Vehari. The WW samples were mainly contaminated with Cd (0.03 mg/L), Cr (1.45 mg/L), Cu (0.35 mg/L) and Ni (0.40 mg/L). The CW contained high levels of Cr and Fe, while TW was contaminated with Pb and Cr. In soil, the concentrations of Cd, Fe and Mn exceeded their respective limit values for all the treatments. Among all the treatments, TEs concentration was found highest in WW-3 irrigated soil. Application of all the treatments resulted in TEs (Cu, 60.1 mg/kg; Cd, 8.2 mg/kg; Ni, 39.9 mg/kg; Fe, 4411 mg/kg; Zn, 111.3 mg/kg and Pb, 44.5 mg/kg) accumulation mainly in the edible parts of Raphanus sativus. Compared to other treatments, TW and TW + CW irrigated plants accumulated higher levels of TEs. Results showed linear trends among TEs accumulation and alterations in physiological attributes of R. sativus. High TEs accumulation in TW irrigated treatments (TW + WW-1 and TW + CW) caused maximum H2O2 production, lipid peroxidation and decline in plant pigments. Risk assessment parameters showed both carcinogenic and non-carcinogenic risks for all the irrigation treatments due to high TEs contents in edible tissues. It is concluded that alone or combined application of WW, TW and CW is not fit for vegetable irrigation, in the studied area, due to high TEs contents.

Synthesis, characterization and docking studies of amide ligands as anti-leishmanial agents.

Aim of this study was to synthesize new inhibitors on the basis of active site of aspartic protease enzyme and to evaluate their intended biological activity. A3D model of an enzyme was generated via homology modeling and series of novel amide ligands were synthesized by using a short high yield process, subsequently, analyzed in-silico and in-vitro anti-leishmanial activities. Characterization and identification was accomplished via NMR (H1& C13), infrared and mass spectroscopic techniques. Among all compound (4) was found to show significant activity (IC50 58±0.01) against Leishmania major (L. major) species. Furthermore, docking studies confirmed the inhibition of a targeted enzyme that supported the interaction of potent compound (4) with key residues (aspartic protease) via hydrogen bonds. Present study conferred about novel compound (4) as a promising compound to antagonize L. major activities in future.

Vicissitudes in polyphenolic extract-based high internal phase creams (HIPCs)- effect of storage temperature dependent characteristics.

The purpose of this investigation was to evaluate the vicissitudes in polyphenolic extract- based high internal phase creams (HIPCs) and effect of storage temperature dependent characteristics. Rheological parameters, that is, power law and IPC analysis with its physical characteristics were exploredat different storage temperatures (8°, 25°, 40° and 40° with 75% relative humidity- RH) with different time intervals up to 2 months of newly formulated poly-phenolic extract- based high internal phase cream and its comparison with base. Polyphenolic- based HIPCs showed non-Newtonian-pseudo plastic tendencies in vicissitudes with time and storage temperatures. Data analysis with Power Law and IPC paste was found to fit to all the rheograms. Flow index, shear sensitivity factor, consistency Index and 10 RPM of freshly prepared HIPCs with and without encapsulated polyphenolic extract were found to be 0.5,0.53, 386.4 cP, and 432.9 cP, respectively.The viscosities were fallen with rise in shear stress.There was no change in color, electrical conductivity, liquefaction and phase separation after centrifugation in any sample of polyphenolic extract-based HIPCs and its base. Polyphenolic- based extract HIPCs behaved non-Newtonian- pseudo plastic tendencies and showed stability up to 2 months and can be directed absolutely to shield skin against ultraviolet radiation (UV) intervened oxidative mutilation.

Low temperature conversion of plastic waste into light hydrocarbons.

Advance recycling through pyrolytic technology has the potential of being applied to the management of plastic waste (PW). For this purpose 1 l volume, energy efficient batch reactor was manufactured locally and tested for pyrolysis of waste plastic. The feedstock for reactor was 50 g waste polyethylene. The average yield of the pyrolytic oil, wax, pyrogas and char from pyrolysis of PW were 48.6, 40.7, 10.1 and 0.6%, respectively, at 275 degrees C with non-catalytic process. Using catalyst the average yields of pyrolytic oil, pyrogas, wax and residue (char) of 50 g of PW was 47.98, 35.43, 16.09 and 0.50%, respectively, at operating temperature of 250 degrees C. The designed reactor could work at low temperature in the absence of a catalyst to obtain similar products as for a catalytic process.