Mechanistic Impact of Zinc Deficiency in Human Development.

Zinc (Zn) deficiency in humans is an emerging global health issue affecting approximately two billion people across the globe. The situation prevails due to the intake of Zn deficient grains and vegetables worldwide. Clinical identification of Zn deficiency in humans remains problematic because the symptoms do not appear until impair the vital organs, such as the gastrointestinal track, central nervous system, immune system, skeletal, and nervous system. Lower Zn body levels are also responsible for multiple physiological disorders, such as apoptosis, organs destruction, DNA injuries, and oxidative damage to the cellular components through reactive oxygen species (ROS). The oxidative damage causes chronic inflammation lead toward several chronic diseases, such as heart diseases, cancers, alcohol-related malady, muscular contraction, and neuro-pathogenesis. The present review focused on the physiological and growth-related changes in humans under Zn deficient conditions, mechanisms adopted by the human body under Zn deficiency for the proper functioning of the body systems, and the importance of nutritional and nutraceutical approaches to overcome Zn deficiency in humans and concluded that the biofortified food is the best source of Zn as compared to the chemical supplementation to avoid their negative impacts on human.

Microbial interaction mediated programmed cell death in plants.

Food demand of growing population can only be met by finding solutions for sustaining the crop yield. The understanding of basic mechanisms employed by microorganisms for the establishment of parasitic relationship with plants is a complex phenomenon. Symbionts and biotrophs are dependent on living hosts for completing their life cycle, whereas necrotrophs utilize dead cells for their growth and establishment. Hemibiotrophs as compared to other microbes associate themselves with plants in two phase's, viz. early bio-phase and later necro-phase. Plants and microbes interact with each other using receptors present on host cell surface and elicitors (PAMPs and effectors) produced by microbes. Plant-microbe interaction either leads to compatible or incompatible reaction. In response to various biotic and abiotic stress factors, plant undergoes programmed cell death which restricts the growth of biotrophs or hemibiotrophs while necrotrophs as an opportunist starts growing on dead tissue for their own benefit. PCD regulation is an outcome of plant-microbe crosstalk which entirely depends on various biochemical events like generation of reactive oxygen species, nitric oxide, ionic efflux/influx, CLPs, biosynthesis of phytohormones, phytoalexins, polyamines and certain pathogenesis-related proteins. This phenomenon mostly occurs in resistant and non-host plants during invasion of pathogenic microbes. The compatible or incompatible host-pathogen interaction depends upon the presence or absence of host plant resistance and pathogenic race. In addition to host-pathogen interaction, the defense induction by beneficial microbes must also be explored and used to the best of its potential. This review highlights the mechanism of microbe- or symbiont-mediated PCD along with defense induction in plants towards symbionts, biotrophs, necrotrophs and hemibiotrophs. Here we have also discussed the possible use of beneficial microbes in inducing systemic resistance in plants against pathogenic microbes.

Synthesis, classification and properties of hydrogels: their applications in drug delivery and agriculture.

Absorbent polymers or hydrogel polymer materials have an enhanced water retention capacity and are widely used in agriculture and medicine. The controlled release of bioactive molecules (especially drug proteins) by hydrogels and the encapsulation of living cells are some of the active areas of drug discovery research. Hydrogel-based delivery systems may result in a therapeutically advantageous outcome for drug delivery. They can provide various sequential therapeutic agents including macromolecular drugs, small molecule drugs, and cells to control the release of molecules. Due to their controllable degradability, ability to protect unstable drugs from degradation and flexible physical properties, hydrogels can be used as a platform in which various chemical and physical interactions with encapsulated drugs for controlled release in the system can be studied. Practically, hydrogels that possess biodegradable properties have aroused greater interest in drug delivery systems. The original three-dimensional structure gets broken down into non-toxic substances, thus confirming the excellent biocompatibility of the gel. Chemical crosslinking is a resource-rich method for forming hydrogels with excellent mechanical strength. But in some cases the crosslinker used in the synthesis of the hydrogels may cause some toxicity. However, the physically cross-linked hydrogel preparative method is an alternative solution to overcome the toxicity of cross-linkers. Hydrogels that are responsive to stimuli formed from various natural and synthetic polymers can show significant changes in their properties under external stimuli such as temperature, pH, light, ion changes, and redox potential. Stimulus-responsive hydrogels have a wider range of applications in biomedicine including drug delivery, gene delivery and tissue regeneration. Stimulus-responsive hydrogels loaded with multiple drugs show controlled and sustained drug release and can act as drug carriers. By integrating stimulus-responsive hydrogels, such as those with improved thermal responsiveness, pH responsiveness and dual responsiveness, into textile materials, advanced functions can be imparted to the textile materials, thereby improving the moisture and water retention performance, environmental responsiveness, aesthetic appeal, display and comfort of textiles. This review explores the stimuli-responsive hydrogels in drug delivery systems and examines super adsorbent hydrogels and their application in the field of agriculture.

Macroporous Ceramic Monolith from Nanoparticle-Polyelectrolyte-Stabilized Pickering Emulsions.

In this work, we present a simple and scalable approach for fabricating porous ceramic from emulsions stabilized by a binary mixture of oppositely charged nanoparticles and a polyelectrolyte. The electrostatic heteroaggregation is exploited to form weakly charged particle-polyelectrolyte complexes (PPCs) that readily stabilize oil-in-water emulsions. The concentration of surface-active PPCs is varied to obtain Pickering emulsion gels that can be processed and converted into the macroporous ceramic structure. The polyelectrolyte in the binary mixture not only enables the adsorption of particles to the oil-water interface and renders processability of the emulsions but also acts as a binder. Nearly one-to-one correspondence between the microstructure of the green ceramic obtained after the evaporation of solvents from the gel-like emulsions and the parent emulsions is observed. The green ceramic is further sintered under controlled conditions to obtain a porous ceramic monolith. We demonstrate that the microstructure and the pore size distribution in the final ceramic can be altered by tuning the composition of the individual species used in the emulsion formulation, i.e., by optimization of the particle-polyelectrolyte ratio used in the processing route.

Controlling the microstructure of emulsions by exploiting particle-polyelectrolyte association.

HYPOTHESIS: Albeit solid stabilized emulsions are studied for several decades, the surface of the emulsion drops most often are coated with densely packed and jammed monolayer of particles. However, a control over the area that the particles occupy on the drop surface is necessary, especially in applications involving controlled release of active compounds from emulsions. We hypothesize that it is possible to achieve precise control over the concentration of particles on the surface of emulsions by tailoring the adsorption of different species in a multi-component dispersion used for emulsification. EXPERIMENTS: To this end, we carry out emulsification of oil and aqueous dispersions consisting of a combination of oppositely charged colloidal particles and polyelectrolyte. The droplet size distribution and storage stability of the oil-in-water emulsions, the microstructure, the percentage area of the drop surface occupied by the particles and the adsorption behavior of particle-polyelectrolyte binary dispersions are investigated. FINDINGS: Our results demonstrate that the association between oppositely charged colloidal particles and polyelectrolyte can be exploited to obtain surface active species that aid in the formation of emulsions. Moreover, we found that the concentration of particle-polyelectrolyte complexes and polyelectrolyte in the dispersions used in emulsification greatly influence the mean diameter of the emulsions and their microstructure. Our findings provide a strategy to achieve control over surface coverage of particles on the emulsion droplets across a wide range - from a theoretically possible maximum, ≈90%, to as low as ≈5%. Interestingly, the emulsions formulated are found to possess excellent storage stability irrespective of the particle coverage on the drop surface.

Chitin and its derivatives: Structural properties and biomedical applications.

Chitin, a polysaccharide that occurs abundantly in nature after cellulose, has attracted the interest of the scientific community due to its plenty of availability and low cost. Mostly, it is derived from the exoskeleton of insects and marine crustaceans. Often, it is insoluble in common solvents that limit its applications but its deacetylated product, named chitosan is found to be soluble in protonated aqueous medium and used widely in various biomedical fields. Indeed, the existence of the primary amino group on the backbone of chitosan provides it an important feature to modify it chemically into other derivatives easily. In the present review, we present the structural properties of chitin, and its derivatives and highlighted their biomedical implications including, tissue engineering, drug delivery, diagnosis, molecular imaging, antimicrobial activity, and wound healing. We further discussed the limitations and prospects of this versatile natural polysaccharide.

Doubly pH Responsive Emulsions by Exploiting Aggregation of Oppositely Charged Nanoparticles and Polyelectrolytes.

We present a simple and modular approach to realize highly stable pH responsive Pickering emulsion from mixtures of commercially available oppositely charged nanoparticle and polyelectrolyte. While highly charged nanoparticles and polyelectrolytes when used solely do not stabilize emulsions, we show that the electrostatic attraction between oppositely charged nanoparticles and polyelectrolytes can be exploited to formulate emulsions with long-term stability of up to 8 months. The Ludox CL nanoparticles and poly(4-styrenesulfonate) sodium salt (PSS) when dispersed in aqueous solution at pH 2-11 form particle polyelectrolyte complexes (PPCs) due to heteroaggregation. These complexes are effective in stabilizing oil-in-water Pickering emulsions. We demonstrate that this is due to the formation of weakly charged complexes that are surface active and hence readily adsorbed to the oil-water interface created during emulsification. We show that the composition of nanoparticles and polyelectrolytes in the mixture as well as the pH can be tuned to control the average diameter of the emulsions droplets. Immediate destabilization and doubled responsiveness of the emulsions stabilized by particle polyelectrolyte complexes are illustrated by changing the pH of the stable emulsions formed at intermediate pH to either 1 or 13. The aggregation behavior of nanoparticle-polyelectrolyte mixtures and the effect of various parameters such as mixing fraction, pH, and energy input on the formation of Pickering emulsions is discussed. Furthermore, we show that the formation of near charge neutral aggregates that exhibit optimal wetting conditions is a requirement to accomplish emulsion formation. The visualization of particle polyelectrolyte complexes around the emulsion droplets, their morphology prior to emulsification, and their wetting properties are also investigated to elucidate the mechanism of emulsification.