Subject(s)
Immunoglobulin Fab Fragments/chemistry , Liposomes/chemistry , Maleimides/chemistry , Animals , Biological Transport , Cell Line , Cholesterol , Chromatography, Gel/methods , Electrophoresis, Polyacrylamide Gel/methods , Immunoglobulin Fab Fragments/isolation & purification , Immunoglobulin Fab Fragments/metabolism , Indicators and Reagents , Lipids/chemistry , Liposomes/isolation & purification , Liposomes/pharmacokinetics , Mice , Phosphatidylcholines , RabbitsABSTRACT
Saccharomyces cerevisiae kre5delta mutants lack beta-1,6-glucan, a polymer required for proper cell wall assembly and architecture. A functional and cell biological analysis of Kre5p was conducted to further elucidate the role of this diverged protein glucosyltransferase-like protein in beta-1,6-glucan synthesis. Kre5p was found to be a primarily soluble N-glycoprotein of approximately 200 kDa, that localizes to the endoplasmic reticulum. The terminal phenotype of Kre5p-deficient cells was observed, and revealed a severe cell wall morphological defect. KRE6, encoding a glucanase-like protein, was identified as a multicopy suppressor of a temperature-sensitive kre5 allele, suggesting that these proteins may participate in a common beta-1,6-biosynthetic pathway. An analysis of truncated versions of Kre5p indicated that all major regions of the protein are required for viability. Finally, Candida albicans KRE5 was shown to partially restore growth to S. cerevisiae kre5delta cells, suggesting that these proteins are functionally related.
