ABSTRACT
PURPOSE: Spermatogonial stem cells are affected by the interactions of extrinsic signals produced by components of the microenvironment niche, in addition to the chemical and physical properties of the extracellular matrix. Therefore, this study was initiated to assess the interaction of these cells on a synthetic nanofibrillar extracellular matrix that mimicked the geometry and nanotopography of the basement membrane for cellular growth. METHODS: This study has used a variety of experimental approaches to investigate the interaction of mouse neonatal-derived spermatogonial stem-like cells on a synthetic random oriented three-dimensional nanofibrillar matrix composed of electrospun polyamide nanofibers (Ultra-Web™). RESULTS: Spermatogonial stem-like cell colonies were characterized by their ability to express α6-integrin, Thy-1, PLZF, and ß1-integrin. After culture of cells on the nanofibrillar surfaces for 7 days, the number of colonies, the number of cells in each colony, and the average area of colonies were increased (P < 0.05). However, the expression difference of related markers in both groups was not significant. A significantly higher proliferation and survival was observed in the nanofibrillar group (P < 0.05). After transplantation into the testes of busulfan-treated adult mice, spermatogonial stem-like cell colonies that were cultured on the nanofibrillar surface demonstrated functionality, as verified by their ability to migrate to the seminiferous basal membrane, where they produced additional colonies. CONCLUSIONS: These results have suggested that electrospun nanofibrillar surfaces could provide a more favorable microenvironment for in vitro short term culture of spermatogonial stem-like cell colonies.
Subject(s)
Cell Culture Techniques/methods , Nanofibers/chemistry , Spermatogonia/cytology , Stem Cells/cytology , Animals , Cell Differentiation , Cell Proliferation , Extracellular Matrix/chemistry , Extracellular Matrix/metabolism , Female , Male , Mice , Pregnancy , Stem Cells/metabolism , Surface PropertiesABSTRACT
Genetic parameters and genetic trends for birth weight (BW), weaning weight (WW), 6-month weight (6MW), and yearling weight (YW) traits were estimated by using records of 5,634 Makooei lambs, descendants of 289 sires and 1,726 dams, born between 1996 and 2009 at the Makooei sheep breeding station, West Azerbaijan, Iran. The (co)variance components were estimated with different animal models using a restricted maximum likelihood procedure and the most appropriate model for each trait was determined by Akaike's Information Criterion. Breeding values of animals were predicted with best linear unbiased prediction methodology under multi-trait animal models and genetic trends were estimated by regression mean breeding values on birth year. The most appropriate model for BW was a model including direct and maternal genetic effects, regardless of their covariance. The model for WW and 6MW included direct additive genetic effects. The model for YW included direct genetic effects only. Direct heritabilities based on the best model were estimated 0.15 ± 0.04, 0.16 ± 0.03, 0.21 ± 0.04, and 0.22 ± 0.06 for BW, WW, 6MW, and YW, respectively, and maternal heritability obtained 0.08 ± 0.02 for BW. Genetic correlations among the traits were positive and varied from 0.28 for BW-YW to 0.66 for BW-WW and phenotypic correlations were generally lower than the genetic correlations. Genetic trends were 8.1 ± 2, 67.4 ± 5, 38.7 ± 4, and 47.6 ± 6 g per year for BW, WW, 6MW, and YW, respectively.
