Effect of eco-friendly probiotics-supplemented rapeseed meal-based diet on the performance of Catla catla fingerlings.

Ever-increasing human population compels the researchers to search for alternative food sources such as fish meat. For increase of fish growth and proper feed utilization, probiotics were added in rapeseed meal-based diet in current trial for proper digestion and absorption of nutrients in fish and ultimately higher growth with lower aquatic pollution. Fish gut microbiota is important for the feed utilization and absorption in body for higher growth. A 70-day study was conducted to investigate the effects of probiotics-supplemented rapeseed meal-based diet on growth performance, digestibility of nutrients, and absorption of minerals in Catla catla fingerlings with lowering water pollution level. Six test diets were prepared by using different levels of multi-strain probiotics i.e. 0, 1, 2, 3, 4 and 5 g/kg (0.0-0.5%) in rapeseed meal-based diet. At the rate of 4% of live wet weight, Catla fingerlings were fed two times a day and faeces samples from each tank were collected. According to the results, it was observed that probiotics supplementation (@2 g/kg) in rapeseed meal-based diet resulted with improvement in nutrient digestibility (CP, 72%; fat, 75% and GE, 70%), mineral absorption (Ca, 72%; Na, 76%; K, 70% and P, 70%), specific growth rate (SGR, 1.55), improved feed conversion ratio (FCR, 1.22) and weight gain percentage (WG%, 303%) of fingerlings. It was also noticed that probiotics supplementation decreased the discharge of minerals and nutrient through faeces, as compared to control diet; hence, it plays a significant role in reducing water pollution. On the basis of these results, it was concluded that probiotics inclusion at level of 2 g/kg was useful to formulate the cost effective and eco-friendly fish feed with the maximum improvement in growth and fish health by using rapeseed meal-based diet, as compared to control and other test diets.

Effect of Spore-Forming Probiotics on the Poultry Production: A Review.

Due to the bad aspects associated with the use of antibiotics, the pressure on poultry production prompted the efforts to find out suitable growth-promoting and disease-preventing alternatives. Although many cost-effective alternatives have been developed, currently, one of the most auspicious alternatives for poultry feed is spore-forming probiotics, which can exert more beneficial effects as compared to normal probiotics, because of their ability to withstand the harsh external and internal conditions which result in increased viability. Many studies have already used spore-forming probiotics to improve different parameters of poultry production. Our laboratory has recently isolated a spore-forming bacterial strain, which has the potential to be used as a probiotic. So, to provide a detailed understanding, the current review aimed to collect valuable references to describe the mechanism of action of spore-forming probiotics and their effect on all the key aspects of poultry production.

Efficacy of Probiotics Supplementation on Growth Performance, Carcass Composition and Hematological Parameters of Cyprinus carpio Fingerlings Fed Corn Gluten Meal-Based Diet

Abstract Plant based protein sources are one of the best, cost effective and easily available protein sources being used in fish feed. But due to a lower number of micro-biota in fish gut plant meal based diets cannot be digested and absorbed well in fish body. Probiotics were supplemented at 0, 1, 2, 3, 4 and 5 gkg-1 levels in fish feed for formulating one control and five test diets. In this study, three replicates of each treatment were used and number of fingerlings was 15 in each replicate. The C. carpio (common carp) fingerlings were fed at 5% of live wet weight on their prescribed diet twice daily. The results revealed that supplementation of probiotics in corn gluten meal based diets significantly (p<0.05) improved growth performance, carcass composition and hematological parameters. Most optimum values of growth performance parameters were noted at 2 gkg-1 level of probiotics supplemented diet. C. carpio fingerlings fed corn gluten meal based diet supplemented with 2 gkg-1 level of probiotics indicated significant (p<0.05) improvements in crude protein (17g) crude fat (9g) and gross energy (3 kcalg-1) whereas higher red blood cells (RBCs), white blood cells (WBCs) and hemoglobin (Hb) was also recorded in fish blood when fed 2 gkg-1 probiotics level diet. From these results, it was concluded that 2 gkg-1 probiotics supplementation in corn gluten meal based diet is optimum for improving growth performance, body composition and hematology of C. carpio fingerlings.

Improvement in overall performance of Catla catla fingerlings fed phytase included low cost plant by products-based diet.

Phytic acid's presence in low-cost Moringa by-products effect the availability of important nutrients, diminishing the fish quality and blood composition in fish. Phytate having chelating effects with nutrients and minerals, can be reduced by the supplementation of phytase enzyme. Without the use of enzyme, plant meal may cause water pollution and decrease the fish health that results in higher culture cost. Therefore, current study was designed to check improvement in overall performance of Catla catla fingerlings fed Moringa by product-based diets supplemented with phytase (0, 300, 600, 900, 1200 and 1500, FTU/kg). All diets were integrated with non-digestible marker (Cr2O3) at the rate of 1%. The fingerlings were fed couple of times a day (4% of live wet weight). Results showed significant (p < 0.05) improvement in nutrient digestibility (i.e. EE, CP and GE), carcass composition and hematological parameters (i.e. RBCs, PLT and Hb) at 900, FTU/kg of phytase in contrast with other treatments. Moreover, phytase addition improves the water quality by reducing the nutrients leaching through feces at low cost. Current results indicated that, using mixture of Moringa seed meal and Moringa leaf meal based diet supplemented with phytase at 900, FTU/kg concentration is the most optimum level to develop a cost-effective as well as eco-friendly fish feed with maximum absorption of important nutrients and minerals in fish body resultantly high higher fish performance.

Detection of Exon 12 and 14 Mutations in Janus Kinase 2 Gene Including a Novel Mutant in V617F Negative Polycythemia Vera Patients from Pakistan.

The most frequently reported genetic aberration among polycythemia vera (PV) patients is a gain of function mutation V617F in exon 14 of Janus kinase 2 (JAK2) gene. However in many investigations, V617F negative PV patients have been reported to harbor mutations in JAK 2 exon 12. We investigated 24 patients with PV (diagnosed following 2016 WHO guidelines) to detect V617F mutation through allele specific PCR. The frequency of which was found to be 19/24 (79.2 %). Later on JAK2 exon 12 and 14 was amplified by conventional PCR in V617F negative patients and subjected to sequence analysis. A total of 03 mutated sites in exon 12 were detected in only two V617F-negative patients 2/5 (40%). All three substitutions were heterozygous i.e. F537F/I found in both patients and R528R/T, which is a novel mutation. In addition, one patient 1/5 (10%) manifested amino acid substitution V617A in JAK2 exon 14. Hematological parameters of individuals harboring mutations do not vary significantly than rest of the PV patients. Previous history and 2.3 years of follow-up studies reveal 15-year survival of V617F positive patients (n=19) to be 76%, while it is 94% for wild type V617 patients (n=05). Mean TLC of the patient cohort was 17.6± 9.1 x 109/L, mean platelet count was 552± 253 x 109/L, mean hemoglobin was 16.9± 3.2 g/dl, mean corpuscular volume (MCV) was 77.2± 13.0 fl and mean corpuscular hemoglobin (MCH) was 25.6± 3.9 pg. This is the very first attempt from Pakistan to screen JAK2-exon 12 mutations in PV patients. We further aim to investigate Jak2 exon 12 mutations in larger number of PV patients to assess their clinical relevance and role in disease onset, progression and transformation.