Sustainable soil management under drought stress through biochar application: Immobilizing arsenic, ameliorating soil quality, and augmenting plant growth.

Rise in climate change-induced drought occurrences have amplified pollution of metal(loid)s, deteriorated soil quality, and deterred growth of crops. Rice straw-derived biochars (RSB) and cow manure-enriched biochars (CEB) were used in the investigation (at doses of 0%, 2.5%, 5%, and 7.5%) to ameliorate the negative impacts of drought, improve soil fertility, minimize arsenic pollution, replace agro-chemical application, and maximize crop yields. Even in soils exposed to severe droughts, 3 months of RSB and CEB amendment (at 7.5% dose) revealed decreased bulk density (13.7% and 8.9%), and increased cation exchange capacity (6.0% and 6.3%), anion exchange capacity (56.3% and 28.0%), porosity (12.3% and 7.9%), water holding capacity (37.5% and 12.5%), soil respiration (17.8% and 21.8%), and nutrient contents (especially N and P). Additionally, RSB and CEB decreased mobile (30.3% and 35.7%), bio-available (54.7% and 45.3%), and leachable (55.0% and 56.5%) fractions of arsenic. Further, pot experiments with Bengal gram and coriander plants showed enhanced growth (62-188% biomass and 90-277% length) and reduced arsenic accumulation (49-54%) in above ground parts of the plants. Therefore, biochar application was found to improve physico-chemical properties of soil, minimize arsenic contamination, and augment crop growth even in drought-stressed soils. The investigation suggests utilisation of cow manure for eco-friendly fabrication of nutrient-rich CEB, which could eventually promote sustainable agriculture and circular economy. With the increasing need for sustainable agricultural practices, the use of biochar could provide a long-term solution to enhance soil quality, mitigate the effects of climate change, and ensure food security for future generations. Future research should focus on optimizing biochar application across various soil types and climatic conditions, as well as assessing its long-term effectiveness.

Tracheostomy timing and outcomes in patients with coronavirus disease 2019-associated acute respiratory distress syndrome: A retrospective observational study.

Background: Patients with coronavirus disease 2019 (COVID-19) pneumonitis may progress to acute respiratory distress syndrome (ARDS) requiring endotracheal intubation and prolonged mechanical ventilation (MV). There are limited data on the optimum time of tracheostomy in COVID-19 patients progressing to ARDS. Methods: This was a retrospective observational study of all patients diagnosed with COVID-19 who progressed to ARDS requiring MV and undergone tracheostomy. We aimed to conduct a study to observe the impact of tracheostomy on the mortality of these patients and the impact of timing of tracheostomy on outcomes in these patients. Results: Of the total 162 patients, 128 (79%) were male and 34 (21%) were female. Early group (≤14 days) comprised 37 patients, while 125 patients were included in late group (>14 days). A total of 91 (56%) patients died at the end of this period. Among the patients who died, 21were included in the early group, while the late group comprised the remaining 70 patients. On comparing the patients who died, the duration of stay in the intensive care unit (ICU) was significantly different in the two groups (median [Q1-Q3]: 12 [11-13] vs. 23 [19-28] days, P < 0.001). The number of days to death also differed significantly between the two groups (median [Q1-Q3]: 28 [21-38] vs. 24 [14-30] days, P = 0.009). Conclusion: Early tracheostomy is associated with significantly shorter length of ICU stay in COVID-19 patients that have progressed to ARDS. However, the timing of tracheostomy had no influence on the overall mortality rate in these patients.

Neurological Sequelae After Acute Carbon Monoxide Poisoning.

Carbon monoxide poisoning (COP) is a common cause of death due to poisoning. After COP, a significant number of patients may develop a distinct type of neurological dysfunction called delayed neurological sequel (DNS). Recently, we came across a disaster of COP cases after a fire in a shared accommodation. The hostel was overcrowded and had a faulty air-conditioning/exhaust system. A total of five patients with loss of consciousness and shock were brought to us. They were diagnosed with acute COP based on their history of exposure to carbon monoxide (CO) and elevated carboxyhemoglobin levels in blood gas measurements. All patients were intubated and mechanically ventilated. Standard intensive care management was given to them, which included oxygenation, sedation, fluid resuscitation, and vasopressors. Their carboxyhemoglobin was rapidly reversed with normobaric oxygen therapy (NBO2). Three patients showed good response and neurological recovery after NBO2. Unfortunately, two patients developed DNS. DNS is a neuropsychological condition that may have cognitive, psychiatric, vestibulocochlear, motor, sensory, or diffuse demyelinating effects after COP. DNS is diagnosed in patients with a typical history of exposure to CO and a constellation of signs and symptoms. Neuroimaging, specifically magnetic resonance imaging of the brain with gadolinium contrast, is the method of choice for diagnosis. Treatment of DNS after COP begins with anticipation. All patients should receive appropriate oxygen therapy to bring down carboxyhemoglobin as soon as possible. Hyperbaric oxygen therapy (HBO2) for the treatment of COP and prevention of DNS is still debatable. In the available medical literature, there are conflicting recommendations regarding the use of HBO2 in COP/DNS. Moreover, apart from a lack of consensus, there is also a lack of clarity about optimum timing, duration, atmospheric pressure, and number of sessions of HBO2 in preventing DNS after COP. The development of DNS after COP is not directly responsible for mortality, but recovery sometimes takes a long time, which can contribute to increased morbidity and costs of treatment.

Adsorptive removal of anthracene from water by biochar derived amphiphilic carbon dots decorated with chitosan.

Anthracene belongs to the polycyclic aromatic hydrocarbon (PAH) consisting of benzene rings, unusually highly stable through more π-electrons and localized π-bond in entire rings. Aqueous-phase anthracene adsorption using carbon-based materials such as biochar is ineffective. In this paper, carbon dots (CDs) derived from the acid treatment of coconut shell biochar (CDs/MCSB) decorated with chitosan (CS) are successfully synthesized and applied for anthracene removal from aqueous solutions. The h-CDs/MCSB exhibited fast adsorption of anthracene with significant sorption capacity (Qmax = 49.26 mg g-1) with 95 % removal efficiency at 60 min. The study suggested chemisorption dominated monolayer anthracene adsorption onto h-CDs/MCSB, where a significant role was played by ion-exchange. Density Functional Theory (DFT) suggested the anthracene adsorption was dominated by the electrostatic interactions and delocalized electron, induced by higher polarizability of functional groups on the surface of hybrid CDs/MCSB assisted by chitosan (h-CDs/MCSB). In addition, the aromatic structure of CDs/MCSB and high polarizability of functional groups provided the strong interactions between benzene rings of anthracene and hybrid adsorbent-assisted multiple π-bond through delocalized π-bond and polarization-induced H-bond interactions. The presence of carboxylic and sulfonic groups on the CDs/MCSB surface also contributed to the effective adsorption of anthracene was confirmed by the fluorescence spectra. The results showed that the hybrid adsorbent was an effective material for removing PAHs, usually difficult to remove from water owing to the presence of benzene rings in their structures. Further, consistency in the DFT results suggested the outstanding binding capacity with the anthracene molecules with h-CDs/MCSB.

Potential transmission of SARS-CoV-2 through microplastics in sewage: A wastewater-based epidemiological review.

In light of the current COVID-19 pandemic caused by the virus SARS-CoV-2, there is an urgent need to identify and investigate the various pathways of transmission. In addition to contact and aerosol transmission of the virus, this review investigated the possibility of its transmission via microplastics found in sewage. Wastewater-based epidemiological studies on the virus have confirmed its presence and persistence in both influent sewage as well as treated ones. The hypothesis behind the study is that the huge amount of microplastics, especially Polyvinyl Chloride and Polyethylene particles released into the open waters from sewage can become a good substrate and vector for microbes, especially Polyvinyl Chloride and Polyethylene particles, imparting stability to microbes and aiding the "plastisphere" formation. A bibliometric analysis highlights the negligence of research toward plastispheres and their presence in sewage. The ubiquity of microplastics and their release along with the virus into the open waters increases the risk of viral plastispheres. These plastispheres may be ingested by aquatic organisms facilitating reverse zoonosis and the commercial organisms already reported with accumulating microplastics through the food chain poses a risk to human populations as well. Reliance of high population density areas on open waters served by untreated sewage in economically less developed countries might bring back viral transmission.

KDM6 demethylases integrate DNA repair gene regulation and loss of KDM6A sensitizes human acute myeloid leukemia to PARP and BCL2 inhibition.

Acute myeloid leukemia (AML) is a heterogeneous, aggressive malignancy with dismal prognosis and with limited availability of targeted therapies. Epigenetic deregulation contributes to AML pathogenesis. KDM6 proteins are histone-3-lysine-27-demethylases that play context-dependent roles in AML. We inform that KDM6-demethylase function critically regulates DNA-damage-repair-(DDR) gene expression in AML. Mechanistically, KDM6 expression is regulated by genotoxic stress, with deficiency of KDM6A-(UTX) and KDM6B-(JMJD3) impairing DDR transcriptional activation and compromising repair potential. Acquired KDM6A loss-of-function mutations are implicated in chemoresistance, although a significant percentage of relapsed-AML has upregulated KDM6A. Olaparib treatment reduced engraftment of KDM6A-mutant-AML-patient-derived xenografts, highlighting synthetic lethality using Poly-(ADP-ribose)-polymerase-(PARP)-inhibition. Crucially, a higher KDM6A expression is correlated with venetoclax tolerance. Loss of KDM6A increased mitochondrial activity, BCL2 expression, and sensitized AML cells to venetoclax. Additionally, BCL2A1 associates with venetoclax resistance, and KDM6A loss was accompanied with a downregulated BCL2A1. Corroborating these results, dual targeting of PARP and BCL2 was superior to PARP or BCL2 inhibitor monotherapy in inducing AML apoptosis, and primary AML cells carrying KDM6A-domain mutations were even more sensitive to the combination. Together, our study illustrates a mechanistic rationale in support of a novel combination therapy for AML based on subtype-heterogeneity, and establishes KDM6A as a molecular regulator for determining therapeutic efficacy.

Removal of toxic dye from dye-laden wastewater using a new nanocomposite material: Isotherm, kinetics and adsorption mechanism.

In this study, (hemi)cellulosic biochar-based environment-friendly non-toxic nanocomposite (nAg-AC) was fabricated for an inordinate overlook of toxic dye-laden wastewater depollution. This hybrid nanocomposite grafted with silver nanoparticles, numerous hydroxyl and π-bond containing functional groups exhibited outstanding physicochemical properties. FESEM images indicated the heterogeneous porous structure of nAg-AC, while BET analysis revealed mesoporous property with a significant increment of overall surface area (132%). Imbedding of silver nanoparticles and the presence of multiple hydroxyl groups was evident from the XRD and XPS spectrum. Further, the TGA result indicated excellent thermal stability, and FTIR analysis suggested the involvement of surface functional groups like -OH, =C = O, =NH, =C = C = , and -CH in Rhodamine B (RhB) adsorption. The adsorbent matrix provided the overall mechanical strength and facilitated recycling, while the functional matrix (biochar) provided the adsorptive locus for augmented RhB adsorption efficiency (92.77%). Experiments pertaining to adsorption isotherms and kinetics modeling suggested that RhB was removed through multilayer chemisorption on the heterogeneous nAg-AC surface. The main RhB adsorption mechanism included cumulative efforts of H-bindings, π-π stacking interaction, pore-filling, and electrostatic interactions. The nAg-AC maintained mechanical robustness with significant RhB adsorption even after three consecutive regeneration cycles signifying facile recycling. The nAg-AC displayed an outstanding efficacy for the real industrial wastewater depollution, indicating high effectiveness for practical environmental applications. Finally, the cost analysis (incorporating economic, environmental, and social dimensions) suggested a significant role of the nAg-AC in promoting and establishing sustainable development with the circular economy.

Valorization of fruit waste-based biochar for arsenic removal in soils.

Fruit waste disposal is a serious global problem with only 20% of such waste being routinely treated prior to discharge. Two of the most polluting fruit wastes are orange peel and walnut shell and new methods are urgently required to valorize such waste. In the present study, they where valorized via conversion into biochars at 500 °C (OPB500 for orange peel-based biochar produced at 500 °C and WaSB500 for walnut shell-based biochar produced at 500 °C), and evaluated for arsenic adsorption. A pore-rich surface morphology was observed with a low H/C ratio indicating high stability. Spectroscopic studies revealed the presence of minerals and surface functional groups (amide, carbonyl, carboxyl, and hydroxyl) suggesting high potential for arsenic immobilization. Adsorption studies revealed an arsenic removal efficiency of 88.8 ± 0.04% for WaSB500 exposed to initial arsenic concentration of 8 ppm for 5% biochar dose at 25 °C and 30 min contact time. In comparison, OPB500 showed slightly lower removal efficiency of 80.7 ± 0.1% (10 ppm initial concentration, 5% dose, 25 °C, 90 min contact time). Peak shifts in XRD and FTIR spectra together with isotherm, kinetic, and thermodynamic studies suggested arsenic sequestration was achieved via a combination of chemisorption, physisorption, ion exchange, and diffusion. The present investigation suggests valorization of fruit waste into thermo-stable biochars for sustainable arsenic remediation in dynamic soil/water systems and establishes biochar's importance for waste biomass minimization and metal (loid) removal from fertile soils.

Biochar-based nanocomposite from waste tea leaf for toxic dye removal: From facile fabrication to functional fitness.

The present study utilized discarded tea leaf waste to produce 'Tea leaf biochar' (TLB) as the functional matrix for the fabrication of hybrid nanocomposite (nAg-TC), with colloidal deposition of silver nanoparticles (nAg) via modified chemical co-precipitation, for treatment of dye-laden wastewater. The chemical composition, physicochemical properties, and morphology of nAg-TC, and active surface functional groups involved in adsorption were identified using BET, FESEM-EDX, FTIR, TGA, XPS, and XRD. The nAg-TC matrix was found to be heterogeneous, mesoporous, thermostable, with rich in active surface functional groups (-OH, =NH, =CH, CC, CO, CN, and CC), and nAg as a dopant material. The dye adsorption results indicated the maximum removal efficiency (RhB = 95.89%, CR = 94.10%) at 300 K for rhodamine B (RhB) and Congo red (CR) concentrations of 25 mg L-1 and 22.5 mg L-1, respectively. The present investigation agreed with Freundlich isotherm (R2CR:0.991; R2RhB:0.993) and pseudo-second order kinetic (R2CR:0.999; R2RhB:0.999) model, indicating overall adsorption of RhB and CR through spontaneous and exothermic chemisorption on the heterogeneous surface of nAg-TC. The mechanism of RhB and CR adsorption was complex where nAg-TC, possessing the synergistic effects of TLB and nAg, showed surface complexation, electrostatic attraction, and H-bonding, leading to chemisorption. Study showed excellent reusability of spent nAg-TC, and commendable treatment efficiency for dye-laden real industrial effluents. The study exhibits substantial techno-economic feasibility of adsorbent and translates the principles of circular economy into synthesis of value-added products through sustainable management of biowaste and bioresource.

Fabrication of biochar-based hybrid Ag nanocomposite from algal biomass waste for toxic dye-laden wastewater treatment.

Dual functional innovative approaches were developed to tackle the algal scum problem in water by utilizing the algal (Spirogyra sp.) biomass waste for organic dye-laden industrial wastewater treatment, a global problem, and challenge. Therefore, an algal biochar-based nanocomposite (nAgBC) was synthesized and employed as a low-cost adsorbent for Congo red (CR) removal. Surface morphology, physicochemical characteristics, elemental composition, phase, and stability of the nanocomposite was analyzed using BET, FESEM-EDX, FTIR, XRD, XPS, and TGA. The nanocomposite was found to be thermostable, mesoporous with large and heterogeneous surface area, containing nAg as doped material, where -OH, NH, CO, CC, SO, and CH are the surface binding active functional groups. Maximum adsorption efficiency of 95.92% (18 mg L-1 CR) was achieved (qe = 34.53 mg g-1) with 0.5 g L-1 of nanocomposite after 60 min, at room temperature (300 K) at pH 6. Isotherm and kinetic model suggested multilayer chemisorption, where adsorption thermodynamics indicated spontaneous reaction. Fluorescens spectral analysis of CR confirmed the formation of CR supramolecule, supporting enhanced adsorption. Furthermore, the result suggested a 5th cycle reusability and considerable efficacy towards real textile industrial effluents. Synergistic effects of the active surface functional groups of the biochar and nAg, along with the overall surface charge of the composite lead to chemisorption, electrostatic attraction, H-bonding, and surface complexation with CR molecules. Thus, synthesized nAgBC can be applicable to mitigate the wastewater for cleaner production and environment.

A review of the phytochemical mediated synthesis of AgNP (silver nanoparticle): the wonder particle of the past decade.

Silver nanoparticle (AgNP) has been one of the most commonly used nanoparticles since the past decade for a wide range of applications, including environmental, agricultural, and medical fields, due to their unique physicochemical properties and ease of synthesis. Though chemical and physical methods of fabricating AgNPs have been quite popular, they posed various environmental problems. As a result, the bioinspired route of AgNP synthesis emerged as the preferred pathway for synthesis. This review focuses extensively on the biosynthesis of AgNP-mediated through different plant species worldwide in the past 10 years. The most popularly utilized application areas have been highlighted with their in-depth mechanistic approach in this review, along with the discussion on the different phytochemicals playing an important role in the bio-reduction of silver ions. In addition to this, the environmental factors which govern their synthesis and stability have been reviewed. The paper systematically analyses the trend of research on AgNP biosynthesis throughout the world through bibliometric analysis. Apart from this, the feasibility analysis of the plant-mediated synthesis of nanoparticles and their applications have been intrigued considering the perspectives of engineering, economic, and environmental limitations. Thus, the review is not only a comprehensive summary of the achievements and current status of plant-mediated biosynthesis but also provides insight into emerging future research frontier. Supplementary Information: The online version contains supplementary material available at 10.1007/s13204-021-02135-5.

Performance evaluation of crop residue and kitchen waste-derived biochar for eco-efficient removal of arsenic from soils of the Indo-Gangetic plain: A step towards sustainable pollution management.

Biochar was produced from wheat straw (Triticum aestivum), rice straw (Oryza sativa), and kitchen waste at varying pyrolysis temperatures (300°C-700 °C). The biochars were screened depending on their production and physicochemical properties for the adsorptive removal of arsenic (As). The morphological analysis by Field emission scanning electron microscope revealed a porous biochar surface. Spectroscopic characterization of biochars indicated the co-existence of minerals, carboxyl, carbonyl, amide, and hydroxyl groups, which implies the suitability of biochar to immobilize metal (loid)s from soils. Changes in peaks were observed in Fourier-transform infrared and X-ray diffraction images after As sorption indicating the involvement of chemisorption. The thermogravimetric analysis and a low H/C value derived from the CHNS analyzer confirmed the high stability of biochar. The BET analysis was used to estimate the surface areas of wheat straw (15.8 m2 g-1), rice straw (12.5 m2 g-1), and kitchen waste (2.57 m2 g-1) -derived biochars. Batch sorption studies were performed to optimize experimental parameters for maximum removal of As. Maximum removal of As was observed for wheat straw-derived biochar (pyrolyzed at 500 °C) at 8 mg L-1 initial concentration (IC), 7.5 % dose, 25 °C temperature, and 60 min contact time (83.7 ± 0.06 %); in rice straw-derived biochar (pyrolyzed at 500 °C) at 8 mg L-1 IC, 7.5 % dose, 25 °C temperature, 90 min contact time (83.6 ± 0.37 %); and in kitchen waste-derived biochar (pyrolyzed at 500 °C) at 8 mg L-1 IC, 5 % dose, 25 °C temperature, 60 min contact time (76.7 ± 0.16 %). The sorption model parameters suggested the possibility of chemisorption, physisorption, diffusion, and ion exchange for the removal of As. Therefore, it could be recommended to farmers that instead of disposing or burning straws and waste openly, they could adopt the process of charring to generate livelihood security and mitigation of geogenic contaminants from the soil/water dynamic systems.

Arterial blood pressure is inversely associated with vascular sympathetic reactivity (isometric handgrip exercise) in Gujarati Indian adolescents.

Studies conducted earlier have found that vascular sympathetic reactivity to isometric handgrip exercise is either low or high in adolescents with higher blood pressure (Hypertensives) as compared to adolescents with relatively lower blood pressure (Normotensive). The current study was conducted to determine the correlation of vascular sympathetic reactivity to isometric handgrip exercise with blood pressure in Gujarati Indian adolescents so as to understand the pathogenesis and/consequences of Hypertension in this population. A cross-sectional study was conducted on 651 Gujarati Indian adolescents (285 girls, 366 boys) of age group 13-19 years. Blood pressure was measured by oscillometry and vascular sympathetic reactivity (Percentage rise in Diastolic Blood Pressure, %RDBP) was assessed using isometric handgrip test. Pearson's correlation coefficient was determined to study the correlation between %RDBP and blood pressure. In both girls and boys, %RDBP showed significant negative correlation with resting SBP, DBP and MAP. The study thus indicates that an inverse association exist between arterial blood pressure and vascular sympathetic reactivity to isometric handgrip exercise in Gujarati Indian adolescents.

Design and statistical optimization of osmotically driven capsule based on push-pull technology.

In present investigation attempt was made to develop and statistically optimize osmotically active capsule tailor made from the concept of bilayer (push-pull) osmotic tablet technology. The capsule was comprised of active (drug) and push (osmogen) layer. Active layer was compressed in form of tablet by mixing known amount of drug and formulation excipients. Similarly push layer was made by compressing Mannitol with formulation excipients. Finally, both layers were packed in hard gelatin capsule having small aperture at top and coated with semipermeable membrane to form osmotically active capsule. Formulated and optimized capsules were characterized for Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetric (DSC), scanning electron microscopy, In-vitro drug release study and Release models and kinetics. Statistically optimized formulation showed good correlation between predicted and experimented results, which further confirms the practicability and validity of the model.

Association of sleep duration with blood glucose level of Gujarati Indian adolescents.

Recently studies conducted in various parts of the world indicate short sleep duration as a novel risk factor for development of type 2 diabetes. However, ethnic differences exist in the etiopathogenesis of diseases, the current study was undertaken to study the effect of sleep duration on the blood glucose level of Gujarati Indian adolescents. A randomized, non-experimental, cross-sectional study was done on the voluntary participants n = 332 Gujarati adolescent boys and girls of age group 13-20 years studying at the schools and colleges in the Anand district. The participants were assessed for their sleep duration, body composition and blood glucose level. The sleep duration was reported by the subjects as the number of hours they slept on most of the nights in a week over the last one-year. The observations of the study were then analyzed after grouping them into: 1) Adequate sleep duration at night, ASDN (> or = 7 hrs) and 2) Inadequate sleep duration at night, ISDN (< 7 hrs) groups. One-way ANOVA and post hoc Tuky-Krammer test were used for finding significant differences (P < 0.05) between groups. No significant difference was found in all parameters of body composition and fasting blood glucose level between the ASDN group and ISDN group in both boys and girls. However, gender difference exists in the body composition and blood glucose level. The current study indicates that inadequate sleep duration at night (< 7 hrs) does not affect the blood glucose level of the Gujarati Indian adolescents of age group 13-20 years.

Association of physical activity and physical fitness with blood pressure profile in Gujarati Indian adolescents.

The current study was conducted to determine how physical activity level and physical fitness affects the blood pressure profile of Gujarati Indian adolescents so as to help in developing preventive strategies for the local population as ethnic differences exist in the aetiopathogenesis of hypertension. A cross-sectional study was conducted on 485 Gujarati Indian adolescent boys and girls of age group 16-19 years. Physical activity level was assessed using Johnson Space Center/NASA Physical Activity Rating Scale and VO2 max was used to assess the physical fitness. Body composition was assessed in terms of Body Mass Index, Fat Mass Index and Waist Circumference. Blood Pressure was measured by oscillometry. One-way ANOVA was used to study if any significant differences (P<0.05) existed in the blood pressure profile between the high, moderate and low physical activity groups. Pearson's correlation coefficient was determined to assess the relationship between VO2 max and blood pressure profile. In girls, physical activity level was not found to have a significant effect on the blood pressure profile. In boys, systolic blood pressure and mean arterial pressure were found to be significantly higher in Moderate Physical Activity Group as compared to Low Physical Activity Group. PVO2 max was found to have a significant negative correlationship with SBP, DBP and MAP in girls and a significant negative correlationship with SBP, PP and MAP in boys. It could thus be concluded that a better physical fitness rather than a higher physical activity level could keep the blood pressure in check in the Gujarati Indian adolescents.

Effect of gender on the association of adiposity with cardiovascular reactivity in Gujarati Indian adolescents.

Studies have reported that both gender and adiposity influence cardiovascular reactivity amongst adolescents. However, not much is known about the effect of gender on the association of adiposity with cardiovascular reactivity. The current study was conducted to learn how gender affects the association of adiposity with cardiovascular reactivity in the Gujarati Indian adolescents so as to develop preventive strategies for the local population. A cross-sectional study was conducted on 428 (Girls-173, Boys-255) Gujarati Indian adolescents of age group 16-19 years. Adiposity was assessed in terms of Body Mass Index (BMI), Body Fat Percentage (BF %), Fat Mass (FM), Fat Mass Index (FMI) and Waist Circumference (WC). Percentage Rise in Pulse Rate (%RPR) and Percentage Rise in Diastolic Blood Pressure (%RDBP) during Isometric Handgrip Test were used to assess the cardiovascular reactivity to acute sympathetic stress. Pearson's correlation coefficient was determined to find the association of adiposity with cardiovascular reactivity. Boys were found to have a significantly larger WC, higher physical fitness and greater %RDBP in comparison to girls while girls had a significantly higher BF% and FMI in comparison to boys. In both boys and girls, BMI, BF%, FMI and WC showed significant positive association with %RDBP with stronger correlationship found in girls. Girls also showed a significant negative correlationship between physical fitness and vascular reactivity. It could thus be concluded that gender affected the association of adiposity with vascular reactivity in Gujarati Indian adolescent such that girls tend to have a larger correlationship between adiposity and vascular reactivity than boys which is most likely due to lower physical fitness.