ABSTRACT
Trigonelline-loaded water-soluble chitosan nanoparticles (Trigo-WSCS NPs) were prepared for the treatment of glioblastoma (targeting C6 glioma cells) and also evaluated its biocompatibility with rat adrenal pheochromocytoma cells (PC12 cells). WSCS-Trigo NPs characteristics were determined using UV-Visible spectrophotometer, FTIR, XRD, TEM, DLS, and Zeta potential. Trigo-WSCS NPs were noted to have a spherical shape, with an average size of 356 nm. Trigo-WSCS NPs zeta potential was 30.9 mv, which expresses its good stability. The WSCS-Trigo NPs considerably inhibited the growth of rat C6 glioma cells and exhibited an IC50 concentration of 34 µg/mL. Further, Trigo-WSCS NPs were biocompatible with PC12 cells in terms of enhancing neurite growth and differentiation. In conclusion, Trigo-WSCS NPs could act as an antitumor drug for the treatment of glioblastoma as suggested by the in vitro studies.
Subject(s)
Alkaloids/chemistry , Alkaloids/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Chitosan/chemistry , Drug Carriers/chemistry , Nanoparticles/chemistry , Adrenal Gland Neoplasms/metabolism , Alkaloids/administration & dosage , Animals , Antineoplastic Agents/administration & dosage , Apoptosis/drug effects , Cell Cycle/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Chitosan/administration & dosage , Drug Carriers/administration & dosage , Drug Carriers/chemical synthesis , Drug Liberation , Flow Cytometry , Glioblastoma/drug therapy , Glioblastoma/metabolism , Glioblastoma/pathology , Glioma/drug therapy , Glioma/metabolism , Glioma/pathology , Materials Testing , Microscopy, Fluorescence , Nanoparticles/administration & dosage , Nanoparticles/analysis , Particle Size , Pheochromocytoma/metabolism , RatsABSTRACT
A nanopesticide formulation was developed using chitosan and a botanical pesticide PONNEEM® and its antifeedant, larvicidal and growth regulating activities were screened against Helicoverpa armigera, a major lepidopteran pest. Chitosan nanoparticles (CSNs) were prepared by using two different cross-linking agents namely glutaraldehyde (GLA) and tripolyphosphate (TPP). The effects of cross linking agents on CSNs and the biological properties against the insect pest were also studied. Cross linking of chitosan with either TPP or GLA was confirmed through Fourier Transform Infrared (FTIR) spectroscopy. Electron micrograph revealed that the size of CSNs varied from 32 to 90nm. The stability of nanoparticles lasted for 9days in CSNs-TPP-PONNEEM. In CSNs-GLA-PONNEEM, the stability of nanoparticles was higher. CSNs-TPP-PONNEEM treatment recorded 88.5% antifeedant activity and 90.2% larvicidal activity against H. armigera. Weights of H. armigera pupae in CSNs-TPP-PONNEEM treatment were significantly low. Chitosan-based nano-pesticide formulation holds great promise in H. armigera management.
