Human-induced sediment degradation of Burullus lagoon, Nile Delta, Egypt: Heavy metals pollution status and potential ecological risk.

Heavy metals contamination level and their ecological risk of the Burullus lagoon were estimated using four cores and twelve surficial sediment samples. The distributions maps of Fe, Mn, Zn, Hg, Cu, and Pb indicate an increase toward drains areas, whereas Cd rises toward the Boughaz El-Burullus. The geoaccumulation index (Igeo) and contamination factor (CF) for heavy metal displayed the following order: Cd > Zn > Fe > Cu > Pb > Mn > Hg. The degree of contamination (Cd) indicates a considerable degree of contamination for 81.25% of the studied stations, and the pollution load index (PLI) suggested deterioration in 100% of sediments. Regarding the potential ecological risk (RI), the metals were arranged as: Cd > Hg > Cu > Pb > Zn, with considerable risk at the eastern part. According to sediment quality guidelines (SQGs), Zn concentrations suggest frequently adverse impacts on biota while Cu and Cd indicating an occasional adverse impact. Periodic monitoring of heavy metals in aquatic organisms is recommended to assess their toxic risk.

Biochemical Properties of Tyrosinase from Aspergillus terreus and Penicillium copticola; Undecanoic Acid from Aspergillus flavus, an Endophyte of Moringa oleifera, Is a Novel Potent Tyrosinase Inhibitor.

Tyrosinase is a copper-containing monooxygenase catalyzing the O-hydroxylation of tyrosine to 3,4-dihydroxyphenylalanine then to dopaquinone that is profoundly involved in melanin synthesis in eukaryotes. Overactivation of tyrosinase is correlated with hyperpigmentation that is metabolically correlated with severe pathological disorders, so, inhibition of this enzyme is the most effective approach in controlling the overproduction of melanin and its hazardous effects. Thus, searching for a powerful, selective inhibitor of human tyrosinase to limit the hyper-synthesis of melanin is a challenge. Unlike the difficulty of overexpression of human tyrosinase, using fungal tyrosinase as a model enzyme to the human one to evaluate the mechanistics of enzyme inhibition in response to various compounds is the most feasible strategy. Thus, the purification of highly catalytic-efficient fungal tyrosinase, exploring a novel inhibitor, and evaluating the mechanistics of enzyme inhibition are the main objectives of this work. Aspergillus terreus and Penicillium copticola were reported as the most potential tyrosinase producers. The biochemical properties suggest that this enzyme displays a higher structural and catalytic proximity to human tyrosinase. Upon nutritional bioprocessing by Plackett-Burman design, the yield of tyrosinase was increased by about 7.5-folds, compared to the control. The purified tyrosinase was strongly inhibited by kojic acid and A. flavus DCM extracts with IC50 values of 15.1 and 12.6 µg/mL, respectively. From the spectroscopic analysis, the main anti-tyrosinase compounds of A. flavus extract was resolved, and verified as undecanoic acid. Further studies are ongoing to unravel the in vivo effect and cytotoxicity of this compound in fungi and human, that could be a novel drug to various diseases associated with hyperpigmentation by melanin.

Towards a portable-noninvasive blood pressure monitoring system utilizing the photoplethysmogram signal.

Blood pressure (BP) responds instantly to the body's conditions, such as movements, diseases or infections, and sudden excitation. Therefore, BP monitoring is a standard clinical measurement and is considered one of the fundamental health signs that assist in predicting and diagnosing several cardiovascular diseases. The traditional BP techniques (i.e. the cuff-based methods) only provide intermittent measurements over a certain period. Additionally, they cause turbulence in the blood flow, impeding the continuous BP monitoring, especially in emergency cases. In this study, an instrumentation system is designed to estimate BP noninvasively by measuring the PPG signal utilizing the optical technique. The photoplethysmogram (PPG) signals were measured and processed for ≈ 450 cases with different clinical conditions and irrespective of their health condition. A total of 13 features of the PPG signal were used to estimate the systolic and diastolic blood pressure (SBP and DBP), utilizing several machine learning techniques. The experimental results showed that the designed system is able to effectively describe the complex-embedded relationship between the features of the PPG signal and BP (SBP and DBP) with high accuracy. The mean absolute error (MAE) ± standard deviation (SD) was 4.82 ± 3.49 mmHg for the SBP and 1.37 ± 1.65 mmHg for the DBP, with a mean error (ME) of ≈ 0 mmHg. The estimation results are consistent with the Association for the American National Standards of the Association for the Advancement of Medical Instrumentation (AAMI) and achieved Grade A in the British Hypertension Society (BHS) standards for the DBP and Grade B for the SBP. Such a study effectively contributes to the scientific efforts targeting the promotion of the practical application for providing a portable-noninvasive instrumentation system for BP monitoring purposes. Once the BP is determined with sufficient accuracy, it can be utilized further in the early prediction and classification of various arrhythmias such as hypertension, tachycardia, bradycardia, and atrial fibrillation (as the early detection can be a critical issue).

Simultaneous determination of piracetam and vincamine by spectrophotometric and high-performance liquid chromatographic methods.

A mixture of piracetam and vincamine was determined by 3 different methods. The first was the determination of piracetam and vincamine using the ratio-spectra first-derivative (DD1) spectrophotometric technique at 209 and 293 nm in concentration ranges of 10-45 and 2-14 microg/mL with mean recoveries of 99.22 +/- 0.72 and 99.67 +/- 0.79%, respectively. The second method was based on the resolution of the 2 components by bivariate calibration depending on a mathematic algorithm that provides simplicity and rapidity. The method depended on quantitative evaluation of the absorbencies at 210 and 225 nm in concentration ranges of 5-45 and 2-14 microg/mL, with mean recoveries of 100.33 +/- 0.54 and 100.44 +/- 0.98% for piracetam and vincamine, respectively. The third method was reversed-phase liquid chromatography using 0.05 M potassium dihydrogen phosphate-methanol (50 + 50, v/v) as the mobile phase, with the pH adjusted to 3.5 with phosphoric acid. The eluent was monitored at 215 nm in concentration ranges of 5-100 and 2-200 microg/mL, with mean recoveries of 99.62 +/- 0.67 and 99.32 +/- 0.85% for piracetam and vincamine, respectively. The suggested procedures were checked using laboratory-prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparation. The methods retained their accuracy and precision when applying the standard addition technique. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the manufacturer's method.