Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 6 de 6
Filter
Add more filters










Publication year range
1.
Acta Anatomica Sinica ; (6): 132-136, 2010.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-401823

ABSTRACT

Objective To investigate the expression of tumor suppressor gene p16~(INK4A) in mouse endometrium during the estrus cycle and early pregnancy and its possible role in blastocyst implantation. Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of p16~(INK4A) mRNA,immunohistochemistry and Western blotting were applied to detect p16~(INK4A) protein in mouse endometrium tissues during the estrus cycle and early pregnancy. Results The intensity of p16~(INK4A) mRNA expression in mouse early pregnancy was higher than that in the estrus cycle.Compared with the other 3 stages, the level of p16~(INK4A) mRNA expression at estrus was obviously higher. During the early pregnancy, the level of p16~(INK4A) mRNA expression increased steadily from day 2 to day 5,reaching the maximal level on day 5,then decreasing. Both immunohistochemical and Western blotting analysis showed the same results in expression patterns of p16~(INK4A) protein for mouse endometrium tissues as those results of RT-PCR.Conclusion p16~(INK4A) is involved in the embryos penetrating into the endometrial barrier.

2.
Cancer Genet Cytogenet ; 168(2): 133-45, 2006 Jul 15.
Article in English | MEDLINE | ID: mdl-16843103

ABSTRACT

We report a series of 43 consecutive therapy-related myelodysplastic syndromes (t-MDS) or acute myeloid leukemias (t-AML) observed for 6 years. This series consisted of 26 women and 17 men, ages ranging from 9 to 85 years. These cases were classified into three groups according to the primary diagnosis. Conventional cytogenetic and fluorescent in situ hybridization (FISH)/ multiplex FISH (M-FISH) methods were used to analyze cytogenetic characteristics of secondary MDS/AML. The features of chromosomal abnormalities were linked to the nature of the therapy and protocols used. A considerable proportion of recurrent balanced translocations characterized t-AML secondary to therapy. FISH techniques showed that conventional cytogenetics often underestimated associated translocations; some deletions were in fact derivative chromosomes associated with deletions. After treatment for lymphomas and chronic myeloproliferative diseases, there were more complex unbalanced abnormalities than the control group. Compared to other series, recurrent translocations appeared to be more numerous (25%), probably reflecting an evolution of therapeutic modalities.


Subject(s)
In Situ Hybridization, Fluorescence/methods , Leukemia, Myeloid, Acute/genetics , Myelodysplastic Syndromes/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Gene Amplification , Gene Deletion , Humans , Karyotyping , Male , Middle Aged , Translocation, Genetic
3.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-574240

ABSTRACT

Objective:To analyze the situation of high risk behaviors and other STI/AIDS factors among men who have sex with men(MSM)by examining the situation in a city of southwest China.Methods:A total of 267 homosexual and bisexual men were surveyed anonymously,and the results were analyzed with SPSS.Results:There was a common phenomenon during the process of MSM behaviors that numbers of MSM had more than one sexual partner and unprotected intercourse,which resulted in 21.35% incidence of STI in recent one year.Conclusion:High prevalence of STI and risky behaviors related to AIDS exist among MSM behavior.Thus,it is in urgent need to cease STI/AIDS transmission in MSM and other groups.

4.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-562468

ABSTRACT

Objective To investigate the effect of PTEN gene in the mouse uterus during embryo implantation.Methods Real-time fluorescence quantitative PCR(FQ-PCR) was used to detect PTEN mRNA expressed in the endometria of the nonpregnant mice and the late pregnant mice(day 1,day 3,day 4,day 5 and day 7),with 20 mice sacrificed at each fixed day.Out of another 20 3-day pregnant mice,ten received PTEN antisense oligonucleotide at the horn of uterus and ten received normal saline to count the blastocysts at pregnant day 8.Results The PTEN mRNA/?-actin mRNA in pregnant mice was higher than that of nonpregnant mice,gradually hoisted as days passed by,and reached the highest at pregnant day 5.The number of blastocysts in the mice that received PTEN antisense oligonucleotide was fewer than that received normal saline.Conclusion PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in the regulation process of mouse blastodyst implantation.

5.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-622764

ABSTRACT

With the wide spread of sex and reproductive health knowledge and the deep influence of various cultures,there has been made rapid progress in the study and spread of sex knowledge in our country.It is quite necessary to add courses on sexology in medical higher schools.After a systematic study,students will master the related medical knowledge about sex in a full and scientific way,which will help them lay a solid foundation in their future study of sexology,gynecology and obstetrics,male science and family planning and carry out effective clinical diagnosis treatment for the patients with sex and related diseases.

6.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-622587

ABSTRACT

Since the teaching and research of sexual medicine in our country is conducted for not a long time,the available materials and information about it are not enough.In order to improve the quality of sexual medicine teaching and accumulate teaching experience,based on the teaching practice of sexual medicine in our school in the past,a brief summary and analysis about the teaching purpose,subject design,and the teaching method and effect of sexual medicine in our school are reviewed in the present article.

SELECTION OF CITATIONS
SEARCH DETAIL
...