ABSTRACT
Antinociceptive activity of dalargin (7.5 mg/kg) adsorbed on poly(butyl)cyanoacrylate nanoparticles with different coating was studied on outbred albino mice by the tail-flick test. poly(butyl)cyanoacrylate nanoparticles without coating did not increase the antinociceptive activity of dalargin and hence, did not increase its transport across the blood-brain barrier. poly(butyl)cyanoacrylate nanoparticles coated with apolipoprotein B, apolipoprotein E, and polysorbate 80 increased the transport of dalargin across the blood-brain barrier. Delivery of dalargin to the brain was most effective in case of using poly(butyl)cyanoacrylate nanoparticles with polysorbate 80 coating and subsequent supercoating with apolipoprotein E.
Subject(s)
Analgesics/pharmacology , Apolipoproteins B/pharmacokinetics , Apolipoproteins E/pharmacokinetics , Blood-Brain Barrier/drug effects , Enkephalin, Leucine-2-Alanine/analogs & derivatives , Adsorption , Animals , Animals, Outbred Strains , Apolipoproteins B/administration & dosage , Apolipoproteins E/administration & dosage , Behavior, Animal/drug effects , Biological Transport/drug effects , Biological Transport/physiology , Blood-Brain Barrier/physiology , Coated Materials, Biocompatible/chemistry , Drug Carriers , Drug Delivery Systems/methods , Enbucrilate/chemistry , Enkephalin, Leucine-2-Alanine/pharmacology , Mice , Nanoparticles/chemistry , Particle Size , Polysorbates/administration & dosage , Polysorbates/pharmacokinetics , Time FactorsABSTRACT
Reparative osteogenesis was studied after xenotransplantation of suspension cell graft from human mesenchymal stem cells. A model of experimental damage to rat femoral diaphysis was developed. The state of animals was satisfactory and non-depressed in the early and late postoperation period. We revealed no local pathological reactions and complications. Administration of mesenchymal stem cells into the area of bone defect accelerated and improved regeneration. Unilateral transplantation of the cell graft stimulated regeneration in the contralateral limb due to acceleration of bone tissue maturation. On day 90 after treatment the bone regenerate was completely developed in the area of defect in animals of various groups. The newly formed bone tissue was well integrated into the bone organ.
Subject(s)
Femur/injuries , Mesenchymal Stem Cell Transplantation , Osteogenesis/physiology , Regeneration/physiology , Wound Healing/physiology , Animals , Diaphyses/injuries , Histological Techniques , Humans , Rats , Transplantation, HeterologousABSTRACT
Regeneration of the bone tissue after unilateral xenogeneic transplantation of a suspension of human mesenchymal cells and chondroblasts was studied in rats with experimental damage to both femurs. The state of animals was satisfactory and non-depressed in the early and late postoperation period. No local pathological reactions and complications were seen. Administration of mesenchymal stem cells and chondroblasts into the area of bone defect accelerated regeneration on days 10 and 30 (compared to the control group). Implantation of mesenchymal stem cells more significantly stimulated reparative osteogenesis compared to treatment with chondroblasts. This was mainly associated with increased ratio of mature lamellar bone tissue. Unilateral transplantation of the cell suspension stimulated regeneration in the contralateral limb due to accelerated maturation of the bone tissue. The bone tissue formed after transplantation of mesenchymal stem cells and chondroblasts was integrated into bone organ and underwent complete remodeling. Xenotransplantation of prenatal mesenchymal stem cells and chondroblasts without immunosuppression was not followed by the development of early and delayed complications or local reactions of graft rejection.
