[The mutagenic effect of plasmid DNA pUC19 and its derivatives]. / Mutagennyi éffekt plazmidnykh DNK pUC19 i ee proizvodnykh.

For a better understanding of the mechanism of foreign DNA mutagenesis, it is essential to study the validity of the changing DNA structure for manifestation of its mutagenic action on Drosophila melanogaster (method Basc). The testing of three different kinds of plasmid DNA has been carried out. The injections of only plasmid DNA pUC18 lead to a significant increase in the X-linked recessive lethal frequency. It is important to check mutagenicity of every new recombinant DNA.

[Transposition and reversion of mutations induced in Drosophila by viral DNA]. / Transpozitsii i reversii mutatsii drozofily, indutsirovannykh virusnoi DNK.

Injection of solutions of highly polymerized DNA isolated from nuclear polyhedrosis virus of Galleria mellonella into adult males induced with a considerable frequency visible mutations, two of which were studied in detail. They were detected in about 30 000 flies in the progeny of treated males. Much less than Notched-wings much greater than (Ndw, chromosome 3, location 87.9, dominant) independently arose 12 times, much less than thickened-veins much greater than (thi, chromosome 2, location 71.4, recessive) independently arose 7 times. No mutations were detected in the control of the same size. It was found that both Ndw and thi mutations gave frequent transpositions and reversions in mature, immature germ cells and in somatic cells, in latter cases leading to mosaicism. These results demonstrate for the first time that mutations induced by exogenous DNA are capable of transposition and reversion.