ABSTRACT
In this paper, the cathodic microarc plasma electrolysis (CMPE) was tentatively performed to degrade the phenol in aqueous solution by vapor-gaseous envelope discharge around the Ti cathode. Degradation efficiency of phenol was measured and the intermediate products were evaluated. The suspended particles in solution were analyzed and the decomposition mechanism of phenol in the process of cathodic microarc plasma degradation was discussed. The instantaneous current efficiency reached a peak value firstly and then decreased with the discharge time. In the cathodic plasma region, the Cl substituted the hydrogen on benzene ring in the initial time, which was replaced by a hydroxyl immediately, then the polyhydroxy phenol was easily decomposed. In addition, optical emission spectroscopy was carried out to characterize the plasma features during the CMPE process. The temperature of electron around the microarc discharge envelope reached about 4000â K; thus the phenol was partly carbonized to become graphite particles. CMPE is a promising approach to degrade quickly the phenol in wastewater.
Subject(s)
Phenol , Wastewater , Electrochemistry , Electrodes , Electrolysis , Oxidation-Reduction , PhenolsABSTRACT
Phytochrome B (phyB) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. In this study, we isolate a full-length cDNA of PHYB, designated BrPHYB, from Chinese cabbage (Brassica rapa L. ssp. pekinensis), and we find that BrphyB protein has high amino acid sequence similarity and the closest evolutionary relationship to Arabidopsis thaliana phyB (i.e., AtphyB). Quantitative reverse transcription (RT)-PCR results indicate that the BrPHYB gene is ubiquitously expressed in different tissues under all light conditions. Constitutive expression of the BrPHYB gene in A. thaliana significantly enhances seedling de-etiolation under red- and white-light conditions, and causes dwarf stature in mature plants. Unexpectedly, overexpression of BrPHYB in transgenic A. thaliana resulted in reduced expression of gibberellins biosynthesis genes and delayed flowering under short-day conditions, whereas AtPHYB overexpression caused enhanced expression of FLOWERING LOCUS T and earlier flowering. Our results suggest that BrphyB might play an important role in regulating the development of Chinese cabbage. BrphyB and AtphyB have conserved functions during de-etiolation and vegetative plant growth and divergent functions in the regulation of flowering time.
Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Brassica rapa/genetics , Etiolation/genetics , Gene Expression Regulation, Developmental , Phytochrome B/genetics , Arabidopsis/growth & development , Arabidopsis/radiation effects , Arabidopsis Proteins/metabolism , Base Sequence , Flowers/genetics , Flowers/growth & development , Flowers/radiation effects , Gene Expression , Gene Expression Regulation, Plant , Gibberellins/metabolism , Light , Molecular Sequence Data , Organ Specificity , Phylogeny , Phytochrome B/metabolism , Plant Growth Regulators/metabolism , Plant Leaves/genetics , Plant Leaves/growth & development , Plant Leaves/radiation effects , Plant Proteins/genetics , Plant Proteins/metabolism , Plants, Genetically Modified , Seedlings/genetics , Seedlings/growth & development , Seedlings/radiation effects , Sequence Analysis, DNA , Time FactorsABSTRACT
Mixotrophic cultivation is one potential mode for microalgae production, and an economically acceptable and environmentally sustainable organic carbon source is essential. The potential use of xylose for culturing Scenedesmus obliquus in a mixotrophic mode and physiological features of xylose-grown S. obliquus were studied. S. obliquus had a certain xylose tolerance, and was capable of utilizing xylose for growth. At a xylose concentration of 4gL(-1), the maximal cell density was 2.2gL(-1), being 2.9-fold of that under photoautotrophic condition and arriving to the level of mixotrophic growth using 4gL(-1) glucose. No changes in cellular morphology of the cells grown with or without xylose were detected. Fluorescence emission from photosystem II (PS II) relative to photosystem I (PS I) was decreased in mixotrophic cells, implying that the PSII activity was decreased. The biomass lipid content was enhanced and carbohydrate concentration was decreased, in relation to photoautotrophic controls.
