Knowledge, Attitude, and Practice About Antibiotic Use and Antimicrobial Resistance Among Nursing Students in China: A Cross Sectional Study.

Purpose: The knowledge, attitude, and practices (KAP) concerning antibiotics by healthcare students have the potential impact on controlling antibiotic abuse and antimicrobial resistance (AMR) growth. This study aims to evaluate the levels and explore the associated factors with KAP on antibiotic use and AMR in Chinese nursing students. Methods: A cross-sectional survey using a self-administered questionnaire consisting of demographics and selected features and KAP on antibiotic use and AMR was conducted to measure KAP levels among nursing students at various universities in Hubei Province, China. The logistic regression analyses were performed to analyze the potential factors associated with the KAP. Results: The survey eventually included a total of 1959 nursing students. The mean scores for KAP were 57.89 ±26.32, 55.00 ±12.50, and 71.88 ±15.63, respectively. Regarding knowledge, 54.3% of participants were unaware that antibiotic was ineffective against viral infections. Regarding attitude, 36% of participants agreed that current antibiotic abuse existed; 96.2% of participants thought it necessary to set up a special course on antibiotics. Regarding practice, only 48.4% of participants usually purchased antibiotics with a prescription. Multivariable analyses indicated that lack of discussion on AMR in school courses was an independent risk factor against KAP, respectively. The main knowledge sources of antibiotic being outside the classroom was an independent risk factor related to knowledge and practice. The average score >80 points was an independent protective factor related to knowledge and practice. Conclusion: The KAP level on antibiotic use and AMR among Hubei nursing students was general and required further strengthening. Nursing students with risk factors should be prioritized in educational interventions. The findings of our study pointed out some directions for tailored interventions to improve the training on antibiotics.

MiR-223-3p affects the proliferation and apoptosis of HCAECs in Kawasaki disease by regulating the expression of FOXP3.

OBJECTIVE: Kawasaki disease (KD) can lead to permanent damage to coronary structures, the pathogenesis of which remains unknown. This experiment was designed to investigate whether miR-223-3p secreted in the serum of KD patients affects the proliferation and apoptosis of HCAECs in KD by regulating FOXP3. METHODS: Blood samples were collected in acute febrile phase of KD, after IVIG treatment, and from healthy controls. Transfected into HCAECs cells by synthetic FOXP3 siRNA/NC. A co-culture system was established between HCAECs cells transfected with FOXP3 siRNA/NC and THP1 cells added with three sera. RESULTS: Compared with the control group, the expressions of miR-223-3p, RORγt, and Th17 in serum of KD patients were significantly upregulated, and the expressions of TGF-ß1, FOXP3 and Treg were significantly downregulated. At the same time, the levels of IL-6, IL-17, and IL-23 were significantly increased, and the levels of IL-10 and FOXP3 were significantly decreased. After IVIG treatment, the patient's above results were reversed. The serum of KD patients increased the expression of miR-223-3p and inhibited the expression of FOXP3 in HCAECs cells. IVIG serum is the opposite. Overexpression of miR-223-3p also promoted the apoptosis of HCAECs. In addition, serum from KD patients promoted apoptosis, whereas serum after IVIG treatment inhibited apoptosis. KD patient serum downregulated the expression of FOXP3, Bcl2, TGF-ß1 and IL-10 in cells, and upregulated the expression of caspase3, Bax, IL-17, IL-6, and IL-23. The opposite results were obtained with IVIG-treated sera. CONCLUSION: miR-223-3p secreted in serum of KD patients can regulate the expression of FOXP3 and affect the proliferation, apoptosis, and inflammation of cells.

Kinetics of Catalytic Oxidation of Methylene Blue with La/Cu Co-Doped in Attapulgite.

Methylene blue (MB) is a common pollutant in wastewater from the printing and dyeing industries. In this study, attapulgite (ATP) was modified with La3+/Cu2+, using the method of equivolumetric impregnation. The La3+/Cu2+ -ATP nanocomposites were characterized using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The catalytic properties of the modified ATP and the original ATP were compared. At the same time, the influence of the reaction temperature, concentration of methylene blue and pH on the reaction rate were investigated. The optimal reaction conditions are as follows: MB concentration is 80 mg/L, the dosage of the catalyst is 0.30 g, the dosage of hydrogen peroxide is 2 mL, the pH is 10 and the reaction temperature is 50 °C. Under these conditions, the degradation rate of MB can reach 98%. The recatalysis experiment was carried out reusing the catalyst, and the experimental results showed that the degradation rate could reach 65% after three uses, indicating that the catalyst could be recycled many times and costs could be reduced. Finally, the degradation mechanism of MB was speculated, and the reaction kinetic equation was obtained as follows: -dc/dt = 14,044 exp(-3598.34/T)C(O)0.28.

Safety culture system construction in coal mine enterprises from the perspective of hazards: A case study on the Yimei Coal Group.

BACKGROUND: The construction of a safety culture in coal mine enterprises is an essential component of coal mine safety management. Current studies do not address the mechanism for forming and constructing systems for coal mine enterprise safety culture, and many studies are based on imperfect theoretical frameworks and unsystematic empirical research, their policy recommendations are not systematic or operable, and they offer no feasible safety culture construction system. OBJECTIVE: This study is devoted to analysing the theoretical basis of safety culture construction in coal mine enterprises from the perspective of hazards and proposing the content, stages and targets of constructing a safety culture and develops pragmatic approaches for coal mining enterprises to improve safety culture. METHODS: A theoretical basis for safety culture construction in coal mine enterprises is proposed from the perspective of hazards by considering accident-causing mechanisms based on hazards. Furthermore, this study applied the case analysis and application to conduct empirical research on the proposed theoretical basis for safety culture construction from the perspective of hazards. RESULTS: Four aspects are proposed to capture the content and objectives of safety culture construction from the perspective of hazards: safety concept, behaviour safety, material state safety, and safety institutions. Furthermore, this paper provides a case study of safety culture construction by the Yimei coal group from the perspective of hazards, identifies the hazards based on the above four aspects, and then identifies preventative measures and controls for the identified hazards. CONCLUSIONS: Constructing a safety culture in coal mine enterprises from the perspective of hazards is operable and practical, and thus this study provides essential theoretical and practical value for improving coal mine safety.

Trends and Correlation Between Antimicrobial Resistance and Antibiotics Consumption in a Specialist Children's Hospital from 2016 to 2021.

Purpose: To explore the trends and correlation between antibiotics consumption and antimicrobial resistance in children in a specialist hospital from 2016-2021 in China. Patients and Methods: This retrospective study investigated data on the consumption of antibiotics and antimicrobial resistance in children. Antibiotics consumption was expressed as defined daily doses (DDDs)/1000 patient-days based on the Guidelines for Anatomical Therapeutic Chemical. The trends in antibiotics consumption and antimicrobial resistance rates were analyzed by linear regression, while Spearman correlation analysis was employed to evaluate their correlations. Results: An increasing trend in the annual consumption of carbapenems and monobactams was detected (all P<0.05). A significant upward trend was detected in the annual resistance rates of Enterococcus faecium to ciprofloxacin, Streptococcus pneumonia to ceftriaxone, Acinetobacter baumannii to carbapenems, Enterobacter cloacae to carbapenems, Pseudomonas aeruginosa to ceftazidime, and Escherichia coli to cefepime, while the annual resistance rates of Escherichia coli to carbapenems had a significant downward trend (all P<0.05). The consumption of cephalosporin/ß-lactamase inhibitor (C/BLI) combinations and carbapenems had significant positive correlations with the resistance rates of Acinetobacter baumannii to carbapenems (r=0.763, P<0.001; r=0.806, P<0.001), Enterobacter cloacae to carbapenems (r=0.675, P<0.001; r=0.417, P=0.043), and Pseudomonas aeruginosa to ceftazidime (r=0.625, P=0.001; r=0.753, P<0.001), respectively. Also, increasing consumption of monobactams was related to the upward resistance rates of Acinetobacter baumannii to carbapenems (r=0.557, P=0.005) and Enterobacter cloacae to carbapenems (r=0.507, P= 0.011). Conclusion: This study demonstrated significant positive associations between antibiotics consumption and specific antimicrobial resistance rates. The current findings pointed out some directions to pursue in controlling the prevalence of certain resistant bacterial strains in children.