ABSTRACT
OBJECTIVE: To investigate genetic polymorphisms of 10 short tandem repeats loci (D6S1043, D7S3048, D9S925, D10S2325, D11S2368, D14S608, D15S659, D17S1290, D20S470 and GATA198B05) in Tibetans from Lhasa, China. METHODS: Following extraction, DNA from 208 unrelated Tibetan individuals was amplified with a self-designed multiplex PCR system. The amplified fragments were separated by electrophoresis on an ABI 3130 Genetic Analyzer and analyzed with GeneMapper®3.2 software. RESULTS: The distributions of genotype for the 10 STR loci in the population were in accordance with Hardy-Weinberg equilibrium. The polymorphism information component for the 10 loci was 0.750-0.860, the degree of heterozygosity was 0.726-0.865, the discrimination power was 0.919-0.968, and the probability of exclusion was 0.470-0.725. The combined probability of exclusion and combined discrimination power was 0.9998 and 0.999 999 999 997, respectively. CONCLUSION: Above STR loci have high probability of exclusion and discrimination power, which can be used as candidate markers for population genetic research and forensic practice for Tibetans from Lhasa, China.
Subject(s)
Asian People/genetics , Microsatellite Repeats , Polymorphism, Genetic , China , Genotype , Heterozygote , HumansABSTRACT
Allele frequencies of mitochondrial D-loop (CA)(n) repeat was carried out in six Chinese ethnic groups: Han ethnic group in Beijing, Uygur ethnic group in Kashi of Xinjiang province, Li ethnic group in Hainan province, Yao ethnic group in Junan of Guangxi province, Tibet ethnic group in Lasa of Xizang province and Yi ethnic group in Honghe of Yunnan province. A total of 542 individuals were typed. Comparative analyses between our population data and other populations gathered from the literature were performed.
Subject(s)
Asian People/genetics , DNA, Mitochondrial/genetics , Gene Frequency/genetics , Genetics, Population/methods , Sequence Analysis, DNA/methods , Asian People/classification , Blood Specimen Collection , China , Forensic Genetics/methods , Humans , Polymorphism, GeneticABSTRACT
We have co-amplified (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a,b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y GATA H4) in a single PCR using the AmpFLSTR Yfiler PCR Amplification system. Allelic frequency distribution and haplotype diversity of 17 Y-chromosomal STR from a sample of 131 unrelated individuals of Chinese Han population living in Shandong province of China were obtained. A total of 129 haplotypes were observed in the 131 individuals studied, of which 127 were unique and two were found in two individuals. The gene diversity values ranged from 0.3560 (DYS391) to 0.9675 (DYS385a,b), The overall haplotype diversity for the 17 Y-STR loci was 0.9998, and the discrimination capacity was 0.9695. These results are compared with those observed in worldwide populations at both the locus and the haplotype level.
