Successful percutaneous cannulation of foramen ovale obstructed by completely ossified pterygoalar ligament using the Hartel approach: Two case reports.

RATIONALE: Ossification of the pterygoalar ligament, which lies inferolateral to the exocranial opening of the foramen ovale, is traditionally considered to be a bony bar that could obstruct percutaneous needle access to the foramen ovale using the Hartel approach. We herein present two case reports of successfully penetrating the foramen ovale by a needle across the pterygoalar bar. Lack of knowledge of this type of presentation might lead to a change in the surgical approach. PATIENT CONCERNS: A 27-year-old woman had an 11-year history of facial pain because of a space-occupying lesion in the left cerebellopontine angle. Neither open surgery nor drug therapy resolved her facial pain. Another 67-year-old woman developed episodic facial pain because of herpes zoster infection 20 days earlier, and she could not achieve pain relief from drug therapy. DIAGNOSES: Both patients were diagnosed with secondary trigeminal neuralgia. INTERVENTIONS: The patients underwent radiofrequency thermocoagulation of the semilunar ganglion via the foramen ovale. OUTCOMES: The three-dimensional computed tomography scan showed that the ipsilateral foramen ovale was obstructed by the pterygoalar bar. However, percutaneous needle cannulation of the foramen ovale was successful using the anterior approach. The facial pain was immediately and completely resolved without complications except for facial numbness. LESSONS: During percutaneous radiofrequency thermocoagulation for the treatment of trigeminal neuralgia, the Hartel approach can still be used when the foramen ovale is blocked by a pterygoalar bar. To our knowledge, this is the first report of such a treatment. Moreover, we herein provide specific technical recommendations to assist surgeons who may encounter such cases in the future.

Integrated supramolecular nanovalves for photothermal augmented chemodynamic therapy through strengthened amplification of oxidative stress.

The amplified oxidative stress strategy has been emerged as one promising method to enhance the chemodynamic therapy (CDT) efficacy due to the H2O2 up-regulation and glutathione (GSH) down-regulation behavior in tumor cells. However, how to further achieve the satisfied CDT efficacy is still a big challenge. In this paper, the supramolecular nanovalves (SNs) with oxidative amplification agents cinnamaldehyde-(phenylboronic acid pinacol ester) conjugates (CA-BE) encapsulated inside were developed to accelerate and amplify the generation of ·OH and consumption of GSH while augmenting the CDT efficacy. SNs were obtained through ferrocene/Au modified mesoporous silica nanoparticles (MSN@Au-Fc) and active targeting ß-cyclodextrin modified hyaluromic acid (HA-CD). After CD44 receptor-mediated cellular internalization, the CA-BE were released to elevate H2O2 amount and consume GSH for the desired generation of higher cytotoxic hydroxyl radicals (·OH). Moreover, the NIR-activated MSN@Au-Fc can increase the temperature for the accelerated and amplified oxidative stress. As such, the therapeutic efficacy of our synthesized CA-BE and the accompanied hyperthermia were augmented toward synergistically inhibiting tumor growth.

Effect of rhamnolipids on the biodegradation of m-dichlorobenzene in biotrickling filters: Performance and mechanism.

In this study, the effect of rhamnolipids (RL) on m-dichlorobenzene (m-DCB) removal and biofilm was investigated in two biotrickling filters (BTF) (BTF1: blank control; BTF2: RL addition). The critical micelle concentration (CMC) value of RL was 75.6 mg L-1, and the RL could significantly improve the solubilization of m-DCB. The results showed that the optimal concentration of RL was 180 mg L-1. The removal efficiency (RE) of m-DCB dropped by 42.4% for BTF1 no fed with RL and only 28.2% for BTF2 fed with RL when the inlet concentration increased from 200 to 1400 mg m-3 at an empty bed time (EBRT) of 60 s. RL increased the secretion of extracellular polymers (EPS) and the ratio of Protein/Polysaccharide, which improved the mass transfer of m-DCB to the biofilm. RL also had a facilitating effect on catechol-1,2-dioxygenase (C12O) enzyme activity. Furthermore, RL increased Zeta potential and facilitated microorganisms to form biofilm. The dominant microorganisms of microbial community were increased and the application of RL promoted the enrichment of them.

Supramolecular Self-Assemblies with Self-Supplying H2O2 and Self-Consuming GSH Property for Amplified Chemodynamic Therapy.

Fe-based chemodynamic therapy (CDT) has become one potential method for cancer therapy due to its lower side effect and tumor-specific property. During the process of CDT, the lack of active targeting and biodegradable ability, insufficient endogenous H2O2, and overexpressed GSH in the tumor were responsible for the unsatisfactory therapeutic performance. Hence, we report host-guest interaction-based supramolecular polymers (HGSPs) that were constructed with the biomacromolecule ß-cyclodextrin-grafted hyaluronic acid (HA-CD) as the active targeting host unit and hydrophobic ROS-responsive ferrocene-(phenylboronic acid pinacol ester) (Fc-BE) as the guest unit. HGSPs can further self-assemble into self-assemblies (HGSAs) and encapsulate PA as the prooxidant. After CD44-receptor-mediated cellular internalization, HGSAs could disassemble and release PA to elevate the H2O2 level for the production of higher cytotoxic hydroxyl radicals (•OH) through the Fc-induced Fenton reaction. Moreover, quinone methide (QM) was generated to downregulate antioxidant GSH. The enhancement of H2O2 and consumption of GSH were favorable for CDT due to the amplified oxidative stress. In vivo experimental results indicated that HGSAs@PA might be used as an active targeting amplified CDT agent.

Supramolecular self-assemblies based on water-soluble pillar[6]arene and drug-drug conjugates for the combination of chemotherapy.

Although some co-drug delivery systems have been reported to treat cancer, how to optimal design these nano-systems with enhanced therapeutic efficacy is still a major challenge. As for the nitrogen mustard drugs chlorambucil (Cb), the overexpressed glutathione (GSH) in cancer tissue is responsible for their detoxification and reduced bioavailability. In this paper, chlorambucil-oxoplatin (Cb-Pt) was prepared to fabricate water-soluble pillar[6]arene (WP[6]) based supramolecular drug-drug self-assemblies (SDSAs). Remarkably, after the transcytosis by cancer cells, SDSAs was reduced by GSH to re lease Cb and higher toxic cisplatin, accompanying with the declining GSH level and ascending ROS level. Moreover, in vitro and in vivo experiments demonstrated that SDSAs with oxidative stress amplification strategy exhibited excellent therapeutic effect. This strategy might be useful for the synergistic co-drug based chemotherapy field.

Host-guest interaction-based supramolecular prodrug self-assemblies for GSH-consumption augmented chemotherapy.

The over-expressed cellular glutathione (GSH) severely restricts the chemotherapeutic efficacy due to the GSH-induced detoxification of chemical drugs. Herein, how to construct effective drug delivery systems with GSH-consumption property is still a general concern and a major challenge. In this study, the host-guest interactions between water-soluble pillar[6]arene (WP[6]) and chlorambucil-arylboronic acid (Cb-BA) were utilized to construct supramolecular prodrug self-assemblies (SPSAs) with specific stimuli-responsive property. Notably, the BA moiety could not only consume GSH but also rapidly bind curcumin (Cur), which could inhibit the thioredoxin reductase (TrxR) to further reduce the GSH biosynthesis pathway. Benefiting from the functionality of BA-Cur conjugates, the GSH levels could be significantly downregulated, paving a novel way to enhance chemotherapeutic efficacy. In vitro and in vivo investigations demonstrated that this two-pronged GSH-depletion strategy could amplify the cellular oxidative stress and achieve excellent anti-tumor efficacy.

A study on the correlation of MyD88 expression with gastric cancer.

OBJECTIVE: The myeloid differentiation factor-88 (MyD88) plays a key role in mediating the innate immune signal transduction of toll-like receptor (TLR) and interleukin-1 (IL-1) family members, and it also participates in the regulation of tumorigenesis in various cancer models Our study sought to determine whether there is any correlation with MyD88 and the development of gastric cancer and, if such a correlation exists, to find out whether it can be used to improve the prognosis of gastric cancer patients. PATIENTS AND METHODS: The expression of MyD88 in 108 cases of gastric cancer specimens, 15 cases of adenoma, and 15 cases of normal mucosa was detected by immunohistochemistry, and the correlations of the MyD88 expression with clinicopathologic changes (including disease-free survival [DFS] and overall survival [OS] were analyzed. The level of MyD88 was detected in well-differentiated MGC-803 and poorly-differentiated BGC-823 cell lines by qPCR and western blot. The expression of MyD88 was then measured by western blot after the treatment of an MyD88 overexpression vector or MyD88 inhibitor. Cell proliferation was determined by overexpression or suppression of MyD88. RESULTS: In clinical cases, MyD88 was highly expressed in 23% of patients with gastric cancer as compared to those in normal mucosa and adenoma. There was a significant correlation of MyD88 overexpression with gastric metastasis (P<0.01). The overexpression of MyD88 significantly promoted the proliferation of MGC-803 and BGC-823 cell lines in gastric cancer. According to the single factor analysis, a high expression of MyD88 was strongly associated with poor DFS and OS (P<0.01), and MyD88 was an independent prognostic factor of OS. CONCLUSION: This study demonstrates that a high expression of MyD88 is associated with the gastric cancer patients with liver metastasis, and facilitates the proliferation of gastric cancer cells. MyD88 is an independent predictive factor for the poor prognosis of gastric cancer patients, which provides a potential tool for future clinical diagnosis.