1.
Prikl Biokhim Mikrobiol
; 32(5): 519-23, 1996.
Article
in Russian
| MEDLINE
| ID: mdl-9011859
ABSTRACT
A scheme for purification of hyaluronidase from Staphylococcus aureus 0-15 that allows to produce a 550-fold-purified preparation with a high specific activity was developed. Hyaluronidase was found to consist of two molecular forms with different molecular weights. Staphylococcus hyaluronidase referred to as a complex preparation and its molecular forms have action optimum at pH 5.0-7.2 and at a temperature between 30 and 37 degrees C. The enzymes were highly specific to their substrate: they catalyzed the depolimerization of hyaluronic acid but did not split the other glycosaminoglycans.
Subject(s)
Hyaluronoglucosaminidase/isolation & purification , Staphylococcus/enzymology , Bacterial Proteins/chemistry , Bacterial Proteins/isolation & purification , Hyaluronoglucosaminidase/chemistry
2.
Grudn Khir
; (2): 85-8, 1977.
Article
in Russian
| MEDLINE
| ID: mdl-323116