Molecular characterization and zoonotic potential of Entamoeba spp., Enterocytozoon bieneusi and Blastocystis from captive wild animals in northwest China.

BACKGROUND: Parasites Entamoeba spp., Enterocytozoon bieneusi and Blastocystis are prevalent pathogens causing gastrointestinal illnesses in animals and humans. Consequently, researches on their occurrence, distribution and hosts are crucial for the well-being of both animals and humans. Due to the confined spaces and frequent interaction between animals and humans, animal sanctuaries have emerged as potential reservoirs for these parasites. In this study, the wildlife sanctuary near the Huang Gorge of the Qinling Mountains in northwest China is chosen as an ideal site for parasite distribution research, considering its expansive stocking area and high biodiversity. RESULTS: We collected 191 fecal specimens from 37 distinct wildlife species and extracted genomic DNA. We identified these three parasites by amplifying specific gene regions and analyzed their characteristics and evolutionary relationships. All the parasites exhibited a high overall infection rate, reaching 90.05%. Among them, seven Entamoeba species were identified, accounting for a prevalence of 54.97%, with the highest infection observed in Entamoeba bovis. In total, 11 Enterocytozoon bieneusi genotypes were discovered, representing a prevalence of 35.08%, including three genotypes of human-pathogenic Group 1 and two novel genotypes (SXWZ and SXLG). Additionally, 13 Blastocystis subtypes were detected, showing a prevalence of 74.87% and encompassing eight zoonotic subtypes. All of the above suggests significant possibilities of parasite transmission between animals and humans. CONCLUSIONS: This study investigated the occurrence and prevalence of three intestinal parasites, enhancing our understanding of their genetic diversity and host ranges in northwest China. Furthermore, the distribution of these parasites implies significant potential of zoonotic transmission, underscoring the imperative for ongoing surveillance and implementation of control measures. These efforts are essential to mitigate the risk of zoonotic disease outbreaks originating from wildlife sanctuary.

Dazzling Evaluation of the Impact of a High-Repetition-Rate CO2 Pulsed Laser on Infrared Imaging Systems.

This article utilizes the Canny edge extraction algorithm based on contour curvature and the cross-correlation template matching algorithm to extensively study the impact of a high-repetition-rate CO2 pulsed laser on the target extraction and tracking performance of an infrared imaging detector. It establishes a quantified dazzling pattern for lasers on infrared imaging systems. By conducting laser dazzling and damage experiments, a detailed analysis of the normalized correlation between the target and the dazzling images is performed to quantitatively describe the laser dazzling effects. Simultaneously, an evaluation system, including target distance and laser power evaluation factors, is established to determine the dazzling level and whether the target is recognizable. The research results reveal that the laser power and target position are crucial factors affecting the detection performance of infrared imaging detector systems under laser dazzling. Different laser powers are required to successfully interfere with the recognition algorithm of the infrared imaging detector at different distances. And laser dazzling produces a considerable quantity of false edge information, which seriously affects the performance of the pattern recognition algorithm. In laser damage experiments, the detector experienced functional damage, with a quarter of the image displaying as completely black. The energy density threshold required for the functional damage of the detector is approximately 3 J/cm2. The dazzling assessment conclusions also apply to the evaluation of the damage results. Finally, the proposed evaluation formula aligns with the experimental results, objectively reflecting the actual impact of laser dazzling on the target extraction and the tracking performance of infrared imaging systems. This study provides an in-depth and accurate analysis for understanding the influence of lasers on the performance of infrared imaging detectors.

Damage Mechanism of HgCdTe Focal Plane Array Detector Irradiated Using Mid-Infrared Pulse Laser.

To investigate the damage threshold and mechanism of a mid-infrared HgCdTe focal plane array (FPA) detector, relevant experimental and theoretical studies were conducted. The line damage threshold of a HgCdTe FPA detector may be within the range of 0.59 Jcm-2 to 0.71 Jcm-2. The full frame damage threshold of the detector may be in the range of 0.86 Jcm-2 to 1.17 Jcm-2. Experimental results showed that when the energy density reaches 1.17 Jcm-2, the detector exhibits irreversible full frame damage and is completely unable to image. Based on the finite element method, a three-dimensional model of HgCdTe FPAs detector was established to study the heat transfer mechanism, internal stress, and damage sequence. When HgCdTe melts, we think that the detector is damaged. Under these conditions, the theoretical damage threshold calculated using the detector model is 0.55 Jcm-2. The difference between theoretical and experimental values was analyzed. The relationship between damage threshold and pulse width was also studied. It was found that when the pulse width is less than 1000 ns, the damage threshold characterized by peak power density is inversely proportional to pulse width. This relationship can help us predict the experimental damage threshold of an FPA detector. This model is reasonable and convenient for studying the damage of FPA detectors with a mid-infrared pulse laser. The research content in this article has important reference significance for the damage and protection of HgCdTe FPA detectors.

Excited-state absorption for zinc phthalocyanine from linear-response time-dependent density functional theory.

The mechanism for zinc phthalocyanine (ZnPc) showing optical-limiting character is related to the first singlet excited-state absorption (ESA). Two distinct band peaks in this ESA spectrum (1.97 eV and 2.56 eV) were observed in experiments. However, the origin of the absorption is not well understood. In the present work, we perform accurate quantum mechanical calculations and analysis of the absorption of ZnPc in the first singlet excited state. It is found that the transitions of S1 → S3 and S1 → S24 are the origin of the first and second band peaks, respectively. Charge transfer character is observed between the edges and central parts of ZnPc for those two transitions, but occurs in opposite directions. It is gratifying to note that the absorption can be modified smoothly through the substitution of nitrogen atoms in ZnPc with methyne or benzene rings. The aggregation phenomenon is also investigated with ZnPc dimers. The present calculations show that the absorptions of two ZnPc molecules with cofacially stacked and slightly shifted cofacially stacked configurations both result in an obvious blueshift compared with the zinc phthalocyanine monomer. The present observations may be utilized in tuning the optical-limiting character of ZnPc.

Optical Limiting Properties of Graphene/Polymer Composites.

Graphene oxide (GO) was doped into four polymers films: Poly(methyl methacrylate) (PMMA), polystyrene (PS), polycarbonate (PC), and polyacrylonitrile (PAN). Following that, their optical limiting properties were investigated at 532 nm. In order to make GO hydrophobic, the lipophilic alkyl chains were connected to GO. The results showed that GO/PAN composite possesses better non-linear response than the other three composites at the same transmission (T ~ 59%). The reason were attributed to the thermal effect coming from high input fluence of laser, which improved the cross link density of PAN and further enhanced the interaction between the GO-ODA and PAN. Meanwhile, GO/PC and GO/PS had similar optical limiting property and GO/PMMA film gave the weakest optical limiting effect in our experiment.

Cryptosporidium spp., Giardia intestinalis, and Enterocytozoon bieneusi in Captive Non-Human Primates in Qinling Mountains.

Non-human primates (NHPs) are confirmed as reservoirs of Cryptosporidium spp., Giardia intestinalis, and Enterocytozoon bieneusi. In this study, 197 fresh fecal samples from 8 NHP species in Qinling Mountains, northwestern China, were collected and examined using multilocus sequence typing (MLST) method. The results showed that 35 (17.8%) samples were positive for tested parasites, including Cryptosporidium spp. (3.0%), G. intestinalis (2.0%), and E. bieneusi (12.7%). Cryptosporidium spp. were detected in 6 fecal samples of Macaca mulatta, and were identified as C. parvum (n=1) and C. andersoni (n=5). Subtyping analysis showed Cryptosporidium spp. belonged to the C. andersoni MLST subtype (A4, A4, A4, and A1) and C. parvum 60 kDa glycoprotein (gp60) subtype IId A15G2R1. G. intestinalis assemblage E was detected in 3 M. mulatta and 1 Saimiri sciureus. Intra-variations were observed at the triose phosphate isomerase (tpi), beta giardin (bg), and glutamate dehydrogenase (gdh) loci, with 3, 1, and 2 new subtypes found in respective locus. E. bieneusi was found in Cercopithecus neglectus (25.0%), Papio hamadrayas (16.7%), M. mulatta (16.3%), S. sciureus (10%), and Rhinopithecus roxellana (9.5%), with 5 ribosomal internal transcribed spacer (ITS) genotypes: 2 known genotypes (D and BEB6) and 3 novel genotypes (MH, XH, and BSH). These findings indicated the presence of zoonotic potential of Cryptosporidium spp. and E. bieneusi in NHPs in Qinling Mountains. This is the first report of C. andersoni in NHPs. The present study provided basic information for control of cryptosporidiosis, giardiasis, and microsporidiosis in human and animals in this area.

Mesoporous Transition Metal Oxides for Supercapacitors.

Recently, transition metal oxides, such as ruthenium oxide (RuO2), manganese dioxide (MnO2), nickel oxides (NiO) and cobalt oxide (Co3O4), have been widely investigated as electrode materials for pseudo-capacitors. In particular, these metal oxides with mesoporous structures have become very hot nanomaterials in the field of supercapacitors owing to their large specific surface areas and suitable pore size distributions. The high specific capacities of these mesoporous metal oxides are resulted from the effective contacts between electrode materials and electrolytes as well as fast transportation of ions and electrons in the bulk of electrode and at the interface of electrode and electrolyte. During the past decade, many achievements on mesoporous transition metal oxides have been made. In this mini-review, we select several typical nanomaterials, such as RuO2, MnO2, NiO, Co3O4 and nickel cobaltite (NiCo2O4), and briefly summarize the recent research progress of these mesoporous transition metal oxides-based electrodes in the field of supercapacitors.

Linear equations method for modal decomposition using intensity information.

The linear equations method is proposed to calculate the complete modal content of the partially coherent laser beam using only the intensity information. This method could give not only the incoherent expansion coefficients of the modal decomposition but also the cross-correlation expansion coefficients using the intensity profiles in several planes of finite distance along the propagation direction. A simulation is also presented to verify the validity of this theory. In our algorithm, the minimum and maximum mode orders should be known a priori, so we provide an estimation method for the two parameters.

Nonadiabatic simulation study of photoisomerization of azobenzene: detailed mechanism and load-resisting capacity.

Nonadiabatic dynamical simulations were carried out to study cis-to-trans isomerization of azobenzene under laser irradiation and/or external mechanical loads. We used a semiclassical electron-radiation-ion dynamics method that is able to describe the coevolution of the structural dynamics and the underlying electronic dynamics in a real-time manner. It is found that azobenzene photoisomerization occurs predominantly by an out-of-plane rotation mechanism even under a nontrivial resisting force of several tens of piconewtons. We have repeated the simulations systematically for a broad range of parameters for laser pulses, but could not find any photoisomerization event by a previously suggested in-plane inversion mechanism. The simulations found that the photoisomerization process can be held back by an external resisting force of 90-200 pN depending on the frequency and intensity of the lasers. This study also found that a pure mechanical isomerization is possible from the cis-to-trans state if the azobenzene molecule is stretched by an external force of approximately 1250-1650 pN. Remarkably, the mechanical isomerization first proceeds through a mechanically activated inversion, and then is diverted to an ultrafast downhill rotation that accomplishes the isomerization. Implications of these findings to azobenzene-based nanomechanical devices are discussed.