Transgenerational plasticity in morphological characteristics and biomass of the invasive plant Xanthium strumarium.

Transgenerational plasticity (TGP) allows a plant to acclimate to external variable environments and is a potential mechanism that explains the range expansion and invasion success of some exotic plants. Most studies explored the traits of TGP associated with the success of exotic plant invasions by comparison studies among exotic, native, invasive, and noninvasive species. However, studies on the TGP of invasive plants in different resource environments are scarce, and the biological mechanisms involved are not well understood. This study aimed to determine the role of TGP in the invasiveness of Xanthium strumarium in northeast China. We measured the plant morphology of aboveground parts and the growth of three generations of the invader under different environmental conditions. The results showed that the intergenerational plasticity of X. strumarium was stronger under stress conditions. We found that the X. strumarium parent generation (F0) grown under water and/or nutrient deficiency conditions transferred the environmental information to their offspring (F1 and F2). The F1 generation grown under high-resource conditions has greater height with larger crown sizes, thicker basal diameters, and higher biomass. Both water and nutrients can affect the intergenerational transmission of plant plasticity, nutrients play a more important role compared with water. The high morphological intergenerational plasticity of X. strumarium under a pressure environment can help it quickly adapt to the new environment and accelerate the rapid expansion of the population in the short term. The root:shoot ratio and reproductive and nutrient distribution of the X. strumarium F0 and F1 generations showed high stability when the growth environment of the F0 generation differed from that of the F1 generation. The stable resource allocation strategy can ensure that the obtained resources are evenly distributed to each organ to maintain the long-term existence of the community. Therefore, the study of intergenerational transmission plasticity is of great significance for understanding the invasion process, mechanism, and prevention of invasive plants.

Nitrogen deposition further increases Ambrosia trifida root exudate invasiveness under global warming.

Invasive plants can change the soil ecological environment in the invasion area to adapt to their growth and reproduction through root exudates. Root exudates are the most direct manifestation of plant responses to external environmental changes, but there is a lack of studies on root exudates of invasive plants in the context of inevitable global warming and nitrogen deposition. In this research, we used widely targeted metabolomics to investigate Ambrosia trifida root exudates during seedling and maturity under warming and nitrogen deposition to reveal the possible mechanisms of A. trifida adaptation to climate change. The results showed that the organic acids increased under warming condition but decreased after nitrogen addition in the seedling stage. Phenolic acids increased greatly after nitrogen addition in the mature stage. Most phenolic acids were annotated in the phenylpropane metabolic pathway and tyrosine metabolism. Therefore, nitrogen deposition may increase the adaptability of A. trifida through root exudates, making it more invasive under global warming. The results provide new ideas for preventing and controlling the invasion of A. trifida under climate change.

Effects of different water conditions on the cadmium hyperaccumulation efficiency of Rorippa sylvestris (L.) Besser and Rorippa amphibia Besser.

Increasing the translocation and accumulation of cadmium (Cd) in Cd hyperaccumulator is an important technology to improve the phytoremediation efficiency of Cd-contaminated soil. In order to investigate the effects of different water conditions on the growth and Cd accumulation ability of Cd hyperaccumulators Rorippa sylvestris (L.) Besser and Rorippa amphibia Besser in Cd-polluted soil, clone seedlings of them were transplanted into pots filled with 50 mg kg-1 Cd-contaminated soil and cultured with water conditions of soil relative water content (RWC) 35%, 55%, 75%, 95%, and flooding respectively. The results showed the following: with the increase of RWC, the height of R. sylvestris and R. amphibia increased gradually, the dry biomass of shoot and whole plant increased and reached the maximum in 95% and then decreased in flooding; the Cd concentrations in shoots of R. sylvestris and R. amphibia were more than 100 mg kg-1 except for 35% and flooding; Cd bioconcentration factors (BCFs) of R. amphibia reached the maximum of 3.8870 in 75% and R. sylvestris reached the maximum of 3.2330 in 95%; sufficient water resulted in the decrease of photosynthetic rate due to more Cd accumulation. However, under flooding condition, because of the decrease of Cd bioavailability in soil, the accumulation of Cd in shoots declined and the net photosynthetic rate (Pn) enhanced slightly.

Insecticidal Terpenes From the Essential Oils of Artemisia nakaii and Their Inhibitory Effects on Acetylcholinesterase.

Essential oils (EOs) are often the source of insecticidal substances of high efficiency and low toxicity. From gas chromatograph-mass spectrometer, column chromatography, and nuclear magnetic resonance spectra analyses, twenty terpenes were identified from the EOs of Artemisia nakaii. These comprised mostly monoterpenes (49.01%) and sesquiterpenes (50.76%). The terpenes at the highest concentrations in the EOs of A. nakaii were feropodin (200.46 ± 1.42 µg/ml), (+)-camphor (154.93 ± 9.72 µg/ml), ß-selinene (57.73 ± 2.48 µg/ml), and 1,8-cineole (17.99 ± 1.06 µg/ml), calculated using area normalization and external standards. The EOs were tested for biological activity and showed strong fumigant toxicity and significant antifeedant activity against the larvae of Spodoptera litura. Furthermore, the monoterpenes 1,8-cineole and (+)-camphor displayed significant fumigant activity against S. litura, with LC50 values of 7.00 ± 0.85 and 18.16 ± 2.31 µl/L, respectively. Antifeedant activity of the sesquiterpenes feropodin and ß-selinene was obvious, with EC50 values of 12.23 ± 2.60 and 10.46 ± 0.27 µg/cm2, respectively. The EOs and ß-selinene were also found to inhibit acetylcholinesterase, with IC50 values of 37.75 ± 3.59 and 6.88 ± 0.48 µg/ml, respectively. These results suggest that monoterpenes and sesquiterpenes from the EOs of A. nakaii could potentially be applied as a botanical pesticides in the control of S. litura.