ABSTRACT
OBJECTIVE: To differentiate and identify several species of "Muxiang". METHODS: The main microscopic features and HPLC chromatograms of diagnostic components were studied. RESULTS: Distinguishable characters were found in two methods. CONCLUSION: The results provide reliable and simple reference for the authentication of raw materials of" Muxiang" species, especially the toxic species.
Subject(s)
Asteraceae/ultrastructure , Chromatography, High Pressure Liquid/methods , Plants, Medicinal/ultrastructure , Aristolochic Acids/analysis , Asteraceae/chemistry , Asteraceae/classification , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/standards , Lactones/analysis , Plant Roots/chemistry , Plant Roots/ultrastructure , Plants, Medicinal/chemistry , Quality Control , Reproducibility of ResultsABSTRACT
A DNA probe for Trichosanthes trypsin inhibitor was amplified from the cDNA library of Trichosanthes kirilowii by polymerase chain reaction. Using this probe to screen the cDNA library, a full-length cDNA sequence of the inhibitor was obtained. The open reading frame encodes a Pre-Pro-inhibitor with Pre-, Pro, and mature peptides of 24, 14, and 27 residues, respectively. The deduced amino acid sequence of the mature peptide is consistent with that determined by the primary structure analysis of the protein.
