Immune checkpoints: new insights into the pathogenesis of thyroid eye disease.

Thyroid eye disease (TED) is a disfiguring autoimmune disease characterized by changes in the orbital tissues and is caused by abnormal thyroid function or thyroid-related antibodies. It is the ocular manifestation of Graves' disease. The expression of thyroid-stimulating hormone receptor (TSHR) and the insulin-like growth factor-1 receptor (IGF-1 R) on the cell membrane of orbital fibroblasts (OFs) is responsible for TED pathology. Excessive inflammation is caused when these receptors in the orbit are stimulated by autoantibodies. CD34+ fibrocytes, found in the peripheral blood and orbital tissues of patients with TED, express immune checkpoints (ICs) like MHC II, B7, and PD-L1, indicating their potential role in presenting antigens and regulating the immune response in TED pathogenesis. Immune checkpoint inhibitors (ICIs) have significantly transformed cancer treatment. However, it can also lead to the occurrence of TED in some instances, suggesting the abnormality of ICs in TED. This review will examine the overall pathogenic mechanism linked to the immune cells of TED and then discuss the latest research findings on the immunomodulatory role of ICs in the development and pathogenesis of TED. This will offer fresh perspectives on the study of pathogenesis and the identification of potential therapeutic targets.

Fungal community diversity and their contribution to nitrogen cycling in in-situ aerated landfills: Insights from field and laboratory studies.

The application of in-situ aeration technology in landfills has been reported to promote fungal growth, but the community diversity and function of fungi in the aerated landfill system remain unknown. This study firstly investigated an in-situ aerated remediation landfill site to characterize the fungal community diversity in refuse. And to further reveal the fungal involvement in the nitrogen cycling system, laboratory-scale simulated aerated landfill reactors were then constructed. The results in the aerated landfill site showed a significant correlation between fungal community structure and ammonia nitrogen content in the refuse. Dominant fungi in the fungal community included commonly found environmental fungi such as Fusarium, Aspergillus, Gibberella, as well as unique fungi in the aerated system like Chaetomium. In the laboratory-scale aerated landfill simulation experiments, the fungal system was constructed using bacterial inhibitor, and nitrogen balance analysis confirmed the significant role of fungal nitrification in the nitrogen cycling process. When ammonia nitrogen was not readily available, fungi converted organic nitrogen to nitrate, serving as the main nitrification mechanism in the system, with a contribution rate ranging from 62.71 % to 100 % of total nitrification. However, when ammonia nitrogen was present in the system, autotrophic nitrification became the main mechanism, and the contribution of fungal nitrification to total nitrification was only 15.96 %. Additionally, fungi were capable of directly utilizing nitrite for nitrate production with a rate of 4.65 mg L-1 d-1. This research article contributes to the understanding of the importance of fungi in the aerated landfill systems, filling a gap in knowledge.

Deep-dewatering of sewage sludge using double dielectric barrier discharge (DDBD) plasma technology.

Deep dewatering of sewage sludge is essential for optimizing disposal and resource recovery. This study explores the potential of Double Dielectric Barrier Discharge (DDBD) plasma for enhancing waste activated sludge (WAS) dewatering. Key operational parameters (applied voltage, treatment duration, and air feeding rate) were systematically investigated using a two-step approach: Single Factor-at-a-Time (SFAT) and central composite design (CCD) within the response surface methodology (RSM) framework. The aim was to identify influential factors and their optimal settings for maximizing dewatering efficiency while minimizing energy usage. Higher applied voltages (30 kV) and longer treatment durations (40 min) notably improved % moisture reduction (%MR) (92.92 % and 94.35 %, respectively). ANOVA analysis emphasized the equal and substantial impact of applied voltage and treatment duration on %MR and energy efficiency (EE), whereas the air feeding rate exhibited no significant effect. However, it's worth noting that %MR and EE did not display a strictly linear relationship, suggesting complex interactions. Furthermore, two soft sensing models were developed: a quadratic model for %MR and a linear model for energy efficiency (EE). Results showed minimal reductions in TOC content, maintaining values between 13.68 % and 14.28 % compared to untreated sludge 14.37 %. The study also revealed that ROS generated by DDBD plasma played a key role in sludge disintegration, as observed through SEM and FTIR, enhancing dewatering efficiency by the destruction of sludge flocs and the transformation of organic substances. In conclusion, DDBD plasma technology offers a sustainable solution for effective sludge dewatering in WWTPs, preserving organic content.

Effect of temperature on fungal nitrification in simulated in-situ aeration of aged MSW landfill.

Fungal nitrification is one kind of heterotrophic nitrification that involves certain species of fungi promoting the transformation of organic nitrogen and ammonia nitrogen to nitrite/nitrate. In this study, simulated aerated landfill reactors (SALRs) were constructed to investigate fungal nitrification in aged municipal solid refuse, with a focus on understanding the effect of temperature on the performance of fungal nitrification as well as fungal contribution to ammonia nitrogen transformation. Different nitrogen metabolism patterns have been observed in the system with fungi only (SALRF) and complete microbial consortium, i.e., bacteria + fungi (SALRC). At a temperature of 35 °C, autotrophic nitrification dominated the ammonia nitrogen transformation, while fungal nitrification did not significantly contribute to ammonia removal. However, at elevated temperatures (i.e., 45 °C and 55 °C), fungi played a crucial role in ammonia transformation through fungal assimilation and fungal nitrification, with bacterial function suppressed. Furthermore, 45 °C was found to be the optimal temperature for fungal nitrification, exhibiting the highest nitrification rate (13.98 mg L-1 d-1) which accounted for 49.80% of total nitrification rate in the aerated landfill. High throughput sequencing revealed reshaping of fungal community in response to temperature variation. The abundance of Aspergillus fumigatus, with a relative abundance ranging from 67.13% to 92.71% at elevated temperatures, suggested its significant potential for fungal nitrification. These findings have implications for the promotion of nitrogen cycle through strengthening fungal nitrification in aerated landfill sites which often operate at high temperatures.

Radiomics analysis of T1WI and T2WI magnetic resonance images to differentiate between IgG4-related ophthalmic disease and orbital MALT lymphoma.

BACKGROUND: Preoperative differentiation between IgG4-related orbital disease (IgG4-ROD) and orbital mucosa-associated lymphoid tissue (MALT) lymphoma has a significant impact on clinical decision-making. Our research aims to construct and evaluate a magnetic resonance imaging (MRI)-based radiomics model to assist clinicians to better identify IgG4-ROD and orbital MALT lymphoma and make better preoperative medical decisions. METHODS: MR images and clinical data from 20 IgG4-ROD patients and 30 orbital MALT lymphoma patients were classified into a training (21 MALT; 14 IgG4-ROD) or validation set (nine MALT; six IgG4-ROD). Radiomics features were collected from T1-weighted (T1WI) and T2-weighted images (T2WI). Student's t-test, the least absolute shrinkage and selection operator (LASSO) and principal component analysis (PCA) were conducted to screen and select the radiomics features. Support vector machine (SVM) classifiers developed from the selected radiomic features for T1WI, T2WI and combined T1WI and T2WI were trained and tested on the training and validation set via five-fold cross-validation, respectively. Diagnostic performance of the classifiers were evaluated with area under the curve (AUC) readings of the receiver operating characteristic (ROC) curve, and readouts for precision, accuracy, recall and F1 score. RESULTS: Among 12 statistically significant features from T1WI, four were selected for SVM modelling after LASSO analysis. For T2WI, eight of 51 statistically significant features were analyzed by LASSO followed by PCA, with five features finally used for SVM. Combined analysis of T1WI and T2WI features selected two and four, respectively, for SVM. The AUC values for T1WI and T2WI classifiers separately were 0.722 ± 0.037 and 0.744 ± 0.027, respectively, while combined analysis of T1WI and T2WI classifiers further enhanced the classification performances with AUC values ranging from 0.727 to 0.821. CONCLUSION: The radiomics model based on features from both T1WI and T2WI images is effective and promising for the differential diagnosis of IgG4-ROD and MALT lymphoma. More detailed radiomics features and advanced techniques should be considered to further explore the differences between these diseases.

Enhanced ethanol-driven carboxylate chain elongation by Pt@C in simulated sequencing batch reactors: Process and mechanism.

Carboxylate chain elongation can create value-added bioproducts from the organic waste. The effects of Pt@C on chain elongation and associated mechanisms were investigated in simulated sequencing batch reactors. 5.0 g/L of Pt@C greatly increased the synthesis of caproate, with an average yield of 21.5 g COD/L, which was 207.4% higher than the trial without Pt@C. Integrated metagenomic and metaproteomic analyses were used to reveal the mechanism of Pt@C-enhanced chain elongation. Pt@C enriched chain elongators by increasing the relative abundance of dominant species by 115.5%. The expression of functional genes related to chain elongation was promoted in the Pt@C trial. This study also demonstrates that Pt@C may promote overall chain elongation metabolism by enhancing CO2 uptake of Clostridium kluyveri. The study provides insights into the fundamental mechanisms of how chain elongation can perform CO2 metabolism and how it can be enhanced by Pt@C to upgrade bioproducts from organic waste streams.

Valorization of hexoses into 5-hydroxymethylfurfural and levulinic acid in acidic seawater under microwave hydrothermal conditions.

Typical value-added platform chemicals 5-hydroxymethylfurfural (HMF) and levulinic acid (LA) can be obtained from hexoses under microwave hydrothermal (MHT) conditions. This study explored the detailed transformation process regarding the MHT products in acidic seawater obtained using glucose and fructose as raw materials. The facile conversion of fructose compared with glucose was mainly ascribed to their different activation energies (56.721 and 88.594 kJ mol-1, respectively). The HMF and LA product yields were strongly affected by the MHT temperature and holding time in two types of hexose solution. Undesirable humins were found to inevitably form under each set of reaction conditions. The carbon balance results for reactants and products showed that up to 60% of fructose carbon was converted into value-added chemicals, while 47% of glucose carbon underwent the same conversion in acidic seawater under the optimal MHT conditions. This study provides further knowledge regarding the role of microwave heating combined with acidic seawater in green chemistry and is a useful reference for the biorefinery industry.

Strategy of electron acceptors for ethanol-driven chain elongation from kitchen waste.

A two-phase kitchen waste (KW) fermentation was proposed in the current study to enhance medium-chain fatty acids (MCFAs) production from kitchen waste. In particular, effect of acetate to butyrate ratio (ABR) on MCFAs production was investigated which can be regulated by different pH and organic loading during the acidification phase. Medium ABR (1.00) was obtained when pH is 5.5 and organic loading is 20 g VS/L in FW acidification fermentation. Subsequent chain elongation fermentation demonstrated that the highest yield of caproate 9.67 g/L with selectivity of 79 %, and highest ethanol conversion efficiency of 1.11 was achieved in medium ABR system. Microbial community study showed that medium ABR significantly enrich the functional bacteria especially Clostridium kluyveri. The study provides a new method for chain elongation enhancement without addition of other additives in kitchen waste fermentation system and gives a guide for the regulation of the short-chain fatty acids distribution in its acidification phase.

Humic Substances Derived From Biomass Waste During Aerobic Composting and Hydrothermal Treatment: A Review.

Humic substances (HSs) occupy 80% of organic matter in soil and have been widely applied for soil remediation agents, potential battery materials, and adsorbents. Since the HS extraction rate is very low by microbial degradation in nature, artificial humification processes such as aerobic composting (AC) and hydrothermal treatment (HT) have attracted a great deal of attention as the most important strategies in HS production. This article aims to provide a state-of-the-art review on the development of conversion of biomass waste into HSs based on AC and HT for the first time in terms of mechanisms, characteristics of HSs' molecular structure, and influencing factors. In addition, some differences based on the aforementioned information between AC and HT are reviewed and discussed in the conversion of biomass waste into HSs in a pioneering way. For biomass waste conversion, a feasible strategy on effective humification processes by combining AC with HT is proposed.

Advanced remediation of pyrene contaminated soil by double dielectric barrier discharge (DDBD) plasma and subsequent composting process.

Due to increasing industrialization, soils are increasingly contaminated by polycyclic aromatics such as pyrene and need gentle treatment to keep the soil functioning. This study applied a double dielectric barrier discharge (DDBD) plasma reactor and composting reactor to remediate pyrene-contaminated soil. The effect of peak-to-peak applied voltages on the remediation efficiency of pyrene was investigated. The experimental results illustrate that pyrene remediation efficiency increased from 43% to 85% when the peak-to-peak applied voltage was increased from 28.0 to 35.8 kV. When using the combined method of DDBD and composting, 90-99% of pyrene could be removed, while a reduction of 76.5% was achieved using only composting, indicating the superiority of the combined system. Moreover, the authors could demonstrate that DDBD plasma treatment improves humification in the post-composting process as humic acid (HA) concentrations increased to 7.7 mg/g with an applied voltage of 35.8 kV; when composting was used as the sole treatment method, only 3.4 mg/g HA were produced. The microbial activity in the DDBD plasma-treated soil peaked on the 5th day and had a 2nd rise afterwards. The authors demonstrate that the combined technology of DDBD plasma and composting is a promising method for soil remediation with persistent organic pollutants. This treatment approach improves pollutant degradation efficiency and facilitates further humification, potentially restoring the function of contaminated soil. This approach could be considered a cost-effective and green strategy for soil remediation with persistent organic pollutants.

The formation of 5-hydroxymethylfurfural and hydrochar during the valorization of biomass using a microwave hydrothermal method.

5-Hydroxymethylfurfural (HMF) and levulinic acid (LA) are regarded as value-added platform chemicals that can be derived from biomass waste. However, humins are inevitably produced during valorization processes, reducing the product yields. Previous studies indicated that microwave heating combined with acidic seawater as a reaction medium promotes HMF formation. The present work therefore investigated the relationship between the production of HMF and LA in the liquid phase and that of insoluble humins (that is, hydrochar) under microwave heating in acidic seawater. The selectivities for HMF and LA were found to decrease as the reaction time was increased, as a result of hydrochar formation, and both dehydration and decarboxylation evidently dominated the production of hydrochar in succession. HMF evidently played the most important role in hydrochar formation, and was consumed approximately seven times more rapidly than either fructose or LA. The hydrochar formation mechanism reported herein may be applicable to other similar hydrothermal processes.

Acidic seawater improved 5-hydroxymethylfurfural yield from sugarcane bagasse under microwave hydrothermal liquefaction.

5-Hydroxymethylfurfural (HMF) as value-added platform chemical can be derived from biomass. This study used microwave hydrothermal liquefaction (MHTL) to obtain HMF from sugarcane bagasse in acidic seawater conditions. The key processing parameters including temperature, reaction time, and liquid-to-solid ratio (L/S) were evaluated and optimized. The highest HMF yield of 8.1 wt% was obtained at 149 °C with a reaction time of 4 min and a L/S value of 12:1, respectively. This yield is considerable and even higher than the yield derived from sugarcane molasses under similar microwave conditions in the absence of seawater. Hence, acidic seawater was found to promote the hydrolysis of sugarcane bagasse to give HMF precursor (i.e. fructose and glucose), while simultaneously inhibiting the conversion of HMF to levulinic acid under MHTL conditions, possibly explaining the high HMF yield. This method presents a new and sustainable means of transforming waste biomass to valuable substances using seawater or brine wastewater.