ABSTRACT
Objective: To explore the operation method and clinical effect of treatment for bilateral cleft lip with improved straight line closure. Methods: On the basis of straight line closure, the vermilion and anterior lip pear were repaired with the muscle-vermilion labial flap of lateral lip. In order to close nasal floor, flap-C from lateral wall of anterior lip and flap-G from nasal wing base were designed, meanwhile, nasal mucosa flap-D and flap-H from two sides of nasal base fissure were designed. The function of the orbicularis oris was repaired by cutting off the anomaly adhesion of the orbicularis oris. Results: The post-operational upper lip was thickened together with good shape and vermilion color, there was no obvious dynamic abnormality, nasal floor was closed well. Conclusion: The improved straight line closure can obtain good shape of lip, reduce the whistling deformities, oronasal fistula and dynamic abnormality.
ABSTRACT
Objective To further explore the mechanism by which bone morphogenetic protein 4(BMP-4)might be involved in hematopoietic differentiation of the yolk sac.We observed the expression of BMP-4,CD34,CD133 and tyrosine kinase receptors(KDR) in the blood island of the yolk sac at embryonic 3 to 8 weeks.Methods Gene expression was analyzed by RT-PCR and the presence of BMP-4,CD34,CD133 and KDR proteins was confirmed by immunohistochemistry in 57 human embryos.Results In the human yolk sac,we found that BMP-4 was expressed at high levels from the 16th day to the 7th week,and decreased quickly after week 7.The results showed that KDR,CD133 and CD34 largely appeared on the 21st and 30th day,then increased at the 6th week,and decreased quickly after week 7.Furthermore,Ihh,SCl,GATA-1,GATA-2 and PU.1 mRNAs showed that PU.1 was not expressed on the 16th day;however,other factors were expressed all the time.Conclusion The distribution of BMP-4,KDR,CD34,CD133 and transcription factors expression highly suggested that BMP-4 was secreted from the yolk sac which might exert its effects on the specification of human hemangioblast and hematopoietic stem cells from the embryonic mesoderm in vivo through transcription factors.
